FastQCFastQC Report
Fri 2 Oct 2020
HTGTLBGXG_n02_S2_R2_C08-FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTGTLBGXG_n02_S2_R2_C08-FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1578822
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA3900.081.851011
CAATTCG4400.057.6168149
GCGAAAG6000.053.172792
CGAAAGC6600.047.2058643
CCTTATA9500.033.5828172
GTATTAC8450.030.9087751
TAGACCG1204.082049E-430.1792765
TTATACT14300.028.3660954
TATACTG16400.027.823825
TATAACG1050.00770544227.6222322
GTATTAG4002.7284841E-1123.5786761
TAGACAG9250.022.7078675
TAGCGTG1650.002616095121.9485665
ACAATTC14000.021.7290788
ATTCGAC5550.020.9152471
ACCTATC8700.020.8132958
GTATAAG11550.020.7285041
GTAATAC10450.020.1072713
GAACCGC2205.8983313E-419.77626145
AGTAGAA11850.019.5914971
Produced by FastQC (version 0.11.5)