FastQCFastQC Report
Fri 2 Oct 2020
HTGTLBGXG_n02_S2_R2_D12_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTGTLBGXG_n02_S2_R2_D12_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences42
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACATTTGAGACTCGCCAAATAATAAAACTTGTCGCCTTTCCTGCGGCCCC12.380952380952381No Hit
GCAAAGGTCAACCTCATACCAAGTCGCCCATTTGATGCGGGCCACCAACA12.380952380952381No Hit
GATCCAAATTGGATGACTTTGTGGGCCGATGCTTCCATCCACTGATCGTC12.380952380952381No Hit
GTCAACTTCTCAGGTCCGTTCGTTTCACTGACTTCTTCGGGATCCAATAG12.380952380952381No Hit
CAATAGGGAAGAAAAAACAAAGACTGAAGAAGAGAGGCGATCTAATAAGA12.380952380952381No Hit
ACCCAGGAGATTTCATCGATTAGGAGGAGCTAAGAGAGCAATTGAGCTCA12.380952380952381No Hit
GATTGGAGGAGTAAGGATGGTGGACATCCTTAGACAGAATCCAACTGAGG12.380952380952381No Hit
GCGTGGGAAGGGTTTGGCGGGTGGCGTGTCTTTGGCGGGTGGCGGGGGCT12.380952380952381No Hit
CCCTTGGGTTAAGTGCAACACTTCAATATAAAGCCTGCCTGTTCCGCCGG12.380952380952381No Hit
CTTGACTCGCTGCATCTGCCCTCCCCTTCGCTTCGGGTAGGCCTGCAAAT12.380952380952381No Hit
GTAATCCACAATATCAGGTGCTAGATCCCAATGATATTTGCTGCAATGAC12.380952380952381No Hit
CTACCGATAAGTTCAATCCCTCCAACAAATAATAATATTTAATCAAGTTA12.380952380952381No Hit
ATTGTCCACTTCTCAGTTCATTGCGTTTCACTGACTTCTTCGGGAGACCA12.380952380952381No Hit
ATCTAAGGTAGCAGTCAAGCACTCGCGAGATACTCACTAAGACCAATTTG12.380952380952381No Hit
CTTTAACGCAGGTGAGGTTCTGGTCTGAATGTGTGTGGCTAGCGTAGGGC12.380952380952381No Hit
CTACTCTGTTCTGGTGGGGCAGCAGCAACGGGGAGAAGTGTTATTCTTTG12.380952380952381No Hit
GGGCGGCATTGCTTCAATTAACCACCGTATTTCCTCAAAGTTCTGTCCCA12.380952380952381No Hit
CTGGTTCGGATTTGTGCCTTGCTCCTCGGCTGGGGGTGTATCTCCGCTTG12.380952380952381No Hit
TGCATGTACTCTTATTTTCAGTGTGTGGAGGTTGCCCGTTAGCACTTCTT12.380952380952381No Hit
CTTTCGCTAGTCTCCTTTGCTCAAAGCGTTACCCGTCTTGGCTCCCAGTA12.380952380952381No Hit
GTATCAGATGGAGGACCAAACTTATACAATATACGGCATCTTCACAGTCC12.380952380952381No Hit
CCCTAAGGTATAAAAAACTTATTTCGAAAAGGTCGATAGGTTGAAACATG12.380952380952381No Hit
TCTCGATACTGAATCTTGGACAAAAGAAATACCCCAAGACAATATACTGG12.380952380952381No Hit
GGTTTGGGCCCAGGGGTTGGTTATCTCAATTGATCATGTGGTCTCCATTT12.380952380952381No Hit
GCTGATGGATGCTCTGAAATTAAGTATTGAAGCCCCGAGTCGCGCGGGGG12.380952380952381No Hit
AAATAACTCATTATATGTTAGTAAAATAGAACTAAACACTAAAATGGTGT12.380952380952381No Hit
GGTCTCATTTGAGAAATGATACTGATGTGGTGAACTTTGTAAGTATGGAG12.380952380952381No Hit
TCTCAGGACAGCCGCCTAACCCCAAGATGGGGGTTAATCTGGCTATGCAG12.380952380952381No Hit
ACGACTGACTCTGTGACAGATTTCAAGCGCTCAGTATTTATCTCAATATG12.380952380952381No Hit
ATTAGGATCAGATGGGGGACCAAACTTATAAAATATACGGAATCTTCACA12.380952380952381No Hit
GTTTAATGTCCAATCATAAGTCTGGCGACCTTGAGTTAGTTTATCTACCC12.380952380952381No Hit
AGAGATAACAACCCACTTTCAAAGAAAAAGGAGAGTAAGAGACAACCTGA12.380952380952381No Hit
ATTATTGCCTCAGCAATTGACTGCTCGTCTCTCCCGCTTACTATCAACTG12.380952380952381No Hit
TTCTCACCGAGGTCGAAACGTACGTTCTTTCTATCATCCCGTCAGGCCCA12.380952380952381No Hit
GTATATGTGTTAGAATAATCATTCTTTTACTCTAGAACATGTACCATTTC12.380952380952381No Hit
CATTTTTGGTGCCATTCTCAGGATCTTTCTGAACCAATATGTTTGATTTC12.380952380952381No Hit
GAGAAGCAGCGACTGGGCACTAAGGACCAGGAGTGGAGGCAATACCACTC12.380952380952381No Hit
GTCTAAGGATGTCCACCATCCTTACTCCTCCAATCTGTGTGGTGTGGCAC12.380952380952381No Hit
CTATTGGGCCCTCCTCAGTGCGCGCCCGTAGGAGTAGCAAGGTGTCTAAT12.380952380952381No Hit
ACCATGTACTAAAACCTGAGAGAAATTCAAAACCGTCTACTATATTAACT12.380952380952381No Hit
AGGTAGTTTTTTACTCTAGCTCTATGTTGACAAAATGCCCATCGTAAACA12.380952380952381No Hit
GCCCATGGTCCAGCCAAACGCATTGAATATGATGCCGTTGCAACTACACA12.380952380952381No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)