FastQCFastQC Report
Fri 2 Oct 2020
HTGTLBGXG_n02_S2_R2_C06-FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTGTLBGXG_n02_S2_R2_C06-FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1610661
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAGACATCTTAACAATATTGTTCTTCCAATCCATGAGCATGGATTGT19800.12293089607310291No Hit
GTATTACATCAAATATGTAGATTTTGGGTAGTATAGACATCTTAACAATA18750.11641183340255956No Hit
GTTTATATGAGAAAGGAGGGGAATGAAAGAGAAAATAGAATCAAAACACC17920.11125866957727294No Hit
ATGTAATACCTATCAAAATACCAATAACACTTCTCACAGAACTAGAACAA17080.10604341944083827No Hit
TTTTTAAACAATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCG16460.10219406814966028No Hit
CCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATACA16260.10095234192669966No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA3350.067.142321
CGAAAGC4700.046.252583
GCGAAAG5100.044.0703932
GTATTAC13400.043.3176231
CAATTCG5950.042.607729
AGTAGAA10900.039.2739831
GGTCGTT4150.038.39597
AGGTCGT4550.035.022616
TATTACA17550.033.462882
TACATCA15950.032.6970835
CCTTATA9100.032.6661452
TAACGAC6100.032.073513
ACATCAA16250.031.6477036
GACAGTA5400.030.849237
TCGTTTT4950.030.7292049
CTTAACG6350.029.7083871
ACGACTG6350.029.6576585
GTAGAAA14500.029.5011832
AACCTCG1306.476093E-427.890936145
TATATTC10350.026.6045423
Produced by FastQC (version 0.11.5)