FastQCFastQC Report
Fri 2 Oct 2020
HTGTLBGXG_n02_S2_R2_D06-FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTGTLBGXG_n02_S2_R2_D06-FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1366866
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCTTAGACAGAATCCAACTGAGGAACAAGCCGTAGACATATGCAAGGCA17230.12605478518011276No Hit
GTCTAAGGATGTCCACCATCCTTACTCCTCCAATCTGTGTGCTGTGGCAC16830.12312838273832256No Hit
CCTATATGAACTGCTAGGGAAAAATTTCTCGAATAGATTGCAGCACTTCT16000.1170560976716079No Hit
ATATAAACACTGCCTGTTCCGCCGGCTACTGGGAGAAACCTTGTTTTACG15830.11581237663384705No Hit
TTTTTAAACAATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCG14800.1082768903462373No Hit
CCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATACA14400.10535048790444712No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA2650.060.2099531
AGTAGAA8750.053.0473141
CGAAAGC3900.042.718313
CCTTATA6900.037.8089832
GTAGAAA12500.037.6829532
GCGAAAG4250.037.512442
CAATTCG5450.037.2131549
CAGGTAC5750.035.272893
TTATACT7400.034.253724
TATATTC7900.033.925443
GGTACTG5850.033.4243855
GACCGTC900.003646528332.1899767
ATATTCA8600.030.316254
GACAGTA4650.029.594017
GGACCGT1255.188444E-428.9677986
TCTACAC3850.028.2215843
GTCTAAG10550.027.4978681
CCCTTAT9500.027.4833971
ACTATAT9250.027.4079883
GCAGGTA7550.026.8752612
Produced by FastQC (version 0.11.5)