FastQCFastQC Report
Fri 2 Oct 2020
HTGTLBGXG_n02_S2_R2_D05-FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTGTLBGXG_n02_S2_R2_D05-FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences977615
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTAAACAATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCG16470.16847122844882698No Hit
AATATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCA15850.1621292635649003No Hit
TCCTTAGACAGAATCCAACTGAGGAACAAGCCGTAGACATATGCAAGGCA12440.12724845670330345No Hit
GAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACTCGCGAGATA12280.12561182060422557No Hit
GTCTAAGGATGTCCACCATCCTTACTCCTCCAATCTGTGTGCTGTGGCAC11550.11814466840218286No Hit
ATATAAACACTGCCTGTTCCGCCGGCTACTGGGAGAAACCTTGTTTTACG10990.11241644205541036No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTAGAA5850.055.8118671
CAATTCG5850.055.7347839
GTATACC553.2068416E-452.7675861
AAGTCCG752.5598754E-548.3034749
AGCGAAA1950.044.649491
GTAGAAA10000.042.082151
GACAGTA3300.039.5169837
TTTAAAC9300.038.9544143
TTATACT6050.034.725274
ACAATTC9600.034.7145738
ATAGTGG6700.034.6054763
GAGTTAT1501.1402572E-633.808977
CGAAAGC2603.6379788E-1233.440873
CCTTATA5000.033.358412
GTGGTAT6950.032.3148466
AGGTCGT3850.031.9899376
GGTCGTT3650.031.757947
TATATTC7350.031.5451263
CTTAGAC8100.031.3078083
TAACGGG7000.031.077671145
Produced by FastQC (version 0.11.5)