FastQCFastQC Report
Fri 2 Oct 2020
HTGTLBGXG_n02_S2_R2_B04_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTGTLBGXG_n02_S2_R2_B04_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences113
Sequences flagged as poor quality0
Sequence length151
%GC49

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTTAAGAGAGTGGCCTCTTACTGTTTGCTTTTTGAGTCCTGCACATGGA10.8849557522123894No Hit
GCATCCTACTCGGTTCTGGTGGGTCAGCAGCAAAGGGGAGCAGTGTTAGT10.8849557522123894No Hit
GGGTGTGGGTAGGGAGGTTGGGTTCCGGAGGGGGGTGGGGGCGAGGGTGC10.8849557522123894No Hit
ACCCAGGTGCCCCCCAAGTGGATGTTTTGGACGGACTGTCCTGGTTTGTG10.8849557522123894No Hit
CCCCTTGGCTGTTTTGGAGTTAGTTGCATGGGTCTATCCCGCCCAGTGCG10.8849557522123894No Hit
CGGGTGGGTGGTGGGGGCGGGAGGGTGGGGCGGGTGGGACGGGGGTGGGC10.8849557522123894No Hit
CCTCTATATGGAAGCATTGTATTTACTTTTTCTTATATATCAGTTTATCT10.8849557522123894No Hit
ATGGCGGGGTTATTTGCAAGGATGGGGGTTGCGGGGTGATTTCCAGGTCG10.8849557522123894No Hit
ATGTGATGTTCCAGCAGAGATCTGCTGAATAGCTATGCCATCTAAGCCAC10.8849557522123894No Hit
CCAGTTGCACATGGCTGACTGGGAGACAGAAGAGGTTGGGGAACCTAGGT10.8849557522123894No Hit
GTATGAGAGCTGCCAAGCCTCCATTTTAATAGCAGTTCAGGGAGTCTGTG10.8849557522123894No Hit
TCCCCAGCCTTGAAAACTTTAGAGCCTATGTAGATGGATTCGAGCCGAAC10.8849557522123894No Hit
AATCAACCGGCAGCAACTGCATTGGCCAACACCATAGAAGTCTTTAGATC10.8849557522123894No Hit
GTTCCCTAGGGCTTCCTCAACTGTTTACTGACTCATCCTCACCCTCTGTC10.8849557522123894No Hit
GGCATAAATCCCAATTACCTCATGGGTTGGATGCAGGTGCTAGCAGAGCT10.8849557522123894No Hit
ATGCGCAAGCTTGCCGACCAAAGTCTCCCACCGAACTTCCCCAGCCTTGA10.8849557522123894No Hit
GAATAGGAATGGCCCAACAACAAGTACAGTTCATTACCCTAAGGGAGATA10.8849557522123894No Hit
CATGAGGCTGACCACTTGGCTGTTTTGGAGTAATTTGAATGGGTCTATCC10.8849557522123894No Hit
GGGACCCGCCTTGTTGTAGTACTTATCCCTTCCTTGCTGGAGGAGAACAC10.8849557522123894No Hit
TGGTGGAACAGATCTTCATGATCTCAGAGAACTCTTCTTTCTTGATCCGT10.8849557522123894No Hit
GTACACAGTAAACAGAAAACACCAGAGAATGGTGCACACCAGCTACACCC10.8849557522123894No Hit
CCCATACACACAAGCAGGCAGGCAGGATTTATGTGCAACTGATCCCCTCA10.8849557522123894No Hit
GTCGGCACTTGATGAGACGGCTTGTGAGTATGGACTTGTGGACACGGGGC10.8849557522123894No Hit
GGGGGCGGGGGTGGGTCGAGTAGGGGGGCGGCGCGGCCGGGGGGGCGCGG10.8849557522123894No Hit
TTCTTGATCCGTCCAGACTCGAAGTCGACCCTGGCCTCAATCCGGGCCGA10.8849557522123894No Hit
GAATACCACTATATGATGCAATCAAATGCATGAAGACATTCTTTGGCTGG10.8849557522123894No Hit
AAAGAGAATTGGTCCGTAAAACAAGGTTTCTCCCAGTAGCCGGCGGAACA10.8849557522123894No Hit
CTTCAACTCAGTAATTGAGTTATCTGTTTTTGGTTATTTGGTCACCACGG10.8849557522123894No Hit
CTTACCCGGTGAGTTCTGCTGGTCTAGGAATACTCGTACTAATATTCGAA10.8849557522123894No Hit
CTATATGATGCAATCAAATGCATGAGGACATTCTTTGGCTGGAAAGAGCC10.8849557522123894No Hit
CCTCCACACTCCAGTGGAGTCCAGCCCCTGGTCTCTTCAGCATTGGCTAA10.8849557522123894No Hit
GCCCAAAACTGTACCACAGCAAGAAAGCTGGGTAACTTCCCAAGAGGTCA10.8849557522123894No Hit
TTGCTCATTGGAACATTTCAAACATTGATGCTAAGCCTCTGCATTCCACT10.8849557522123894No Hit
GGCGGGGGGGGGGGGGGGGGGGGGGGGGGGTGGGGGGGGGGGAGGGAGGG10.8849557522123894No Hit
GCACCAACTTTCTTCCACCATTTTCTTTAGTTTCAGTGCGTCTATCATAA10.8849557522123894No Hit
GTACTGAAGCAGTCAGGTGCTAGAAAGACAGCTCTGCTTTGAGGAAGGGT10.8849557522123894No Hit
CAGTACACTACTTCTCCCGCCCACCAAGGCCACCCTCTGGGGACGCACGT10.8849557522123894No Hit
GCTCTACAAGCTTGAGTCAGACAAGCGCCTGGTGGAGGAGAAGGTGAGCC10.8849557522123894No Hit
CCCTTGGCCTCGATATCGAAACAGCCACTCTTGTTGGGAAACAAATCGTG10.8849557522123894No Hit
GGGCAGAGGAGTTCCAGAAAGTTCTTTATAGGCTACACCCTTTGTGCTCC10.8849557522123894No Hit
GTTTGGTCCTCCATCTGATACTAATAGCCCTACCTTTGATTGGGTTTGAT10.8849557522123894No Hit
TGCTTGCGATATGTTCAATCGGGGCAACATCTTCTCCTATTTCATCAAGT10.8849557522123894No Hit
TCCAAACACACACCAGTGCCAGAAAAGCAACAGACAAGCATTTGGTTCTC10.8849557522123894No Hit
CTATAAGACAAGAAATGGCCAGTAGGAGTCTATGGGATTCCTTTCGTCAG10.8849557522123894No Hit
CCCAGGCACCCTGAGAAGGTTAATTTTTTAAAAAAGTGTTTTGGTTTTGG10.8849557522123894No Hit
GTTATTATCTGTGTTCTAGAGATAAGGATGTGTAGTTCAGAGAGTCATTC10.8849557522123894No Hit
AGTCTCATACTCTGGAGATTGTTCAAGAATGTGTTGGTATTTGGCCAGCT10.8849557522123894No Hit
GTGGGGGCCCGCTGGTGGGGGGGGGGGGGTGGGGGGTGGGGGGGCGGGGC10.8849557522123894No Hit
CCAGTATTTAGAAGCCAACCGTGATTATTAATGATGTCCAACCCACCTGA10.8849557522123894No Hit
CTGTAAACAATGCTGTGGTAATGCCAGCCCATGGTCCAGCCAAAAGCATG10.8849557522123894No Hit
GCTTTTAGCTTCAATGAGGTCTTTGAAATGTATAATGAAGGTGTAATTTT10.8849557522123894No Hit
TCTGTAGTCTTTGATGAACAATTGAAGAGCCATCTGGGCCGTTGCAGGTC10.8849557522123894No Hit
ACCACAGTCTTCCTCATCTGTGCCCTTTTCTTGGGCAGAAGCAGTGGTTC10.8849557522123894No Hit
GACTTTGCCTCCCCAGGGGCGGAGGTGTGTCCCGGAGTCGGGGGCAGTTG10.8849557522123894No Hit
CTGCTATTAAAATGGAGGCTTGGCATCTCTCATACCATATTTTAATATGC10.8849557522123894No Hit
AGCTTGACCAGACAAATCAGATCTGTAGCTGATTCTGATGTTGAAGTACA10.8849557522123894No Hit
GGCCACATACATGGGCCAGGCTTACGTGCCTGGACTTTTCTGGAACCCAC10.8849557522123894No Hit
GTGTTTTTTATCATTAAATAAGCTGAAACGAGAAAGCTCTTATCTCTTGT10.8849557522123894No Hit
TCAGATATGTTGTCAAAATTTTTCTTGCCTGTTACCTAGGAGATAAAATC10.8849557522123894No Hit
GTCCCACAAAGGAGCTGTGGCACACTCAGAACGGGAGAAGAGCTTCAGTC10.8849557522123894No Hit
CTGGCAGGCCTGTGGCCCTTTCCGAATGATGGAGTCGATCAGAGCCCGGG10.8849557522123894No Hit
GAAAGAAATCTCTGTGTCTGGTGAAGGAGGCAGCATCTTTCTCACCCCAT10.8849557522123894No Hit
ATATACTAATTTCTGTGTGTGTGTGTGTGTGTGTGAGGACCTAAGGAGAA10.8849557522123894No Hit
ACATGGTGGAATAGGAATGGCCCAACAACAAGTACAGTTCATTACCCTAA10.8849557522123894No Hit
GGCCAACACCATAGAAGTCTTTAGATCGAATGGCCTAACAGCTAATGAGT10.8849557522123894No Hit
GTTAAATTCATTATTTTTGCCGTCTGAGTTCTTCAATGGTGGAACAGATC10.8849557522123894No Hit
GGATGCTTGGAGCCCCTCCCCCCAGTATATTGTCTTGGTGGATTTCTGTT10.8849557522123894No Hit
GAAGAGGCCTTATTTTCTCAATTTTCTTCTTTGTTGATTCATTGAAGTAC10.8849557522123894No Hit
GGGTAGGAGCGGTGGCCACTTCCCTCAGAAACTGTCGATGCCTCTCAGAA10.8849557522123894No Hit
CCTCAAGAGCATGTCTCCTATTTCAAGAACACAGTATTTTTCCCATTTGT10.8849557522123894No Hit
GCCTGCAGCTGAGACGTATATCACATCTCTTAATTTTTCTCCAACGCTGC10.8849557522123894No Hit
GTCGTGAATTAATGAAAGGGGTCCCAGAGCAGGAAAAGAGTGAAGTGCTC10.8849557522123894No Hit
CAGTTGGGCCGTTGCGATGCTTCCGCATCTGGCTGCGGAGGTCAGCGACC10.8849557522123894No Hit
ATTAAGTATTGAAGACCCGAGTCACGAGGGGGAGGGAATACCACTATATG10.8849557522123894No Hit
GCCCAGGCTCTGACAAGGTGTTCTCCTCCAGCAAGGAAGGGATAAGTACT10.8849557522123894No Hit
GCTCAAAGTCATCCATGGCTGCACACGATCCATAGACCAAGGCCCTATTA10.8849557522123894No Hit
TCCTTACTCCTCCAATCTGTGTGCTGTGGCACATTTCCAAGAGAGATGCT10.8849557522123894No Hit
CCGGTGGCGTGGCGCGTGGGGGGGAGTGGGGGCGGTGGCCGTTATGGGCG10.8849557522123894No Hit
GGCCCTCTTTTCAAACCGTATTTAAAGCGACGATAAATACATTTGAAAAA10.8849557522123894No Hit
GGCCAGGCTTACGTGCCTGGACTTTTCTGGAACCCACATTAAAAGTGAGT10.8849557522123894No Hit
GTGCGGGGGGGGGGGGGGGTGGGGGGGGGGGGGGGGGGGGGGGGGCCGGG10.8849557522123894No Hit
CCCCCCACCCAGGGTTGGGGTGCAAGACGGGAGGACGCTGTAGCCATGAT10.8849557522123894No Hit
GAAGTGAGGAGTAAATCGCTTCCCGATCTTTTTTGTTGGGTCTATACAAC10.8849557522123894No Hit
GTTCATTACCCTAAGGTATATAAAACTTATTTCGAAAAGGTCGAAAGGTT10.8849557522123894No Hit
GTTGTAATGTCGCAGTCCCGCACTCGCGTGATACTCACTAAGACCTCTGT10.8849557522123894No Hit
GTCTTGGTGTATTTCTTTTGTCCAAGATTCAGTATCGAGACTCCCAAGCC10.8849557522123894No Hit
GTCAATGAGGAAAATTGCATCCTACTCTGTTCTGGTGGGGCAGCAGCAAA10.8849557522123894No Hit
GTGTTCTAGAGGCTATGGCTTTCCTTGAAGAATCCCACCCAGGAATATTT10.8849557522123894No Hit
GCTCTTCATGATCTCAGAGCACTCTGCTTGCTTGCTACTTCCAGACTCGA10.8849557522123894No Hit
GAACAATGAGAAGCAATTTTCTAGATTCAGCCGAAACCCCCTCAAGTTGT10.8849557522123894No Hit
TAATTATACGACACTTCAGAAAATTCACAAAGTGTTAACGAATCACGCAA10.8849557522123894No Hit
GGATCAATCTCCTGGTTGCCTTTCTGAGAATAGCTGTTGCTCTTCTCCCA10.8849557522123894No Hit
GCAAAACCCAGGGATCATTAATCAGGCACTCATCGATTGCTTCATATAGA10.8849557522123894No Hit
TAAGTATGTCCTGGAAGAGAAGGTAATGGTGAAATTTCTCCAACTATTGC10.8849557522123894No Hit
CTGGGATGCTCTTCTAGGTATTTATTTCTTCTCTCATCAAAAGCAGAAAG10.8849557522123894No Hit
GATTGGTGGCCCTATAAAATTATGATGGGTAATGTCAGTCAGTCCTCAGC10.8849557522123894No Hit
CGCGGGTAGTTTGCTTGCCCCTTGGGGCCGTGTCTTCAGGTGCAGATTAG10.8849557522123894No Hit
GTTCCTGTAATCTCAAATTTTTCTTCAATTGTCTCTTCGCCTCTTTCGGA10.8849557522123894No Hit
GAAATTAAGTATTGAAGACCCGAGTCACGAGGGGGAGGGAATACCACTAT10.8849557522123894No Hit
CTGTTACTCCCATTGGCCATTTAACAGGTGGTACATGGCGCCTAGGTGGC10.8849557522123894No Hit
GAGCGGGTGAGGGTGAGCAGGGGAGAGGGGGTGAGGGGGAGTGCGAGGGG10.8849557522123894No Hit
AAGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACAATGCTTCAATC10.8849557522123894No Hit
AGCTCATTGTTTAGTTTGTGTCCTCCCACTTAATCTTGTTTTCAGTTTGG10.8849557522123894No Hit
AGAGACAACATGACCAAGAAGATGGTCACGCAAAGAACAATAGGGAAGAA10.8849557522123894No Hit
GAGTCAGAGCTAGTCAACCCTTATCAATGGAGAATCAGGAACTGGGATAT10.8849557522123894No Hit
ATGTATAGGTTATCATGCGAACAATTCAACAGACACTGTAGACACAGTAC10.8849557522123894No Hit
TATATGCACTGCTAGGGACACAGTTAGCGAAGAGAGTGCATCACTTCTGG10.8849557522123894No Hit
ATTATTGCCTCAGCAATTGACTGCTCGTCTCTCCCGCTTACTATCAACTG10.8849557522123894No Hit
CTTGTCTTCTTTGCCCAAAATGAGAAATCCTCTCGGGACAGCAGACTCCA10.8849557522123894No Hit
GTGAGGAGGGACCTGCAAGGGGCTGGGTGGCCTGCCGGGCTGACCCCAGC10.8849557522123894No Hit
ACCCTAGATAAGTCACTCTTCCTCCTTGAAGTCTCTATAAAATCTGTAAA10.8849557522123894No Hit
GAGATCGGGGAGACCAGCGGGGTTTTCCAGAGCGGTCGCCGCCGTGAGGA10.8849557522123894No Hit
CTATTAGAAGAGGCCTTATTTTCTCAATTTTCTTCTTTGTTGATTCATTG10.8849557522123894No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)