FastQCFastQC Report
Fri 2 Oct 2020
HTGTLBGXG_n02_S2_R2_D06_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTGTLBGXG_n02_S2_R2_D06_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3153
Sequences flagged as poor quality0
Sequence length151
%GC45

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AATATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCA40.12686330478908975No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGGGGA100.0067611145.06
GAATGGG100.0067611145.04
TGGGGAC100.0067611145.07
TACAATT100.0067611145.07
AATGGGG100.0067611145.05
GGGACCC100.0067611145.09
GGAATGG100.0067611145.03
GGGGACC100.0067611145.08
ATACAAT100.0067611145.06
Produced by FastQC (version 0.11.5)