FastQCFastQC Report
Fri 2 Oct 2020
HTGTLBGXG_n02_S2_R2_A12_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTGTLBGXG_n02_S2_R2_A12_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCACTGACATTAAATACGATGCCCAAAGATGCAGAGAGAGGCAAGTTAA14.0No Hit
TAACAACCCACTTTCAAAGAAAAAGGAGAGTAAGAGACAACATGACCAAG14.0No Hit
ATGCAACCAAAGAGGGCTAGCCCCCTTGCAGTTGGGTAAATGTAACCTTG14.0No Hit
GGGGAATTCTTGAGGATGCACAGATGTACCAGAAGTGCTGCAATCTATTC14.0No Hit
CTACCACTCTCTCCGTGCTGGAGTATTCATCTACTCCCATTTTGCTGACT14.0No Hit
CCCCAGGAGGGGTGCGGCTTGCTTGCGGTGCGGGTGTGGAGGGCAGGGAG14.0No Hit
CATAGGGACAACCTGGAACCTGGAACCTTCGATCTTGGGGGGCTATATGA14.0No Hit
ACAGAGTAGGATGCAATTTTCCTCATTGACTGTGAATGTGAGAGGATCAG14.0No Hit
GTTTTATATACCTTAGGGTAATGAACTGTACTTGTTATTGGGCCATTCCT14.0No Hit
AGCCCGTACTTAGCATGTTGAACCTGACCCTAATACGAACAGGACTCAGT14.0No Hit
CTTTATAATAGGTACACTCCTGCTTGGATTAAATAATGTTTTGGTTCAGT14.0No Hit
GTTTGGAGGTTGCCCGTTAGCACTTCTTCTTCTTTCTTGAATGATGATCC14.0No Hit
CGTCTCACGGGCAGCGCCAGCTAAGTGCTGGGGGGCAGGTCTGGCAGGAG14.0No Hit
GCTTAAAATGGGCGCTAATGGATGATGATTATCGGGGCAGCCTTTGTAAT14.0No Hit
AACATAATGAACGCACTGTGCATTTATACCCCCATCCTCTATGGAACCAG14.0No Hit
GATGAAGACTAATTCACAAGACACAGAGATTTATTTCACAATCACTGGGG14.0No Hit
TCCGTTGCCAGGATCCAACTGCGGGACAAGCCGGCGGCATATGCCAGGGC14.0No Hit
GGTGCGGAAGGATACTGGTGGCGTGTGGGGGGGAAGGCTGGAGTGCTATC14.0No Hit
CGTCCACATATAGTACCTAGAGAAAGCCAACAAAATAAAAGCTGAGAAGC14.0No Hit
GGGTGCTCACTGGTCGCGATAGCCCCATTCAAATTACACCAACACCGCCA14.0No Hit
TTTGGGTCCCGCTCGGGAGGGGGCGCTTCTGACCTTCGTGTCCGTGCAGC14.0No Hit
CTCATATGGTCCATAACGTCTTATTCGCAATTGATTGAATATGGGCAATC14.0No Hit
CCTGAAGTCTGCTTAAAATGGGAGCTAATGGATGATGATTATCGGGGAAG14.0No Hit
CTGCACTCCCATTCGCTTCTGTTAGGACTGCAATTTTTCAAGAAGGTCAG14.0No Hit
GATACAAATATAGGTGAAATAGGGGAGACACACAAATACAGCCGAAATGA14.0No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)