FastQCFastQC Report
Fri 2 Oct 2020
HTGTLBGXG_n02_S2_R2_C01-FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTGTLBGXG_n02_S2_R2_C01-FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1666119
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTATATACAGCTCGCTGAAGAGGTCCATGACCTCAAAAAAGATCAAATG30490.18300013384398112No Hit
CATATATACTTACACAGTTTGAGCATTCACAGGCCACTAGAAGCCCATCT23100.13864555893066463No Hit
GTATATACATACATATATACTTACACAGTTTGAGCATTCACAGGCCACTA19920.11955928718176793No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA4400.064.322191
CAGCTCG14500.046.465789
CGAAAGC6900.040.962863
GCGAAAG8600.035.421322
GTATAGA5100.034.149941
TATATAC32850.032.430733
TATACAG24000.031.0898195
TAGTGCG1204.0774557E-430.18527
TACAGCT22950.029.6722477
ATACAGC24900.027.9287246
ACAGCTC26000.027.5854558
GCTATAT3555.456968E-1226.5744271
TATACTT12950.026.29185
CATACAT12300.025.9158748
GTCTTAT10700.025.7720341
GTATATA28500.025.7173041
ATATACA30500.025.4172344
CATATAT16750.023.8285061
TGTATAT29450.023.162821
TATATAT16400.022.9971182
Produced by FastQC (version 0.11.5)