FastQCFastQC Report
Fri 2 Oct 2020
HTGTLBGXG_n02_S2_R2_B03_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTGTLBGXG_n02_S2_R2_B03_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences44
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATACAAGACGTTACGGTTGAGAATATTGATGTGGATAAACAAAAAGACAA12.272727272727273No Hit
GTGATGGACCGTTCATGCCGGCTCCTGACATCACCTAGACAAGGTCCTGC12.272727272727273No Hit
GACCATGGTTACCAAATTCACATTTGAAGCAAACTGGACCAATCTAGTGG12.272727272727273No Hit
ATGTTGACCAAAGTTTGATTATCGCTGCTAGAAACATAGTAAGAAGAGCA12.272727272727273No Hit
CCTTATTTATAATCGAGGAAATGGAAGCCCCGATAGTTAAGATAGCAGCT12.272727272727273No Hit
TACCGGGGTTCTAATCTAGCCGTCCTGATTTGTAATTTTTGATTATGGCC12.272727272727273No Hit
CTGCAGCTCTTCAGCGCGTAACCAATGTTCTCCCTCACACAACCCGCGAA12.272727272727273No Hit
ACTCTAGCGGGGCTGTGTGGCACAGTGGTAGTGGCCGTGGGCTCTGACGG12.272727272727273No Hit
GAACCCCGCACTCAGAATGAAGTGGATGATGGCAATGAGATACCCAATTA12.272727272727273No Hit
GGATTAGAGTAGCCTAATGCCACAGAGAGAAAGGCTCTCGGAACTTTAGC12.272727272727273No Hit
GGTGTCTGGTGAGGGCATTCAGGAGTTCCTGGGTGCCCAAGTCCTCAGGA12.272727272727273No Hit
AGTAGAAACAATGTCGTTTTTAAACAATTCGAAACTAATTGATGGCCATC12.272727272727273No Hit
TATACATATTATTAGGGCAGAGTCAACAGTTTTAAGTGAGCGCTATGGCT12.272727272727273No Hit
CAACTTTCAAAAATATGAAAAAAGAAAAATAAACAGAAAAAAATAAAATA12.272727272727273No Hit
CATAATAAGAAGAGCGGCAGAGTCAGCCGCCCCATAAGCCTCTCTATTGG12.272727272727273No Hit
GAACAGGAAGTAGTAAGATTCGAGTAATTTTTACCAATCAGATCATAAAT12.272727272727273No Hit
CTCTCTGGGCTGTGGGCAATGAGGCCGAGACCGTTCTGGTTTCTAATAAG12.272727272727273No Hit
GACTGTAGTCTGTGCAAAGACCGAGGTACTGAAGGATTTGAAATAGCCCT12.272727272727273No Hit
TCTATAGATAGGACCCTGGGATTCAGAAGCAGATTTGTTAGCAGAAGACT12.272727272727273No Hit
GTCAGTAAGTATGCTAGAGTCCCGTTTTAGATTGCGTACCACAACGACGT12.272727272727273No Hit
ACTCTCATACACCTGTGCATTGTATACGAAGAAATAAGACACTTCATTAC12.272727272727273No Hit
GCACTGGAGCTAGGATGAGTCCCAATAGTTCTCATTGCATGTACCATCTG12.272727272727273No Hit
CCCTGGACGAAAGAGAGAATTTTAGCTGTAAAAATCAGTGGTGCTCTCAG12.272727272727273No Hit
CATTCAGTCTGGTTACCTACTGCTGTTTCACAAATCACTCAGAAATGTAG12.272727272727273No Hit
ACAAATATCCTTAGCTCGGCAAAACGCAGAGAAAAATAAAATAAATGGAG12.272727272727273No Hit
AGCCACAGCAGCACCTGATACAGCTCTGAATACCTTTTCAGTTTTTCCCC12.272727272727273No Hit
GTATGCTAGAGTCACGATTTAGTTTCATAACAAACAAAACCTACCCGTGC12.272727272727273No Hit
ATAAAAAAGATTGCATGTTAAAAAAGAACACAAACGCTTTCTTCAATAAT12.272727272727273No Hit
ACATAGCACTGTGAAGAATAAAGGGCATTTACAAAGGCTGTCTGAGCAAA12.272727272727273No Hit
CCTTTTTTCCATCACTGATATAGTAGTATGAACTGTCTTGCATTTGATCC12.272727272727273No Hit
GGGTTAGGATGGGGAGCCCCAGACTCTGGGTGTCAGCCTGAGGGCGGGAG12.272727272727273No Hit
GGATTAAGAGGGATACCACCCAGAACTCTTCTTTCGAGAGCTGTGCACTA12.272727272727273No Hit
CCCCAAACACAGTCTGCATAGAGCCCAAGACAGAAATAGCCACAAGAACG12.272727272727273No Hit
GATATGCATCTTGGAGACACTCATGAGCAAACCGCACATCAGAGGAATTA12.272727272727273No Hit
TAATAATATGTCTGGGCTACTCCTTTTCCACTTCTAGAAAAGCAGATAAA12.272727272727273No Hit
CAATCTAATTACCCTGAGATAAACAAGGGAGTTGCGCTAGACAGTTAAGA12.272727272727273No Hit
GCCCCAGACTATCTCGTCAACATGGTGCCAGCTCTTTGGGGCCCTGAAAT12.272727272727273No Hit
CTTCCATGAGATTGATGACCGACTCTCAGACCTGGGCATCTCTAGCTATG12.272727272727273No Hit
GAGTAGCACAGAAATATTATTATTTAGAAGAAACTTAATAGCAAAGAAAA12.272727272727273No Hit
AATATATACTGCACACTAGGATATTTAAGATACATGACGTGAGATGACTT12.272727272727273No Hit
TCAGAAGCCTCTTCAGGAGTGAGACCTATGCTTGAGAACACAACTGATGA12.272727272727273No Hit
ACATGACCCCAAGCACTGAGATGTCGCTGAGAGGGATAAGAGTAAGAAAA12.272727272727273No Hit
GTCATCAATCTCATGGAAGAGTTCCACGAATGCCCATTCTTTTGCAGCTT12.272727272727273No Hit
GAGAGTCAGCAGATCGAGAGAATGTAGGGCATGAAAGAGTAAGAGGAGTC12.272727272727273No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)