FastQCFastQC Report
Fri 2 Oct 2020
HTGTLBGXG_n02_S2_R2_D04-FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTGTLBGXG_n02_S2_R2_D04-FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1376741
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA2350.049.3961071
CAATTCG3750.048.2932329
GACAGTA4650.040.5025337
CCTTATA5250.035.914112
GTAGAAA10700.033.9021151
AGTAGAA9050.032.8681951
CGAAAGC3500.031.0795172
ATATTCA6500.027.861484
GTATAGG2105.009133E-727.6061613
GCGAAAG4300.026.9837672
GTCTAAG7300.026.8337571
TATATTC6800.026.6419733
GTTACGC1751.2600473E-424.8455453
TTTAAAC8350.023.4321753
GGCGGTT3102.4419933E-823.3668447
CAGCTCG4103.8198777E-1122.9687339
TTATACT8600.022.7427444
TCTAAGG9350.022.4924832
GGTACTG10000.022.4555385
CAGGTAC9750.022.2972853
Produced by FastQC (version 0.11.5)