FastQCFastQC Report
Fri 2 Oct 2020
HTGTLBGXG_n02_S2_R2_C10_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTGTLBGXG_n02_S2_R2_C10_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1542865
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA3700.060.744511
CAATTCG3650.043.6485529
GCGAAAG6950.034.4061972
CGAAAGC6550.033.1745033
GTATTAC8700.032.5006751
CCTTATA9650.030.0358722
ATAGACC8800.028.8077813
TTATACT14350.024.7293284
TATAGAC14400.024.657052
TAGTACG3001.7284037E-824.1405354
TATACTG16250.024.0670365
ATTAACG1851.8431501E-423.4911353
GTCCATA5300.023.2552011
GTAATAC9850.022.7955423
GTATGCT13700.022.226741
ACCTATC8900.021.9698898
TATTACA13600.021.845022
TACCTAT9000.020.9204437
ATTACAT13900.019.801283
CTTAGAC6950.019.801283
Produced by FastQC (version 0.11.5)