FastQCFastQC Report
Fri 2 Oct 2020
HTGTLBGXG_n02_S2_R2_B05_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTGTLBGXG_n02_S2_R2_B05_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences43
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATATATGCTGCAATGACGCCACGATCACTTGAATCCCTGAATCTGCACT12.3255813953488373No Hit
GATGTCCATTTAATCTACGAACGTGGTGAATAAAGAAGTGTAGCAGCTGT12.3255813953488373No Hit
TGCGGGATGTGCTTGGGACATTTGACACTGTCCAAATAATACAAATTCTC12.3255813953488373No Hit
AAATAAGACAGGGACATTTGAATTCACACGCTTTTTTTATCGATATGGAT12.3255813953488373No Hit
CACCACTCCGACTAAAAGGTAAAGGGTAATCCACCAACCTATACTTTGTA12.3255813953488373No Hit
CACCAGCACTGTTCGGACCCCGTGGGATTTCCAGTCCTGCAGAGCCTGCT12.3255813953488373No Hit
GTTTTAGATACGTTAGGGTAATGAACGGTACTTGTTGTTGGGCCATTCAG12.3255813953488373No Hit
GTAATACTTCAAGAAGTTGTTGTAAGGCATGCATAAATCTTAATTCTTCG12.3255813953488373No Hit
GCCTGATATCTTCTCTTTTCTGGTTTTCCTATTCAGTCTCCAGCACCCAA12.3255813953488373No Hit
TCTACTTTTCCTAAAAATTCCAGCGCAAAATGCCATAAGCACCACATTCC12.3255813953488373No Hit
TGCCTCAAGAGTTGGTGCATGGGGTTCAGTCGCTGGTTTGCCCTATTGAC12.3255813953488373No Hit
CACGTGTGCTGGAGCTCCACCATCCAGCCGGCAAAAGGGAACAAGATCCA12.3255813953488373No Hit
AAAGCAGACAAACCATTTGAATGGATGTCAATCCGACTCAACTATTACTA12.3255813953488373No Hit
ATTTGAATGGATGTCAATCCGACTCTACTTTTCCTAAAAATTCCAGCGCA12.3255813953488373No Hit
ATACGAAAGCACAAAACTCCAACAGAGCTGTTACAATTGAACTTATTTTT12.3255813953488373No Hit
CATTTTAAAAGGCACGTCTCATTTGAGAAATGATACTGATGTGGTGAACT12.3255813953488373No Hit
GGCAAGTCCTGATGCAATAATTGTGGACAGCCTATGATCACAGGAGGATT12.3255813953488373No Hit
GATGAGCCTTCACCACAGGCAGCAGCCTCTGGCTATCCACTCCACCATAA12.3255813953488373No Hit
CCATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGAGGATTTCT12.3255813953488373No Hit
CTCCAGGAGGAGAAGTGAGAAATGATGATGTTGACCAAAGTTTGATTATC12.3255813953488373No Hit
ACATACTTATGAGGATGTCAAAAATGCAGTTGGGGTCCTCATCGGAGGAC12.3255813953488373No Hit
ATGCACACATTTGGAAGTTTGTTTCATATTTCCATTTCATCGACGAACGG12.3255813953488373No Hit
CTCCAACACCATGTCAAGCTTTCAGGTAGCCTGTTTCCTTTGGCATATCC12.3255813953488373No Hit
TCTTTGTACAGAATCAGGAAGACGGCATCGTTGCCAACCTGTGGAGCAAT12.3255813953488373No Hit
GTATGCTAGAGTCCCGTTTTAGGTTCATTACAAACACAAAATACCCATTA12.3255813953488373No Hit
ATAATGAACCAAGTGGGTATGCACAAACAGACTGTGTTCTAGAGGCTATG12.3255813953488373No Hit
ATTCAATCTAGAGGCCTATTTGGGGCCATTGCCGGTTTCATTGAAGGGGG12.3255813953488373No Hit
NGGCGGCGGGCAGTGGTGGGCCCCCCGGCAGGGGGGGCGCGCCGCCGGCC12.3255813953488373No Hit
AACATAAGCCACACCACCGGTGCTGCTGGAAAGGCAGTGGGGTCCGGGAA12.3255813953488373No Hit
GATTTCTCATTTTGCGCAAAGAACACAAGAGATATGTCCCAGCATTAAGC12.3255813953488373No Hit
GTATGAATACAAAATACTACCATTGCAACCACAAAAAGATTCCATGTTTG12.3255813953488373No Hit
CCTCCAAGATGAACATGATATGTGGCATGGAAATGAGGCCCTGCGTTCTT12.3255813953488373No Hit
ATATAGAAGCAAGACGCAAAGCTCTAAGTGACTGTCAACTAGACCGATTC12.3255813953488373No Hit
GAAACAAACCTGTATGGGTTCATTATAAAAGGAAGGTCTCATTTGAGAAA12.3255813953488373No Hit
GCCCAGGGATCATTAATCAGGCACTCCTCGATTGCTTCATATAGCCCCCC12.3255813953488373No Hit
AACATCACTAATTCACTTTTAACAGACATTAGTAAGACATACCGAGCGGA12.3255813953488373No Hit
GCATCCAGAGAGACACGTAGAAGGACAAGTGCCTCGGGACCGCCGGCGCG12.3255813953488373No Hit
AAGCAATTATAGTCTTTGACGTTTAAGCTGACAGTATAAAGCTTCCGTCT12.3255813953488373No Hit
GTCATTTTCAGAGGCTTGCAGCTTCTGGTTTTCTTTCTCCATTCTGAGAA12.3255813953488373No Hit
ATATGCGTCTCCACAACTTGAGGGGTTTTCGGCTGAATCTAGAAAATTGC12.3255813953488373No Hit
TAACTTATTCGTCATCAATGATGTGGGAGATCAATGGCCCTGAGTCAGTG12.3255813953488373No Hit
GAATAGAACGATTCCTCTTGGGAATCCAGGAATTTGTAGTTGCAACGGCA12.3255813953488373No Hit
CAGACGGGCCACCACATGGTGCTGTGTGCGCGTCCCGCCCGTCACCTGGT12.3255813953488373No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)