FastQCFastQC Report
Fri 2 Oct 2020
HTGTLBGXG_n02_S2_R2_D10-FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTGTLBGXG_n02_S2_R2_D10-FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1315432
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTATATACAGCTCGCTGAAGAGGTCCATGACCTCAAAAAAGATCAAATG46310.35205164539101985No Hit
GTATATACATACATATATACTTACACAGTTTGAGCATTCACAGGCCACTA45090.34277712568950736No Hit
CATATATACTTACACAGTTTGAGCATTCACAGGCCACTAGAAGCCCATCT38080.28948664773245597No Hit
GTATATATGTATGTATATACAGCTCGCTGAAGAGGTCCATGACCTCAAAA31000.23566402520236698No Hit
GTCCTAAGTTGTAAGCAGTGTTTTGACTGTTGTAGGTGGCGCCTGCTGTG17720.13470859763180462No Hit
CTATAAAACATAATGTAATAGCATCACATTTCTTAAGTTAATGTTGGGGA17400.13227593672648985No Hit
TACTTACACAGTTTGAGCATTCACAGGCCACTAGAAGCCCATCTGTGGCA17300.13151573019357898No Hit
GTTGTAGGTGGCGCCTGCTGTGAGCATCTGTACGAATTTCGAGTTTTTGT15650.1189723224005498No Hit
ATGTAATAGCATCACATTTCTTAAGTTAATGTTGGGGAATATACTGGGGT15230.11577945496232417No Hit
TCCTTAGGGTGATCCCAGGGCTTTTGTCATTCTGTCTTCTCCATTCCCAC15140.11509526908270439No Hit
ACTTAACACAAGGTGCCTAGAGTCCTCAAGCTCATAGAGACAGAGAAATA14380.10931769943258185No Hit
GTCCATGAGCATGTCTGGTTTTACAACTCTTGTCAGAGGCTCAGACACTC14310.10878555485954425No Hit
CATATACACAGAATGTGGCTTAAACATGGGATCATAGGTCTAAGCGGGCA13860.10536462546144537No Hit
GTTTTATAGTATCTGTTGAGAAAAGAAGTGACTGAAGAGAAAATACAAAA13430.10209573736992866No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGTTA256.4627184E-6116.01053145
CGAAAGC6000.080.922583
GGATCTA554.3673936E-879.164361
CATACCG851.8189894E-1276.701635
GGATAGC1600.068.0318761
TAATAGG450.009663948.30284
CATACAT17250.046.1903848
GCGAAAG10850.046.1242182
CGAATTG803.7551632E-545.2718249
GATAGCA2700.042.9799462
AGCGAAA12000.042.3309441
ATATATG17800.041.5265923
TACATAC18850.041.120036
ATACCGA2300.040.944596
ATGTATG14850.039.0251437
GTAAGGT2500.037.6833463
ACCGAAA1803.2305252E-936.217468
CCGAAAT1803.2305252E-936.217469
ACATACA21950.035.9726947
TATATGT19200.035.8497354
Produced by FastQC (version 0.11.5)