FastQCFastQC Report
Fri 2 Oct 2020
HTGTLBGXG_n02_S2_R2_D08_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTGTLBGXG_n02_S2_R2_D08_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1297722
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTATATACAGCTCGCTGAAGAGGTCCATGACCTCAAAAAAGATCAAATG45840.35323435990142726No Hit
GTATATACATACATATATACTTACACAGTTTGAGCATTCACAGGCCACTA44770.3449891425128032No Hit
CATATATACTTACACAGTTTGAGCATTCACAGGCCACTAGAAGCCCATCT37790.2912025842206574No Hit
GTATATATGTATGTATATACAGCTCGCTGAAGAGGTCCATGACCTCAAAA30810.23741602592851166No Hit
GTCCTAAGTTGTAAGCAGTGTTTTGACTGTTGTAGGTGGCGCCTGCTGTG17630.13585344164620775No Hit
CTATAAAACATAATGTAATAGCATCACATTTCTTAAGTTAATGTTGGGGA17310.1333875822402641No Hit
TACTTACACAGTTTGAGCATTCACAGGCCACTAGAAGCCCATCTGTGGCA17190.1324628849630352No Hit
GTTGTAGGTGGCGCCTGCTGTGAGCATCTGTACGAATTTCGAGTTTTTGT15560.11990241361400979No Hit
ATGTAATAGCATCACATTTCTTAAGTTAATGTTGGGGAATATACTGGGGT15070.11612656639865857No Hit
TCCTTAGGGTGATCCCAGGGCTTTTGTCATTCTGTCTTCTCCATTCCCAC15010.11566421776004414No Hit
ACTTAACACAAGGTGCCTAGAGTCCTCAAGCTCATAGAGACAGAGAAATA14270.10996191788379946No Hit
GTCCATGAGCATGTCTGGTTTTACAACTCTTGTCAGAGGCTCAGACACTC14200.10942251113874929No Hit
CATATACACAGAATGTGGCTTAAACATGGGATCATAGGTCTAAGCGGGCA13750.10595489634914104No Hit
GTTTTATAGTATCTGTTGAGAAAAGAAGTGACTGAAGAGAAAATACAAAA13340.10279551398527573No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAAAGC6800.077.780183
GGATAGC1850.050.9500661
GCGAAAG11350.045.9771772
GATAGCA2300.044.1169932
AGCGAAA12200.042.790341
ACATCGA700.001060351541.399998
TACAGGA3200.040.7546844
CATACCG1252.7361966E-740.5688635
GTATTAC5900.040.5541231
CATACAT18050.039.7371148
TACATAC18550.038.663056
GTAAGGT2002.2555469E-1036.226393
GGGCGTC1001.397655E-436.22367
ATACCGA2252.0008883E-1135.4172636
ATATATG17250.034.4413223
GCCTAAG5300.034.200881
CCGAAAT1501.140028E-633.8112959
TTTAGGC1301.6625565E-533.4397433
ACATACA21600.033.2049647
ATGTATG13750.033.1939857
Produced by FastQC (version 0.11.5)