FastQCFastQC Report
Fri 2 Oct 2020
HTGTLBGXG_n02_S2_R2_D03_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTGTLBGXG_n02_S2_R2_D03_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTAAGATTAGAGGGGTCGTATAATGTGGTGACCATGTAGATAGTAGAAT13.8461538461538463No Hit
CATAAAGCGCAGGGGCCACTTAATGCCCAGGATCAATGGAAACTAGTCCA13.8461538461538463No Hit
ATGTTCGAGAGTAAAAGAATCAAGATTCGAACACAAATCCCATCAGAAAT13.8461538461538463No Hit
GAGCTGGAGAAGGTGTATGGACCGAAGAACGAAGAAGATGACATGCGGGA13.8461538461538463No Hit
AGCCTAAATTTCTTCCTGATTTGTATGATTACAAAGAGAACCGGTTCATT13.8461538461538463No Hit
CTGTAACACGCAGCCCAAACGAACGGTATTTGACTGTCAGCCCTGGTTAT13.8461538461538463No Hit
CCCAGTGAGCTAGGACTGCAGCGTAGACGCTTTGTCCAACATGCCCGAAA13.8461538461538463No Hit
GTTCAACACATCAGTATCAGTTAGCAAATCAGAACTTGCTTATATAATTA13.8461538461538463No Hit
TGGCTTAAGACAAATGACACTGTAAAATTTGTACAACTTATCCGCGTGCG13.8461538461538463No Hit
TCTCCCTCCATGGTGTCAAGTCGGGCAGTATTGCGAACATTACCATCCCA13.8461538461538463No Hit
AACCGGCGAACTTTCTGACAGCTTGCAATACCAAAAGACAAGATACCAGA13.8461538461538463No Hit
GGAGATTTACACATGTTTGTATCAAGTCTCAGGCTGACCATCTGGTCATA13.8461538461538463No Hit
GATGCACAGAGAGGCAAGTTAAAAAGAAGGCCTATCGCAACACCTGGGAT13.8461538461538463No Hit
CTCTTTCTGTCAAGGCTCCTGGATATCTGTTAATTAGAGATACCAATGGG13.8461538461538463No Hit
GTACCATGGAGTATCTTTCTCCTCGCTTAAGTAACCTCTACATCCAACAC13.8461538461538463No Hit
CACTGAAGCAATCAGCCTCAGGGCGAATAAATAGGATAGGACCTTTAACT13.8461538461538463No Hit
ATGTAGCCTCGTCCCCACAGGAGGTGGCCAAAGAACCGTCCCCAACCAGG13.8461538461538463No Hit
GGACATAACCCCTGCCGTCCTTACCCATTTGTTCAGGCTTGTAGTGGCTC13.8461538461538463No Hit
TCCAAAATTAATCTCCACAATATGCAATTTATTTACTTATCTTATTACAA13.8461538461538463No Hit
GTTTTAAAAATGTGCAAAGAACTTGAAAGATATATCTCAAACCAAGCCAT13.8461538461538463No Hit
ATCCTGTTGCCGACTTCACAAAAACAAATTCATGAGAGCCTATCTGTACA13.8461538461538463No Hit
TATCTGCAATGAATTTGGCAGACGTCTGTGGCCAGGGCACACTGCAGCGA13.8461538461538463No Hit
GCCTATGAGATCCTCCATGGTCTGGACCAGCCTACTTATCTAGTTCATTT13.8461538461538463No Hit
GATCTTCATGATCTCAGAGAACTCTTCTTTCTTGATCCGTCCAGACTCGA13.8461538461538463No Hit
AGCCTAAATTTCTTCCTGATTTGTATGATTACAAAGAGAACCGGGTCATA13.8461538461538463No Hit
CCCCCAGACTCCGCAGCAGGGAAATGCGGAGCTTGGACACAGACCGCCCC13.8461538461538463No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)