FastQCFastQC Report
Fri 2 Oct 2020
HTGTLBGXG_n02_S2_R2_A06-FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTGTLBGXG_n02_S2_R2_A06-FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1208072
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAGACCTCTTAACAATATTGTTCTTCCAATCCATGAGCATGGATTGT17160.14204451390314485No Hit
GTACTGAAGAACACAGTAAGTCAGGATGGGACAAAGGCAAGGCTGGAAGG14010.11596990907826686No Hit
GTCCTAGCGAATGGCCCTGGCACTCGCAGGCTTGTGGAAACGGCTAAGCT12160.1006562522763544No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA6650.063.2742351
CGAAAGC7500.056.9948273
GAGCGAA1051.1023076E-955.274041
TAATGGT4200.051.7336124
TACGCAT604.969085E-448.286715
GCGAAAG9300.046.003582
ACATCGA2850.045.743418
GACCGCC803.757387E-545.2669148
ATTGTAC1002.7473507E-643.4706343
TATAAGC2350.043.162333
AACCGGT3550.042.8441776
AGACCGC855.3739728E-542.6041567
GCGATAT700.001053350441.4555361
CATCGAG3150.039.0892379
GTATACG1001.3863038E-436.273591
ATCGTGA1301.6528607E-533.4680522
TATACGT1102.4508862E-432.9213944
CGAATAG3150.032.2431951
ACCGGTG4800.031.686847
ACCGCCA1153.1804363E-431.4913319
Produced by FastQC (version 0.11.5)