FastQCFastQC Report
Fri 2 Oct 2020
HTGTLBGXG_n02_S2_R2_B11_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTGTLBGXG_n02_S2_R2_B11_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5305
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTAGGGTGACCTGACTCCATCCCCACTCCAGAACTTGGCCTCAGCACC90.16965127238454286No Hit
AAAGCAGGGTGTGTAGGGTGACCTGACTCCATCCCCACTCCAGAACTTGG80.15080113100848258No Hit
GTTAGGAGGCTACTGTAATGATTTTTAGGCAGGAGATGATGCTGGCTTAG70.13195098963242224No Hit
ATATTAGAGAAGATCTAAATAATTGGAAAAAACATCCTGTGTTCATGGAC70.13195098963242224No Hit
GGGCAGGGGTGCTGGTGATGACCCCACATGACGCCCATCTGGAGGGGGAG60.11310084825636192No Hit
GTCCTAGAGGCAAAAGGGTGGAAGGCAGATGGGGGTGCAGTGTGTTTCAC60.11310084825636192No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)