FastQCFastQC Report
Fri 2 Oct 2020
HTGTLBGXG_n02_S2_R2_D11_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTGTLBGXG_n02_S2_R2_D11_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3066
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATCCGAAT60.19569471624266144No Hit
TCCTTAGACAGAATCCAACTGAGGAACAAGCCGTAGACATATGCAAGGCA50.16307893020221786No Hit
CTTATCTCTTGTTCTACTTCAAGCAGTAGTTGTAAGGCTTGCATAAATGT50.16307893020221786No Hit
TTTTTAAACAATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCG50.16307893020221786No Hit
CACTATGAGAGCAAAATCGTCGGATGATTGGAGCCCATCCCACCAGTATA40.1304631441617743No Hit
GGTCAAATATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATG40.1304631441617743No Hit
CATATAGGTGCCATAGGGGAGACACACAAATTCAGACGAGAAGATCATTT40.1304631441617743No Hit
TAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACTCGCGAGATACTC40.1304631441617743No Hit
GCTTAATGCTGGGCCATATCTCTTGTCTTCTTTGCCCAAAATGAGAAATC40.1304631441617743No Hit
GAACTGAGAGATCTAATGTCGCAGTCCCGCACTCGCGAGATACTCACTAA40.1304631441617743No Hit
ATATAAATAAGACAGGGACATTTGAATTCACAAGCTTTTTTTATCGCTAT40.1304631441617743No Hit
ATGTTATACAACAAAATGGAATTTGAACCATTTCAGTCTCTTGTCCCTAA40.1304631441617743No Hit
GTCTAAGGATGTCCACCATCCTTACTCCTCCAATCTGTGTGCTGTGGCAC40.1304631441617743No Hit
GTATAAGTTTGGTCCTCCATCTGATACTAATAGCCCTACCTTTGATTGGG40.1304631441617743No Hit
ATATAGTGGTATTCCCTCCCCCTCGTGACTCGGGTCTTCAATACTTAATT40.1304631441617743No Hit
AGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACAATGCTTCAATCC40.1304631441617743No Hit
CTTACAAAGTTCACCACATCAGTATCATTTCTCAAATGAGACCTTCCTTT40.1304631441617743No Hit
TTCTTGATCCGTCCAGACTCGAAGTCGACCCTGGCATCAATCCGGGCCCT40.1304631441617743No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACAGTAT100.006750369145.08
ACTTTAG100.006750369145.07
Produced by FastQC (version 0.11.5)