FastQCFastQC Report
Fri 2 Oct 2020
HTGTLBGXG_n02_S2_R2_D05_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTGTLBGXG_n02_S2_R2_D05_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGGTAGTTTTTTACTCTAGCTCTATGTTGACAAAATGACCATCGTCAACA12.941176470588235No Hit
ATTCTCAATTGTTATGTGGATGTTCATTTCTTTCAATCATTATGAGGTCT12.941176470588235No Hit
GAGGAAATAGAGATAACCACCCACTTTCAAAGAAAAAGGAGAGTAAGAGC12.941176470588235No Hit
CAGTCGAGCAGTTTGATATTCATTCCACTGAGTGTTGTCACCCGTGATTG12.941176470588235No Hit
TTCTTGATCCGTCCAGACTCGAAGTCGACCCTGGCATCAATCAGGGCCCT12.941176470588235No Hit
CACGCTGCCCGTGGAGTTCAATCAGTCACTAAAGGGAACCTCTTAGCTCA12.941176470588235No Hit
ACTATCAACAGTATGAATAGATGTGAATGATAGTCTTCTCAAATTTTATA12.941176470588235No Hit
NNCTTATTGAATTCATTTTGGACCCAGGTTGGTAGAGATCTGGGCTCTGG12.941176470588235No Hit
AAAGTACACATCAGGAAGGCAAGAGAAGAACCCCGCACTCAGAATGAAGT12.941176470588235No Hit
GACCCAATGGAACCTCCAAGATCAAGATGAAATGGGGCATGGAAATGAGG12.941176470588235No Hit
AATTTGCAATATTGGTACATTTATTATGATTTAAATTTCAACCACGAACG12.941176470588235No Hit
AAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAACGAGAAAGCTCTTA12.941176470588235No Hit
GCTGGATTTCTGTTGTCGGACAGCATATGCTGAGGGCTGCCAGTGACCCC12.941176470588235No Hit
TGTTTTACGGACCAATTCTCTTTCTAGCATGTACGCCACCATCAAGGGAG12.941176470588235No Hit
ATTTCTTTCACAATCACTGGGGACAACACTAAGTGGAATGAAAATCAAAA12.941176470588235No Hit
CAACTGGATCAATCTCCTGGTTGCCTTTCTGAGAATAGCTGTTGCTCTTC12.941176470588235No Hit
GCTCAGTTCATTGATGCTTAATGCTGGGCCATGTCCATTATTCTCTTGCT12.941176470588235No Hit
GATACAAACCCTGGCCATGCAGATCTCAGTGCCAAGGAGGCACAGGATGT12.941176470588235No Hit
ATATTCAATATTGAGAGAATAAAAGAACTGAGAGATCTAATGTCGAAGTC12.941176470588235No Hit
CTATTCAGCAAACCATTTTTAAGTATAAATTAAATTAATGTTCAATTTAT12.941176470588235No Hit
GTGTAAATTGAATACAGTAATGACTTTCTTCCCTTGCAGGATTTTGCATG12.941176470588235No Hit
CCATTGCTTACGGGCTGAAGAGCTGCAGTGATGAGAAGGTGCTGGCGGCT12.941176470588235No Hit
GGGGGGAGGGGGGGGGGTGTGGGGTGTGTGTGGGGGGGGGGGGGGGGGGG12.941176470588235No Hit
TCCTATATGATAGAACCAATGGAACCTCCAAGATCAACATGAAATCCGGA12.941176470588235No Hit
GTTGAATACAGATTTTGCCAGTAAGGTCCTGCACACTTTCCCAATAGAGC12.941176470588235No Hit
CTCTCCCGCTGACTATCAACTGGATCAATCTCCTGGTTGCCTTTCTGAGA12.941176470588235No Hit
ATGCTAGCAAGCATTGACCTGAAGTACTTCAATGAATCAACAAAGAAGCA12.941176470588235No Hit
GTTCCATAGCCTTTGCCGTCGTGCTAGCCAGCACCATTCTGATTTCATGG12.941176470588235No Hit
CCCCAGAACTGTGGGAACACCTGTGCAGGGACACACAGTCAGCCATGGTG12.941176470588235No Hit
GATTAGTATCAAGGTATATAATCTGTTAAAGATTACACTGAAGTTCGCGT12.941176470588235No Hit
TAGTACAACTGTGTAGGATAACTTGAAGTCTGGAACTTGATACCGCCAGA12.941176470588235No Hit
CACCATCTCTGCTTCACTGCAGGAGAAAACAGAGAGGAGACACGGGCCTC12.941176470588235No Hit
CCCAAGACCGAACTTAGGATGTTGACCATGCCGATCTTCATCCCAGGACT12.941176470588235No Hit
ATTTTTCCCATTTGTGTGGCTCCAGTCTCGGGTCAGTGAGTGAGAACTCC12.941176470588235No Hit

[WARN]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)