FastQCFastQC Report
Fri 2 Oct 2020
HTGTLBGXG_n02_S2_R2_C10-FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTGTLBGXG_n02_S2_R2_C10-FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1432185
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTAAACAATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCG33410.23327991844629012No Hit
GAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACTCGCGAGATA20270.14153199481910508No Hit
GTAGAAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATCC19880.13880888293062696No Hit
AATATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCA18160.12679926126862104No Hit
AGTAGAAACAAGGCATTTTTTCATGAAGGACAAGTTAAATTCATTATTTT16420.11464999284310337No Hit
GGTCAAATATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATG16100.11241564462691621No Hit
AAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATCCGAAT15070.1052238363060638No Hit
CACTAAGACCACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAG14900.10403683881621438No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA5100.064.000411
TATACCG651.1008491E-555.7095075
ACCGTAG400.0060771254.3167768
TACCGTA657.3752063E-444.5691647
GGTCGTT6700.037.8338977
CGAAAGC10400.034.8269583
GTACGAT850.002753519534.0894433
CAATTCG13300.033.217439
AGGTCGT7750.032.7068756
GCGAAAG11900.031.066362
GTATACG2003.2427306E-729.013521
GTAGAAA18050.028.933151
CTATACT4800.028.6691864
AGTAGAA13450.028.5820921
AGCGTAA1606.8801615E-527.1583886
ATATTCA12750.026.6987364
TCAATAT13050.026.083158
ACAATTC18350.026.0483688
TATACTG12050.025.8436645
TATATTC13400.025.4080833
Produced by FastQC (version 0.11.5)