FastQCFastQC Report
Fri 2 Oct 2020
HTGTLBGXG_n02_S2_R2_B05-FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTGTLBGXG_n02_S2_R2_B05-FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1650059
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATATACATATTATTAGGGCAGAGTCAACAGTTTTAAGTGAGCGCTCT25410.15399449353023134No Hit
ATATTAGAGAAGATCTAAATAATTGGAAAAAACATCCTGTGTTCATGGAC22450.13605574103713866No Hit
TCCCTGGACAGGCCGGCTGGGACATCACAGCAGGTGCTACAGCCTCCCGA21820.1322376957430007No Hit
CCCCAATAGTTGACGTTTCTATAACTTTAATTCCTCGGGCTTTTGGCCTT21540.13054078672338382No Hit
CCATATGGATGAAGCAGACATCCTGGGGGACAGGATTGCCATCATTTCCC20390.1235713389642431No Hit
GTCTTAAGTACTATTGCTTTATAGTAAGTTTTGAAATCAGGAAGTGGGAA19650.11908665084096993No Hit
GTATTAACTCTGAATAATAACGAGTCTACGTGGATGCCCCATCCTACCAA19080.11563222890817844No Hit
GTGTAGGGTGACCTGACTCCATCCCCACTCCAGAACTTGGCCTCAGCACC18520.11223841086894469No Hit
GTCTAGGGGAATCCCAAAATTTCTAGCCTGGGCAATCAGAAGGATTCGAG17790.10781432663922927No Hit
GTATAAAGTAGGTAAACCTCTAGCTTTAATCTTTTGCCATCTCTGATCTC17450.10575379425826592No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA9550.080.545581
GCGAAAG14800.051.4458662
GTTGACG7600.050.5108539
CCAATAG8650.049.4185453
TATATAC7800.049.230623
TAGTTGA8300.047.1249857
GAGCGAA2950.046.7380751
AGGGTAT1903.6379788E-1241.9335376
CCCAATA10000.041.3330762
GTATTGG907.4439085E-540.3150061
CTAGCAA2400.039.240414
CGAAAGC19250.039.1432343
CCCCAAT9900.038.8490071
AATGCGA750.001490659138.6296846
TATACAT10350.038.493225
ATAGTTG10600.036.8986366
TTATATA10500.036.602392
TACATAT11100.034.5850567
CACCCGT2404.3655746E-1133.198397
GTGTAGG7250.032.029581
Produced by FastQC (version 0.11.5)