FastQCFastQC Report
Fri 2 Oct 2020
HTGTLBGXG_n02_S2_R2_C04_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTGTLBGXG_n02_S2_R2_C04_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences97
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTTTTGCCAGTGACCCTGGCGGAGGGCAAGGGTTCTGTCCTAGTTGAAT11.0309278350515463No Hit
TTTAAAATTAACCCATAAAGGTTTCTGTTCAGTCTAACTTCTTTGGTCCT11.0309278350515463No Hit
CAATAAGGCATGTGAATTGACTGATTCAAGCTGGATAGAACTTGATGAAA11.0309278350515463No Hit
GTTTCTTCTAGAGAGTTAGCGGGCAGCCGGGCGTTGTGGAATTGGATTAG11.0309278350515463No Hit
GGGGTTTAGCCGCATGATCATCCGGGGTTTCATGGAGGGGGGTGTCGGGT11.0309278350515463No Hit
GGCTGGAACCCTGATGTGCAGGGACAGTCCGAGCTGCTTCCTGGGGAAAG11.0309278350515463No Hit
CATTATTTTTGCCGTCTGAGTTCTTCAATGGTGGAGCAGCTCTTCATGCT11.0309278350515463No Hit
GCCCATCTCCGCGGAAGAACCCTGCGCCCTGCCTCGCCAGGTGCCTTCGG11.0309278350515463No Hit
GGCCCCGGGTTGCCAGGGGCGTCGGGGCCGCCGGCGCGGCGCGGGCCGGC11.0309278350515463No Hit
GTTTGGATCAATCATCACCAATGGGATGCCGTACCCGTGAATTTTGTAAG11.0309278350515463No Hit
CATCACTGTCATACTGTTTAAGGTCTCCAACATCTTTGCAGTCATCAAAG11.0309278350515463No Hit
GCCTGTTCACTCGATCCAGCCATCTGTTCCATAGCCTTTGCCGTAGTGCT11.0309278350515463No Hit
TGGGGAGGGAGTTCTTCAGGATGGGCGGGCGTGGAGTCTTGGGGGGGTCG11.0309278350515463No Hit
ATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCC11.0309278350515463No Hit
GGGGGGGGCGGGGGGGCGGGGGGTGAGCGGGGGGCGGGGGGGCGGGGGCG11.0309278350515463No Hit
ATCAAAGACTACAGATACACATATAGGTGCCATAGGGGAGACACACAAAT11.0309278350515463No Hit
GTGTGTGCCACTTGTGAACAGATTGCTGATTCACAGCATCGGTCTCACAG11.0309278350515463No Hit
ACCCACTGTCCTGTACTGCCTCTTCGCTTTCTTTGTGCCTTTGTTAGGTG11.0309278350515463No Hit
TTGCAAGAGAGGGAAGTGGTTCGATGTCGATTTTGTTGTATTAGCTTGTG11.0309278350515463No Hit
CATTCTTTGGCTGGAAAGAGCCTAACATAGTCAAACCACATGAGAAAGGC11.0309278350515463No Hit
GTCTTCAATACTTAATTTCAGAGCATCCATCAGCAGGAACTTTGACCGCT11.0309278350515463No Hit
GCTCAATGCATCCTGTGCCGCCATGGATGACTTTCAGCGGCTCCCAATGA11.0309278350515463No Hit
AAAGTACAAATCAGGAAGGCAAGAGAAGACCCCCGCCCTCAGATTGATGT11.0309278350515463No Hit
CTCTTTGACAGAAGACTCGGACTCAATCATGCTCTCAATCTGCTGAAGAG11.0309278350515463No Hit
GCTTATGGTCCAGCCCCTGTTCGCTCTCGTGAAGTGTCCATGGGGTCTGG11.0309278350515463No Hit
GTCTACGGCTTGTTCCTCAGTTGGATTCTGTCTAAGGATGTCCACCATCC11.0309278350515463No Hit
CTCAAATCTTGAGTTGTATGGAGAGGGAACTTCACCAATAGGACAGCTCA11.0309278350515463No Hit
GAACATATCGCAAGCATGAGGAGGAACTATTTTACAGCAGAAGTGTCCCA11.0309278350515463No Hit
GATTCCAACTGAATAGGTCAATTGTAGCAAGGCTCTCCGACTTAAAGTAG11.0309278350515463No Hit
GCTGGATCTGGTATTATCATTTCAGATACACCAGTCCACGATTGCATTAC11.0309278350515463No Hit
GACCAGGACCAGCAGCTGGCGAGTCACTGCTGATGGCAAAGGAGACCCCG11.0309278350515463No Hit
TCCTTAGACAGAATCCAACTGAGGAACAAGCCGTAGACATATGCAAGGCA11.0309278350515463No Hit
AAAGTGGGTTGTTATCTCTTTGTCCTCGTTGTGGAGTGCGTCCGGTGCGT11.0309278350515463No Hit
GAATTAAACCCTACTTTGATAACATCACTAAAATTGCAATAAATAATAAT11.0309278350515463No Hit
ATATAAAACTTATTTCGAAAAGGTCGAAAGGTTGAAACATGGTACCTTCG11.0309278350515463No Hit
CTCTTATTTTCAGTGTTTGGAGGTTGCCCGTTAGCACTTCTTCTTCTTTC11.0309278350515463No Hit
GAATGAAAAGATGTGAATGTGTGTCTTCTCAGAGTTTAGTTTGTTGGCTT11.0309278350515463No Hit
CGATTCTGTTGGTCGCTGTCTGGCTGTCAGTAAGTATGCTAGAGTCCCGT11.0309278350515463No Hit
CTGTTCATCCTCAAGAATTCCCCTTTGGCTTGTGTTGAGAATAGAACGAT11.0309278350515463No Hit
CTTCAACTCAGTAATTGAGTTATCTTTTATTGCTTATTTGGTCACCACTT11.0309278350515463No Hit
ATGTCCTGTAGCTCTGCTAGCACCTGCTTCCAAGCCATGAGGTAATTGGG11.0309278350515463No Hit
CTATAAGACAAGAAATGGCCAGTAGGAGTCTATGGGATTCCTTTCGTCAG11.0309278350515463No Hit
AAGCAGGAGTGGACAGATTCTACAGGACCTGCAAGTTAGTGGGAATCAAC11.0309278350515463No Hit
GATGGCAATGAGATACCCAATTACAGCAGACAAGAGAATAATGGACATGA11.0309278350515463No Hit
GTCTATTGTCCCTAAGGCAACAAGAAGCCGGTAAAGTGGAGTCGTAAGGA11.0309278350515463No Hit
GGGAGAAACCTTGTTTTACGGACCAATTCTCTTTCTAGCATGTACGCCAC11.0309278350515463No Hit
CCCAATTACAGCAGACAAGAGAATCACATCTGGGGTGGAGTCTGCTGTCC11.0309278350515463No Hit
CATGGCGACTACCACCAATCGACTAATCAGGATTGATACCAGAATGGTGG11.0309278350515463No Hit
ATCTAATGTCGCAGTCCCGCACTCGCGAGATACTCACTAAGACCACTGTG11.0309278350515463No Hit
CTTCGGCCCTGGCCCCTGCAGAAATCAAGTGAACATCAGGCGGAGAGTTG11.0309278350515463No Hit
ATAACAGACACTATCAAGAGTTGGAGAAACAATATATTGAGAACACAAGA11.0309278350515463No Hit
GTTCTTTGCGTGACCATCGTCTGGGTCATGGTGTCTCTTGCTCTCCTTTT11.0309278350515463No Hit
GGATCAGACCGAGTGATGGTATCACCTCTGGCCGTAACATGGTGGAATAG11.0309278350515463No Hit
ATAGTCGAATTATTTCTTACATTGTCTGTTCCGGGAAATCTCTAGGGTCA11.0309278350515463No Hit
CTGCCCGACATGACCCCAAGCACGGAGATGTCGCTGAGAGGGATAAGAGT11.0309278350515463No Hit
GGGGTTGGGTGTTCGGCGGAGTGGGGTGTGCGGGTGGGTGTGTGGGTGGT11.0309278350515463No Hit
ATATAAACACTGCCTGTTCCGCCGGCTACTGGGAGAAACCTTGTTTTACG11.0309278350515463No Hit
TCCTAATACTGTAACACTTGAAGAGATGGATCCCCATTAGTAATCTTTAC11.0309278350515463No Hit
CTCAAAATATACAGAGATTCGCTTGGAGAAACTGTGATGAGAATGCGAGA11.0309278350515463No Hit
CTGCTATGTTTGTTTAGTCTTTATTCATCCAGTAGATAATTTTATTGAGT11.0309278350515463No Hit
GTGCTTGTGTCAGGAAGGAGTTGAACAAAGATGCATTAAGCAAAACCCAG11.0309278350515463No Hit
CGATTCTGTCTAAGGATGTCCACCATCCTTACTACACCAATCTGTGTCCT11.0309278350515463No Hit
ATCAATCTCTTTATGACTGACAAAGGGATTCAGGGGATTACAAAGTCTTC11.0309278350515463No Hit
CCTCAAGAGCATGTCTCCTATTTCAAGAACACAGTATTTTTCCCATTTGT11.0309278350515463No Hit
GATTGAGAGCATGATTGAGGCCGAGTCTTCTGTCAAAGAGAAAGACATGA11.0309278350515463No Hit
GATCACACAGCGTCTGTCACTCAAAGGCTCTTATCGTCTGAATTTGTGTG11.0309278350515463No Hit
GAGCTGGCGAGAGAGAGAGGACCCCGACACCGCATTCGTCATAAAGGGCA11.0309278350515463No Hit
TCCTTACTCCTCCAATCTGTGTGCTGTGGCACATTTCCAAGAGAGATGCT11.0309278350515463No Hit
GCACAGGGTAAACTGGATTCTGAGGTGGTTTTGCATAAATAAAGGGCAAC11.0309278350515463No Hit
GGGTCGTGAGAAGAGAGCATAAGGCGGCGCGCGTGAGCAGGGGCCCCCCT11.0309278350515463No Hit
GGGGTATTCAGCGTTAAGGGACCTAACATAGACACACCAGCCNNNNNNNN11.0309278350515463No Hit
GTGCATTGATGATGATGTAGTGACATTATGCTGCGGCGTAGCGGGCGCAT11.0309278350515463No Hit
GAGGTGGATTGTGGGCACAAGGGAGCTTAATGTGGGCCAGTGCCCATGGA11.0309278350515463No Hit
CCTAGACACCATGGAATCCACCAAGCTAGAAATTACAACCGGTCTCCTAG11.0309278350515463No Hit
AACCTGATCCTCTAACATTCACATTAAATGATTAAAATGGCATAATAATA11.0309278350515463No Hit
CTTGGGTCGCGGCATCTGCACTCCCCTTCGCGTCTGGTCGGGCGGGTTCT11.0309278350515463No Hit
TGTCAGGAGTGAGGCCCACTGGGGTCAAGGAACCAAAGGAGGGATAAGGG11.0309278350515463No Hit
AAAGCAGGAGGTCAAGGGGAAAGGCTGGCCTGTAGGGAACAAGGGAGGGA11.0309278350515463No Hit
GTGCAGACCCTGGTGTACATGTGCGGCGATATGCTCAGCAACGTCGGGGC11.0309278350515463No Hit
GCGGAAAACGCAAAGAAAGAATATTGCGAAGATCCCAAAATCTAAACTAA11.0309278350515463No Hit
GGTGGAACAGATCTTCATGATCTCAGAGAACTCTTCTTTCTTGATCCGTC11.0309278350515463No Hit
ATTTCAGTCTCTTGTCCCTAAGGCAACCAGAAGCCGGTACAGTGGATTCG11.0309278350515463No Hit
GTGTATCTGTAGTCTTTGATGAACAATTGAAGAGCCATCTGGGCCGTTGC11.0309278350515463No Hit
CTCCACCCCAGATGTGCCGTCCTCTGGAGCTTCAGTCAATGCACAGGCAT11.0309278350515463No Hit
ACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGA11.0309278350515463No Hit
GGAGTAGGGATGGTTAGTGATTATGGATCACATTTCTTGACTATAATTGT11.0309278350515463No Hit
CAGTGGTGAACAGTATATGTAAAAAAACAGGTGTAGAGAAGCCTAAATTT11.0309278350515463No Hit
TGTGTGTCTTCTCAGATTTTATTTTGTTGGCTTTCTCTAGGTAATATATG11.0309278350515463No Hit
CTCCCAGCCTGCCTGACCTGGGCTGGATGTCCCCCCTAAACCTCTCTGGG11.0309278350515463No Hit
CTGTATAAGTTAGCGACGGAAGAGTAGTTATTGAAGACATAATAGGGATA11.0309278350515463No Hit
CCCTATGGCACTCCATCCACACTACATGGCCCAGGGAAGAGACATCAGAC11.0309278350515463No Hit
TAGTTGAATTGCAATTGAAATATAAAGCCACCACTACAATTATGAGTTAA11.0309278350515463No Hit
TACCCATACGTCAAACAGCAATAAGGCAAATAGTATAATCATTATTCCAA11.0309278350515463No Hit
CTTGACGAAGAGAGCAGGGCAAGAATCAAAACTAGGCTTTTCACTATAAG11.0309278350515463No Hit
GTGGGGGGCGTTGGGGTGGGGGGGGGGGGTCCCGGGGGGGCTGGGTGGGA11.0309278350515463No Hit
GATGTATTATGCCCCAATACTCAGCAGCAGGGAACTGAGGCTCTTGGCAA11.0309278350515463No Hit
GTATGGAAATGTGGCGCTGCCTGCGTCAGTCACTTCAGGAGCATGCGAGC11.0309278350515463No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAAGTT50.0145.05
TAAGTTA50.0145.06
ACGTTGT50.0145.0145
TATAAGT50.0145.04
GTATAAG50.0145.03
GTTAGCG50.0145.09
TGTATAA50.0145.02
CTGTATA50.0145.01
AAGTTAG50.0145.07
AGTTAGC50.0145.08
Produced by FastQC (version 0.11.5)