FastQCFastQC Report
Fri 2 Oct 2020
HTGTLBGXG_n02_S2_R2_C05_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTGTLBGXG_n02_S2_R2_C05_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGTAATCGAATAAAAGAACAAAGAGGAAATAGACATAACAACCCACTTT12.941176470588235No Hit
CTTGTGTTGAGAATAGAACGATTAGTCTTGGGAATCCAGGAATGTGTAGT12.941176470588235No Hit
GTGCAAAGGACGCACAGGAAGTGATTATGGAAGTTATTAGCCCAAATGAA12.941176470588235No Hit
GCTTCATATAGCCCCCCAAGATCGAAGGTGCCAGGTTCCAGGTTGTCCCT12.941176470588235No Hit
GGGTTTGCACTTGTCTGTCAAGGTCAGTGGTATGTCTTGCTTGTGTTCCT12.941176470588235No Hit
ATCCATCATTGCCCTCTGGGCAGCTGTTTGAAATTTTCCTTTGAGGAGAT12.941176470588235No Hit
CAACATAACCTTATACTGGAGATCCTCCATACAGCCATGGAACAATCGAG12.941176470588235No Hit
GTATTTGTCTATGCATATGTACTCTTGGACTATCGCAGTAGTAAACAGCC12.941176470588235No Hit
GTGGTAATGCAAGCCCATGGTCAAGCCAAAAGCATGAAAAATGAAGCCCT12.941176470588235No Hit
TCTATATATGGGTCATCCTGTTTTCTTAGGGTCCTTCCAAGCACTGGGAT12.941176470588235No Hit
GTGCTGGCCAGGGCGCCCCCAGATGGGAGGGTGGAAGGAGGGGCTGTTTG12.941176470588235No Hit
GTCCAGACTCGAAGTCGACACTGGCAACAATCCGGGCCATAGACACCAGG12.941176470588235No Hit
GTATGGATAGCAAATAGTAGCATTGCCACAACTACTTCAGTGCATGTGGG12.941176470588235No Hit
CTCAAACCCCCCACCAACCCCTCCACCCTGGCTAAGCCAACAAGCACACC12.941176470588235No Hit
CTTGCGATATGTTCAATCGGGGCAACATCTTCTCCTATTTCATCAAGTTC12.941176470588235No Hit
GCCTTATCGTTTGTTTTGCTCCAGAGGGTTTGTCCTTGTTCATTCCTCTC12.941176470588235No Hit
ATCTCACAAAACTCTTCTGTCTTCAACCGTCCACAATCGAAGTCGACCAT12.941176470588235No Hit
ATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCC12.941176470588235No Hit
CTCTTCTTTCTTGATACGTCCAGACTCGAACTCGACCCAGGCATCAAAAC12.941176470588235No Hit
CCCTAAGGCGGTGAGGAGGGGGAAGGACCTTGACCCACACAGAAAGTGAA12.941176470588235No Hit
AAAGAGAACATGCACATCATACATTCAAAGTAAAAATTCGTTTATTAACA12.941176470588235No Hit
GGGAGAGGGAGAAGAAGTCTTACCGCTGAGAATAGAGACGTAAATTGTGA12.941176470588235No Hit
ATTCGACACTAATTGATGGCCATCCGAATTATTTTGGTCGCTGTATGGCT12.941176470588235No Hit
CCTCAAATTTCTGTCCCAATTGCTCTCGCCACTTTTCATTTCTGCTCTGG12.941176470588235No Hit
CTGCTCTTCCTGTTGATATTCTTCCCTCATGGACTCACGCACTCCTTCCG12.941176470588235No Hit
CAACAAGTTAGTGGTTCACTCATTAAACGTGAAATTCCGAATTTTTTCAG12.941176470588235No Hit
GTTACTTTACAAAATAAAAAGTCCCAATACTACCATAAACAATTGTACCC12.941176470588235No Hit
CGGCAGTCTGGTGTGCTGCACACGGACAGCTGGATGCAGAGCTTCTAGTA12.941176470588235No Hit
ATATTTGAGAATTCATGCCTTGAAACAATGGAAGTTGTTCAACAAACAAG12.941176470588235No Hit
ACAAGTTATAAAATATCACTCACACCTAGAAATATGCATATAGCCCACCA12.941176470588235No Hit
GCACACAGATTGGAGGACTAATCATGGAGGACAACCTTAGCGAGAATCCA12.941176470588235No Hit
TTGTTTAATGTATTAGGATTTCCATATAAAAGACTAAAGGGGTGAATCAA12.941176470588235No Hit
GTCAAGCGCAAACTGCACGAATACCAAAATGACATTATACGTGCTATCAT12.941176470588235No Hit
TTTTTAAACAATTTGACAGGAATTCATGTGCTTCGGGAGTCTTTTGGTCG12.941176470588235No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)