FastQCFastQC Report
Fri 2 Oct 2020
HTGTLBGXG_n02_S2_R2_B12_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTGTLBGXG_n02_S2_R2_B12_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences41
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGGGAATCAATTCGCCACTAATTGATGGCCATCCGATTTCTTTTGGTCG12.4390243902439024No Hit
TCATTAATCTGGCACTCCTCGTGTGCTTCATATCGCCCCCCATGATCGCC12.4390243902439024No Hit
NNNNATGGATTCGAGCCGAACGGCTGCATTGAGGGCAAGCTTTCCCAAAT12.4390243902439024No Hit
ATATGTAACAAAACAGGGGTAGAGAAGCCTAAATTTCTTACGTATTTTTA12.4390243902439024No Hit
CTGAAGTTCGCTTTCAGGACTATGGTCTTTTCCATGATCGCCTGGTCCAC12.4390243902439024No Hit
GTCCAGCCTCGCAGTCGGCCCGGGCATGACTCCGGGCCCTAGACACCGTG12.4390243902439024No Hit
CTTCTGGTACATCTGTTAATCCTCAAGAATTAGCCTTTGGCTTGTGTTGA12.4390243902439024No Hit
GTATGGATAGCAAATAGTAGCATTGCCACATCTTCTTCAGGGCATGTGTG12.4390243902439024No Hit
CCTAATTCCAGCGCAAAATGCCAAAAGCACCACATTCCCTTATACTGGAG12.4390243902439024No Hit
GTTCATTGATGCTTAATGCTGGGCCATATCTCTTGTCTTCTTTGCCCAAA12.4390243902439024No Hit
GAGTACATGAAGGGTATGAAGAATTCACAATGGTTGGGAGAAGAGCAACA12.4390243902439024No Hit
GTCAATGCACCTGCATCCTTTCCAAGAACTGTAAGTCGTTTGGTTGCCTT12.4390243902439024No Hit
CAGTATGGATAGCAAATAGGAGCATTGCCGCAACTGCGTCAGTGGATGTG12.4390243902439024No Hit
ACCCTGGTTTGTTGACCAACTTCCAGTGGTTCCAGGCCTCAATTCTCAAC12.4390243902439024No Hit
TTATTACATATATCACCAGAAATCAACCCGAGTGGTTCAGAAACATCCTG12.4390243902439024No Hit
GTTGCCTGGGCCTCATATCCAAAAGGATGGGAACACAGACCACAGCAGCT12.4390243902439024No Hit
GTATCGATAGCAAATAGTAGAATTGCCAAAAATACTTAAGTTCATGTGTG12.4390243902439024No Hit
GGCGGGCTATCCCATCTGAGCATGGCACCACATCAGGAAGTTAATTGTTT12.4390243902439024No Hit
AAAGCAGGAAGACGGAAAACAAACCTGTATGGGGTCATTATAAAAGGAAG12.4390243902439024No Hit
CATTTGGAAGTTTGTTTCATGTATTCGGATTTCCATTTCATCGACGAACG12.4390243902439024No Hit
AGCCTTTACTGATCCACAAGGGCCGAAGTTGTGCGACAATTAGGCAATCC12.4390243902439024No Hit
AAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATCCGAAT12.4390243902439024No Hit
CTTCATACCCTTCATGTACTCGTATTTTCAGTGTTTGGAGGTTGCCAGTT12.4390243902439024No Hit
TACGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAAGTATGCTAGATTCC12.4390243902439024No Hit
AGCCCCTACTTAGCATGTTGAACCTGCCCATCATCCTCCCAGGCCTCAGT12.4390243902439024No Hit
CCCTTCATGTACTCTTATTTTCAGTGTTTGGAGGTTGCCCGTTAGCACTT12.4390243902439024No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG12.4390243902439024No Hit
CACTAGGGCCAGGGTGGGGGATTTGGGCATAATCGAGTGGGTCGGGTCCG12.4390243902439024No Hit
ATTATGGACATTTCATCTACGCACCGGTGCACGCAATAAGGGGAGAATCT12.4390243902439024No Hit
AAATGAGACAGGTCAATTTGGTTTAGGACTATGAGTGATAAAGAACGCCA12.4390243902439024No Hit
ATGTGGACCATATGGCCATAATAAAAAAGTACACATCATGAAGGCAAGAG12.4390243902439024No Hit
CAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCA12.4390243902439024No Hit
ACAAACACAGAGACTGGTGCACCCCAGCTAAACCCGATTGATGTCCCACT12.4390243902439024No Hit
GTCCAGACTCGAAGTCGACCCTGCCATCAAACCGGCCCCTAGACACCATC12.4390243902439024No Hit
ATACTATGTTGATGGATTCGAGCCGAACGGCTGCATTGAGGGCGAGCTTT12.4390243902439024No Hit
TTTGGAAGTTTGTTTCATGTATTCGGATTTCCATTTCAGCGCCGAACGGG12.4390243902439024No Hit
CTTCAGTGCATGGGTGAGGAAGGAGTTGAACCAAGCTGGATTAAGCACAA12.4390243902439024No Hit
CTTAAAAATCGGTCAATACTCACTACCAATCTCTCAGTGCTGGAGTATTC12.4390243902439024No Hit
TCGTCCGTCAATGCACCGGCATCCGGTCCAAGCACGGTAAGGCGGTGGGG12.4390243902439024No Hit
CCACGGCCTCCACCATGCTAGAAATTCCATATGAACTGCTAGGGAAAAAT12.4390243902439024No Hit
GATGGTGGGCTTGTCGGGTGGTATGTGTACGCAGCGCCAGTGAAAGTGGA12.4390243902439024No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)