FastQCFastQC Report
Fri 2 Oct 2020
HTGTLBGXG_n02_S2_R2_C08_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTGTLBGXG_n02_S2_R2_C08_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1413936
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTAAACAATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCG33110.2341690147220242No Hit
GAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACTCGCGAGATA20040.14173201615914724No Hit
GTAGAAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATCC19710.13939810571341277No Hit
AATATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCA18070.1277992780437021No Hit
AGTAGAAACAAGGCATTTTTTCATGAAGGACAAGTTAAATTCATTATTTT16340.11556392934333662No Hit
GGTCAAATATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATG15960.11287639610279389No Hit
AAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATCCGAAT14970.10587466476559051No Hit
CACTAAGACCACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAG14770.10446017358635752No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA4300.074.238211
CTAGTAC2800.038.8074653
AGTAGAA14800.037.7461131
GTATTAG5450.035.942681
CGAAAGC10050.033.8776933
CAATTCG14000.033.1075029
GGTCGTT6550.030.9582127
AGCGTAT950.00472480330.53047145
GTTACGC1952.6273847E-729.719223
GCGAAAG11650.028.6203672
CAGTATG6450.028.0707999
TATATTC11700.027.8617673
GTAGAAA20900.027.7707081
TAGAAAC22100.026.8784123
TGTATAC2705.5806595E-926.8459992
GTATTAA6600.026.3821721
ACAATTC18500.026.2278218
TAGTAGA3201.2460077E-924.9164412
AGGTCGT8500.024.7089086
TTAAACA24000.024.4443784
Produced by FastQC (version 0.11.5)