FastQCFastQC Report
Fri 2 Oct 2020
HTGTLBGXG_n02_S2_R2_C11-FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTGTLBGXG_n02_S2_R2_C11-FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences771588
Sequences flagged as poor quality0
Sequence length151
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATAACTGTTATCATTTGCCATGCTCCGGGCTCCATGCTGAGTCATTG79441.029565001010902No Hit
CTGTTATCATTTGCCATGCTCCGGGCTCCATGCTGAGTCATTGCAGTTCA42670.5530153397927391No Hit
ATCCTATTGTGAAGGCACCTGAGATTCCTGGGCAAGCGGAAGGGGCAGCA38280.49611969081945284No Hit
CTGTTAGTGTTTCCATCTCCCAGCTGGGGAAACCGAGGCACAGGGAGTTT29260.37921792459188064No Hit
GTTTCTATGTGCAGACATGGGTAGACCGTCTTATCTCTGTTTCCCCATCT28570.3702753282840065No Hit
GTGTATACTCTGCCTGAACGTAATAATAGCAGATATATTGGTGAACAAGG27650.35835186654017426No Hit
GGGTAAGCAGAAGCCAGGGTATCAGCCAGAGACACGCGGGGACTGGTGGG27550.35705583808975777No Hit
GCACAAAGGGCTTGAATGAAAACCAGCTGCAGGGACAGAGAATCCTATTG26790.34720602186659205No Hit
CCCTCAGGCATCTCCTGCTCAGCCACAGTTTCTATGTGCAGACATGGGTA25850.3350233544326765No Hit
TACCTACTCCTACCTGGAGGGAAAGTACTGTTGGTGTACCCATTTTGCAG25460.329968843476052No Hit
AAATAGGGGTGGCGAAGGAACTCGTGTTGCAAGCCAACTCTGATTAACTG25420.32945043209588537No Hit
GTCCAGGACCCTCCCTCAGGCATCTCCTGCTCAGCCACAGTTTCTATGTG25200.326599169504969No Hit
GACATGGGTAGACCGTCTTATCTCTGTTTCCCCATCTGCAAAATGGGTAC24190.31350928215576185No Hit
CCCTCAACATGCTCACCTGGGCGTCCAGGACCCTCCCTCAGGCATCTCCT23640.3063811256784709No Hit
TGGGTAGACCGTCTTATCTCTGTTTCCCCATCTGCAAAATGGGTACACCA23570.30547390576317934No Hit
GGTCGAGCTCATGTGACAATAAGAAGAAGAAAAACAGCAGTAATAACTGT23530.30495549438301267No Hit
GTTTAAAGTGGCTTACCCCAAGTCACACAACGAGTGAGTGGCAGAGCCAG23240.3011970118768047No Hit
GCTAAGTACTGTTAGTGTTTCCATCTCCCAGCTGGGGAAACCGAGGCACA23140.2999009834263882No Hit
GCCATGCTCCGGGCTCCATGCTGAGTCATTGCAGTTCATCTGTCCAGCAG22720.2944576639346387No Hit
GAGTATACACTGGGTATTTTAAAATATGGATGATGGATTGGTATGCGTAT22350.2896623586680975No Hit
ATTGTGAAGGCACCTGAGATTCCTGGGCAAGCGGAAGGGGCAGCATTCTG22280.2887551387528059No Hit
GCTCCGGGCTCCATGCTGAGTCATTGCAGTTCATCTGTCCAGCAGGCTAA21880.2835710249511397No Hit
GTCCAGCAGGCTAAGTACTGTTAGTGTTTCCATCTCCCAGCTGGGGAAAC21820.2827934078808898No Hit
GTCTTATCTCTGTTTCCCCATCTGCAAAATGGGTACACCAACAGTACTTT21820.2827934078808898No Hit
AGCAGGGGGCTTGCAGCCAAGTTTTCTTTTCATTTTTTTCTTTTCAGCAC21550.27929413106476514No Hit
GTCCTAAGCGAGGACTAAGAGAGAATGAAGGCCACTGTCCCAGGTCACCC21170.2743692229531823No Hit
GTACTGTTAGTGTTTCCATCTCCCAGCTGGGGAAACCGAGGCACAGGGAG20890.27074034329201596No Hit
GTGTTGGGCTGGGTAAGCAGAAGCCAGGGTATCAGCCAGAGACACGCGGG18590.24093168893243544No Hit
GTATCAGCCAGAGACACGCGGGGACTGGTGGGCATCTCCTCCCTCTCCCT18470.2393764547919356No Hit
TTTCAGCACAAAGGGCTTGAATGAAAACCAGCTGCAGGGACAGAGAATCC18230.2362659865109359No Hit
GGCTAAGTACTGTTAGTGTTTCCATCTCCCAGCTGGGGAAACCGAGGCAC17710.22952663856876987No Hit
GTGCAGACATGGGTAGACCGTCTTATCTCTGTTTCCCCATCTGCAAAATG17530.22719378735802007No Hit
AAAGCAGGGGGCTTGCAGCCAAGTTTTCTTTTCATTTTTTTCTTTTCAGC17360.22499053899231197No Hit
CATGTGACAATAAGAAGAAGAAAAACAGCAGTAATAACTGTTATCATTTG17260.2236945105418954No Hit
GTACTGTTGGTGTACCCATTTTGCAGATGGGGAAACAGAGATAAGACGGT16950.21967682234560415No Hit
TAATAACTGTTATCATTTGCCATGCTCCGGGCTCCATGCTGAGTCATTGC16790.21760317682493766No Hit
GCAGGGGGCTTGCAGCCAAGTTTTCTTTTCATTTTTTTCTTTTCAGCACA16130.20904938905218848No Hit
CAACATACCTACTCCTACCTGGAGGGAAAGTACTGTTGGTGTACCCATTT15920.2063277293063137No Hit
GGCCCAAGACTGTCTGGCCGAGGAGCCTGCCCTGAGACTGTCACAACATA15800.2047724951658139No Hit
CCCTGAGACTGTCACAACATACCTACTCCTACCTGGAGGGAAAGTACTGT15350.19894036713893945No Hit
GAAGAAAAACAGCAGTAATAACTGTTATCATTTGCCATGCTCCGGGCTCC15280.19803314722364784No Hit
AAAGCAGGTCGAGCTCATGTGACAATAAGAAGAAGAAAAACAGCAGTAAT15030.1947930760976065No Hit
ACCCATGTCTGCACATAGAAACTGTGGCTGAGCAGGAGATGCCTGAGGGA14980.19414506187239822No Hit
GGGTAGACCGTCTTATCTCTGTTTCCCCATCTGCAAAATGGGTACACCAA14790.1916826078166068No Hit
GTCCCAGGTCACCCAGCTAGGAAGCGCTGTCAGGACTTGACGCAGGCCCA14560.18870174238064874No Hit
TCTTATCTCTGTTTCCCCATCTGCAAAATGGGTACACCAACAGTACTTTC14490.18779452246535716No Hit
GCCCAAGACTGTCTGGCCGAGGAGCCTGCCCTGAGACTGTCACAACATAC14300.18533206840956573No Hit
GTATTTAAGACTCTGTTTGTTTGTTCATTTGTTTGTTTTTTAGATTCCAC14170.18364723142402423No Hit
GTATCAGGCCCAAGACTGTCTGGCCGAGGAGCCTGCCCTGAGACTGTCAC14160.18351762857898257No Hit
AGCGAAAGCAGGTCGAGCTCATGTGACAATAAGAAGAAGAAAAACAGCAG14110.1828696143537743No Hit
CTATTGAAGAGCTGAGATCCCAAGGCTCCCACATCCCTGGCCAAGTTGGA14090.182610408663691No Hit
AGCGAAAGCAGGGGGCTTGCAGCCAAGTTTTCTTTTCATTTTTTTCTTTT13970.18105517452319114No Hit
GTCTTGGGCCTGATACTGGCTCTGCCACTCACTCGTTGTGTGACTTGGGG13810.17898152900252465No Hit
ATGCTGAGTCATTGCAGTTCATCTGTCCAGCAGGCTAAGTACTGTTAGTG13770.17846311762235806No Hit
TCTCCTCCCTCTCCCTCAACATGCTCACCTGGGCGTCCAGGACCCTCCCT13670.17716708917194152No Hit
CTCCTGCTCAGCCACAGTTTCTATGTGCAGACATGGGTAGACCGTCTTAT13660.17703748632689986No Hit
GAGTCATTGCAGTTCATCTGTCCAGCAGGCTAAGTACTGTTAGTGTTTCC13590.17613026641160826No Hit
GTCACAACATACCTACTCCTACCTGGAGGGAAAGTACTGTTGGTGTACCC13450.1743158265810251No Hit
GAGTTTAAAGTGGCTTACCCCAAGTCACACAACGAGTGAGTGGCAGAGCC13410.1737974152008585No Hit
GGATATACACAGTTAATGAGTCCTGCCAAGTGCAGAGAAAGCACTTCCCT13160.17055734407481715No Hit
GGTATGTTGTGACAGTCTCAGGGCAGGCTCCTCGGCCAGACAGTCTTGGG13110.16990932984960885No Hit
GAGTAGGTATGTTGTGACAGTCTCAGGGCAGGCTCCTCGGCCAGACAGTC13050.16913171277935896No Hit
GCATTCTGGGTGTTGGGCTGGGTAAGCAGAAGCCAGGGTATCAGCCAGAG13020.16874290424423397No Hit
GTTAATGAGTCCTGCCAAGTGCAGAGAAAGCACTTCCCTATCAATAATCG12960.16796528717398404No Hit
ATTTAAGACTCTGTTTGTTTGTTCATTTGTTTGTTTTTTAGATTCCACAT12890.16705806725869246No Hit
GGCTTACCCCAAGTCACACAACGAGTGAGTGGCAGAGCCAGTATCAGGCC12660.16407720182273441No Hit
GAATCCTATTGTGAAGGCACCTGAGATTCCTGGGCAAGCGGAAGGGGCAG12650.16394759897769276No Hit
GGGCAGCATTCTGGGTGTTGGGCTGGGTAAGCAGAAGCCAGGGTATCAGC12230.15850427948594326No Hit
GTGTATATCCTGCCCAGTCAAAGGCTTTGGCTTCTCCGAGGTAGAGAGGA12200.15811547095081832No Hit
GTCTACCCATGTCTGCACATAGAAACTGTGGCTGAGCAGGAGATGCCTGA12140.1573378538805684No Hit
GCCCTGAGACTGTCACAACATACCTACTCCTACCTGGAGGGAAAGTACTG11980.1552642083599019No Hit
CCCCAAGTCACACAACGAGTGAGTGGCAGAGCCAGTATCAGGCCCAAGAC11970.15513460551486025No Hit
GGCATCTCCTGCTCAGCCACAGTTTCTATGTGCAGACATGGGTAGACCGT11850.1535793713743604No Hit
GAGATAAGACGGTCTACCCATGTCTGCACATAGAAACTGTGGCTGAGCAG11790.1528017543041105No Hit
GTACTTTCCCTCCAGGTAGGAGTAGGTATGTTGTGACAGTCTCAGGGCAG11700.1516353286987356No Hit
GCAGAAGCCAGGGTATCAGCCAGAGACACGCGGGGACTGGTGGGCATCTC11700.1516353286987356No Hit
GTCTCAGGGCAGGCTCCTCGGCCAGACAGTCTTGGGCCTGATACTGGCTC11690.15150572585369396No Hit
GAGAATCCTATTGTGAAGGCACCTGAGATTCCTGGGCAAGCGGAAGGGGC11660.15111691731856897No Hit
CATAGAAACTGTGGCTGAGCAGGAGATGCCTGAGGGAGGGTCCTGGACGC11650.1509873144735273No Hit
CTCCGGGCTCCATGCTGAGTCATTGCAGTTCATCTGTCCAGCAGGCTAAG11390.1476176405024443No Hit
CATGGGTAGACCGTCTTATCTCTGTTTCCCCATCTGCAAAATGGGTACAC11280.1461920092069861No Hit
GCTCCATGCTGAGTCATTGCAGTTCATCTGTCCAGCAGGCTAAGTACTGT11230.14554399498177784No Hit
GCTTAGGACTGTGTATTTTTTATTTTCAAAAGCCTAAAAGCACGTTTGCC11230.14554399498177784No Hit
ATGCTCACCTGGGCGTCCAGGACCCTCCCTCAGGCATCTCCTGCTCAGCC11170.1447663779115279No Hit
GCCAGAGACACGCGGGGACTGGTGGGCATCTCCTCCCTCTCCCTCAACAT10990.14243352670077813No Hit
AAATAAGACGGCCAAGTTTCAGAACAAGAGACACTTAAGGATGGCAAAGA10980.14230392385573648No Hit
ATTTAATACCCTCTCTCTCAGGGAAGTGAAGCCTGGCGAGCCATCCCAGC10730.13906385272969513No Hit
CATTTGCCATGCTCCGGGCTCCATGCTGAGTCATTGCAGTTCATCTGTCC10620.13763822143423693No Hit
CTCCTACCTGGAGGGAAAGTACTGTTGGTGTACCCATTTTGCAGATGGGG10560.136860604363987No Hit
CATGAAAGAGAGTCTTCAAGATATAATATTACAAAGACAAAGCACCCATA10530.13647179582886204No Hit
CCTGAGATTCCTGGGCAAGCGGAAGGGGCAGCATTCTGGGTGTTGGGCTG10320.1337501360829873No Hit
CGCGGGGACTGGTGGGCATCTCCTCCCTCTCCCTCAACATGCTCACCTGG10290.13336132754786234No Hit
GCAGTAATAACTGTTATCATTTGCCATGCTCCGGGCTCCATGCTGAGTCA10230.13258371047761242No Hit
CATCTGTCCAGCAGGCTAAGTACTGTTAGTGTTTCCATCTCCCAGCTGGG10130.13128768202719585No Hit
CACCTGGGCGTCCAGGACCCTCCCTCAGGCATCTCCTGCTCAGCCACAGT10020.12986205073173765No Hit
CAACAGTACTTTCCCTCCAGGTAGGAGTAGGTATGTTGTGACAGTCTCAG10020.12986205073173765No Hit
GTTATCATTTGCCATGCTCCGGGCTCCATGCTGAGTCATTGCAGTTCATC10010.129732447886696No Hit
CTGTTTCCCCATCTGCAAAATGGGTACACCAACAGTACTTTCCCTCCAGG9990.12947324219661271No Hit
ATTCTGGGTGTTGGGCTGGGTAAGCAGAAGCCAGGGTATCAGCCAGAGAC9930.12869562512636276No Hit
GTCTTGAGGGCTGTAAATTGAAGAACTCCCTGAGGCCACAACGAGTTCAG9860.1277884052110712No Hit
CTCCCTCAACATGCTCACCTGGGCGTCCAGGACCCTCCCTCAGGCATCTC9840.1275291995209879No Hit
TGTCTGCACATAGAAACTGTGGCTGAGCAGGAGATGCCTGAGGGAGGGTC9840.1275291995209879No Hit
GATTTATGGAATGGAAACCGCACAGGACTTAGGTTCAAATTCCAACTTCA9820.12726999383090457No Hit
GGGACAGAGAATCCTATTGTGAAGGCACCTGAGATTCCTGGGCAAGCGGA9800.12701078814082126No Hit
CTCTTTCATGAGTGTGTGTTGCTTTTAAAATAATAAAAGACAGCATGGAA9790.1268811852957796No Hit
CACAACGAGTGAGTGGCAGAGCCAGTATCAGGCCCAAGACTGTCTGGCCG9770.1266219796056963No Hit
CCGTAGAAGCTCCTGAAGAATGTCTTTCAGTCTCTGTCTTACTGGACCAC9620.1246779369300715No Hit
GTCACACAACGAGTGAGTGGCAGAGCCAGTATCAGGCCCAAGACTGTCTG9560.12390031985982156No Hit
GTATTAAATGGCTGGCTTTGGAAGCCACGCCACTCCTCTGCTTTGTGCCT9550.1237707170147799No Hit
GTCCAGCACAGAGCTCAATCTCGTGACCTTGAGATCACGGCCTGAACTGA9450.12247468856436336No Hit
TCATAATCCCCTTTATTTCACATTCCTCCTGCCACTGGCAACCACCAGCT9420.1220858800292384No Hit
GGTAAGCAGAAGCCAGGGTATCAGCCAGAGACACGCGGGGACTGGTGGGC9400.1218266743391551No Hit
GAATAAAACTGTCAAAAAATAAAGAAAGTGACTTAGAGCAGGATCCATAA9380.1215674686490718No Hit
ATATATTGGTGAACAAGGCAAACGTGCTTTTAGGCTTTTGAAAATAAAAA9350.12117866011394682No Hit
GGGCTTGAATGAAAACCAGCTGCAGGGACAGAGAATCCTATTGTGAAGGC9160.11871620605815539No Hit
TCCCAGCACAGCAGGGAAATGCTGATCCTCCAAATCCTTCTGGGCTAGCA9150.11858660321311375No Hit
TTCTGGGTGTTGGGCTGGGTAAGCAGAAGCCAGGGTATCAGCCAGAGACA9150.11858660321311375No Hit
CCAATATATCTGCTATTATTACGTTCAGGCAGAGTATACACTGGGTATTT8850.11469851786186411No Hit
CAATAAGAAGAAGAAAAACAGCAGTAATAACTGTTATCATTTGCCATGCT8810.11418010648169749No Hit
TGCTGAGTCATTGCAGTTCATCTGTCCAGCAGGCTAAGTACTGTTAGTGT8670.11236566665111432No Hit
CTTCAATAGTGATGGGGATCCAACCCCACCTACTGCTGATAAAGGAGAGG8630.1118472552709477No Hit
CCCCAACCCCACGCACTTACTGTGAGTTGTTCCTCTACTGGTGTCCCTGA8620.11171765242590605No Hit
GTCATTGCAGTTCATCTGTCCAGCAGGCTAAGTACTGTTAGTGTTTCCAT8600.11145844673582275No Hit
CAGTTCATCTGTCCAGCAGGCTAAGTACTGTTAGTGTTTCCATCTCCCAG8530.11055122682053116No Hit
GCCACAGTTTCTATGTGCAGACATGGGTAGACCGTCTTATCTCTGTTTCC8460.1096440069052396No Hit
GTGCTGGGATGGCTCGCCAGGCTTCACTTCCCTGAGAGAGAGGGTATTAA8360.10834797845482305No Hit
GCCAGTATCAGGCCCAAGACTGTCTGGCCGAGGAGCCTGCCCTGAGACTG8300.10757036138457311No Hit
CCTCTAGGCTGTTGACCCCATGCTGCCCTCCTGCCGTGACTCAGCACCCA8260.1070519500044065No Hit
AATGAAAACCAGCTGCAGGGACAGAGAATCCTATTGTGAAGGCACCTGAG8200.10627433293415656No Hit
ACCTACTCCTACCTGGAGGGAAAGTACTGTTGGTGTACCCATTTTGCAGA8190.10614473008911492No Hit
GGGTACACCAACAGTACTTTCCCTCCAGGTAGGAGTAGGTATGTTGTGAC8180.10601512724407326No Hit
GGCTTGCAGCCAAGTTTTCTTTTCATTTTTTTCTTTTCAGCACAAAGGGC8120.10523751017382334No Hit
CTGCAAAATGGGTACACCAACAGTACTTTCCCTCCAGGTAGGAGTAGGTA8110.1051079073287817No Hit
GTCTGGCCGAGGAGCCTGCCCTGAGACTGTCACAACATACCTACTCCTAC8090.10484870163869836No Hit
TCTCAGGGCAGGCTCCTCGGCCAGACAGTCTTGGGCCTGATACTGGCTCT8070.10458949594861505No Hit
AGGTAGGAGTAGGTATGTTGTGACAGTCTCAGGGCAGGCTCCTCGGCCAG8050.10433029025853176No Hit
GTTCTGCATCTTTGGAATTTAGGGGATCAGGCTGCTATTCTGTGCACAAG7970.10329346749819851No Hit
GATATACACAGTTAATGAGTCCTGCCAAGTGCAGAGAAAGCACTTCCCTA7950.1030342618081152No Hit
CACTTAAGGATGGCAAAGATTGCCAGGCACAAAGCAGAGGAGTGGCGTGG7940.10290465896307356No Hit
TCTCCCAGCTGGGGAAACCGAGGCACAGGGAGTTTAAAGTGGCTTACCCC7770.10070141059736544No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGCGAC100.0070883194145.01526145
AGCGAAA4800.0117.870791
ACTCACG451.09048415E-896.676834145
GCGAAAG6100.092.702642
CCTATAT259.386844E-486.9978562
CGAAAGC6500.086.913263
ATATCAC259.4280485E-486.901995
CTATATC300.001943248172.427723
TACCTAT801.0004442E-1072.4183357
CTGCCCG955.456968E-1268.69143145
ACCTATC851.70985E-1068.162848
GAGCGAA754.834874E-967.700141
GTGGCGA6800.067.09789
AATAGGG6850.066.677192
TAGGGGT6900.066.121084
GACGACT554.0581854E-665.91603145
ATAGGGG6950.065.653913
AAATAGG7100.064.362811
TCACTTG350.003581233662.0768788
TAGCACT350.003582153262.0728534
Produced by FastQC (version 0.11.5)