FastQCFastQC Report
Fri 2 Oct 2020
HTGTLBGXG_n02_S2_R2_C03_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTGTLBGXG_n02_S2_R2_C03_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences42
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCCTCTATCTCTGCTCAGAGCTGCATGCAAGCCTAGAACTAATTGTTAT12.380952380952381No Hit
CAAAGAAGACAAAGGCAAAGATTGATGCAAGGAAAGACTAACATAATAGA12.380952380952381No Hit
CATACTGTCCATGGCTTGGATTAGAGTGACACTACTAGGTTTGATTCGTG12.380952380952381No Hit
GACAATACCGTATGCTTCATCAATGATGAGGCAGATCAATGGCCCTCAGT12.380952380952381No Hit
ATGTTATACAACAAAATTGAAGTTGAACCATTTCAGTCTCTTGTCCCTAA12.380952380952381No Hit
CTAGTCCAGATTGTGTTCTCTTTGGGTCGCCCTCTGATTAGTTAAACCAA12.380952380952381No Hit
GTATGGCCTGGATCACTGGCCTGGTTTGCTTTCTGAGAATAGCTGTTGCT12.380952380952381No Hit
CAATAAGGCATGTGAATTGACTGATTCAAGCTGGATAGAACTTGATGAAA12.380952380952381No Hit
AGTCTCTTGTCCCTAAGGCAACCAGAAGCCGGTACAGTGGATTCGTAAGG12.380952380952381No Hit
ATCGAACAGATGGCTGGATCGAGTGAACAGGCAGCGGAGGCCAGGGAGGT12.380952380952381No Hit
GCTGGAAAGAGCCTAACATAGTCAAACCACATGAGAAAGGCATAAATCCC12.380952380952381No Hit
AGTTTGCTGCAATATGCACACATTTGGAAGTTTGTTTCATGTATTCGGAT12.380952380952381No Hit
GAAACAAGGTAGTTCTTTACTCTAGCTCTATGTTGACAAAATGACCATCG12.380952380952381No Hit
AAAAAACATCTTTTCATTCACAGGATAGCAGAAGGCCACCAAAAAGCACT12.380952380952381No Hit
ACCGGGGGCAGCTACTCCTCGTGGGAGCATGTCCTGTGATTTTGTCTGTC12.380952380952381No Hit
TTCCTATATGTGAGAAAAAATGGAACATCCAATATAAAGATGAAATGGGG12.380952380952381No Hit
TGATGCCACATATCAGAGAACACGAGCGATTGTTCGCCCAGGAATGGATA12.380952380952381No Hit
CTATAGCGAATACCAAATCAACCTCGAACCCCGAGATACATAATAACTAA12.380952380952381No Hit
CCATGAGGGAATACAACCAGCACTGGACAGATTCTACAGGACCTGCAAGT12.380952380952381No Hit
GTGTATGGGCTTGCAGTAGAAACTGGGCATGACTTTGATAGGGAATGGTA12.380952380952381No Hit
ATATGGATTAGCCACTCAATGCAACTTGGGAATCAATATCAGAATGAAGC12.380952380952381No Hit
GACTGAATAAGAGAGGCTATCTAATAAGAGCACTGACATTAAATACGATG12.380952380952381No Hit
GTATGGATAGCAAATAGTAGCATTGCCACAACTACTTCAGTGCATGTGTG12.380952380952381No Hit
CTCTAAATCTGCATAAGACACTGAACAAGGCAGCGCCTCATATACATGCC12.380952380952381No Hit
CCAGATGGCTCTTCAATTGTTCATCAAAGACTACAGATACACATATAGGT12.380952380952381No Hit
ACCTATATGTGGATATGTAGTATTTGCTGAACAATTGAAGAGCCATCTGG12.380952380952381No Hit
GTCTCGGGTCACTGAGTGAGAACTCCATACTTACAAAGTTCACCACATCA12.380952380952381No Hit
CATTAAAGCTGGGCCATATATCTTTTCTTATTTGCAAAAAATGAGAAATC12.380952380952381No Hit
CATATAGACTGTTGAATACAGATTTTGCCAGTAAGGTCCTGCACACTATC12.380952380952381No Hit
ATACGAAAACACTAATCAGCATCAAAAGACTTGACAGAAACAAGATATTT12.380952380952381No Hit
CAATTAGAAAATTAGCCTTCTATCCTAATGCAAGATTGCTAAGTTCATTG12.380952380952381No Hit
CACATATACATCACATCCTACAAAAAGCCATTGAAAACGAAAATGATACA12.380952380952381No Hit
CGAGTCTTCTGTCAAAGAGAAAGACATGACCAAGGAATTCTTTGAAAACA12.380952380952381No Hit
GTCTTTGTTTTTTCTTCCCTATTGTTCTTTGAGTGACCCTATTCTTGGTA12.380952380952381No Hit
CAACAATGCAGTGAATCACCAATCAGTTCACAAGTGGCACACACTAGACC12.380952380952381No Hit
ATCCGAAAGCCATTTGCAGACAATGGAATCGGTGATGCCCCATTCCTTGA12.380952380952381No Hit
AGCAAGGAACCATCGCCTTCTTTGCCTTTTATAACGAGAGCTCCAATACA12.380952380952381No Hit
ATTCGCCACTAATTGATGGCCATCAGAATTCTTTTGGTCGCTGTCTGGCT12.380952380952381No Hit
GCCACATTGTATTGGCCAGAAATTCCAATTGTTTGCCAATTTATGCGATC12.380952380952381No Hit
CAATAATACCACACAGGTTAATATTGCCATAATCAAAAACTACCCCTCAG12.380952380952381No Hit
GCCTAAATTTCTTCCTGATTTGTATGATTACAAAGAGACCCGGTACATTG12.380952380952381No Hit
CCAACATAATTGATTACTAATAAGTTATTTTCAACTTCTAATTTCCTGGA12.380952380952381No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)