FastQCFastQC Report
Fri 2 Oct 2020
HTGTLBGXG_n02_S2_R2_A05_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTGTLBGXG_n02_S2_R2_A05_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTCTGAAGAAAAATAATGGGGGAAATGCATACCTCTACTTTTACTCCCC13.0303030303030303No Hit
GTACGGTTATCACCATCAAAATGAGCAGGGGTCAGGATATGCAGCCGACC13.0303030303030303No Hit
GTATTACATGAGGGCATATTTTCACAAATTTCATCTTATTTGATTGATTG13.0303030303030303No Hit
ATATACGGAATCTTCACATTCCTTAAGGCTGCTTAAAATGGGAGCTAATG13.0303030303030303No Hit
CACATATAGGTGCCATAGGGTAGACACACAAATTCAGACGAGAAGATCAT13.0303030303030303No Hit
TTGAATGTTATTATCCCTTAGTAAGTTAACACAGCCACAGCCCAAGTGTC13.0303030303030303No Hit
GTTTGGGGGTTCCGGGGGAGTTAGGGGAGGTGGTGATGGGGGGGGAGGGA13.0303030303030303No Hit
CTAACAAGATTGCTGAAATATGCACACATTTTGAAGTTTTTTTCATCTAT13.0303030303030303No Hit
GAGCAGGAGCAGGGGCAGGAGCAGGAACAGGTGCATGAAAACCAGCAAGA13.0303030303030303No Hit
TGATAAATCTCTAGCTATTACTGCAAAGCCCGAGAGTTGGGCTTAAATTT13.0303030303030303No Hit
CCATGGCCTCCACCTTGCTAGAGTTTCCAACCGGTCTCCTATATGAACTG13.0303030303030303No Hit
GTAGAAACAAGGTACTTTTTAGGACAGTATGGATAGCAAATAGTAGCATT13.0303030303030303No Hit
AAATGATACTGATGTGGTGAACTTTGTAAGTATGGAGTTATCACTCACTG13.0303030303030303No Hit
GATCATTGATGCTTAATGCTGGGCCATATCTCTTGTCTTCTTTGCCCAAA13.0303030303030303No Hit
TCCCAGGACCCTAGGATCATGACCTGGGAGGAACGCACAGCTTTAACCCA13.0303030303030303No Hit
CTAGAGTTGTATCTGTCGTCTGTTCGGACCAATGGCAGATCCATAGGTGC13.0303030303030303No Hit
AGTAGAAACAAGGCATTTTTTCATGAAGGACAAGTTAAATAAATTATTTT13.0303030303030303No Hit
CCCTATAAATTATAACTTTGTAACTCCATCAGATACTTATATCCATAAAT13.0303030303030303No Hit
CTACAGACGCGCTAAGTGAACTTCACAAGCGCAGCCGTACTTGGTGGATC13.0303030303030303No Hit
GCAGAAGAGGGAGAAGCAGGCTTCCCACAGAGCAGGGAGCCCAACATGGG13.0303030303030303No Hit
CTTTGGATGGAAAGAGCATAACATAGTCAAACCACATGAGAAAGGCATAA13.0303030303030303No Hit
AATGCAGTGTGATCCGATTTGATCCTTACCTTTCCTCAGCTGGATTTCTG13.0303030303030303No Hit
TGATGGTGGCGTACATGCTAGAAAGAGAATTGGTCCGTAAAACAAGGTTT13.0303030303030303No Hit
GTGTTACATATACTGTTCCCCACTGTCCAGGCCATGATTCGCTCTCTTAC13.0303030303030303No Hit
CCCTACCACTCTCACCGTTCTGGAGGATACATATGCACCAAAATAGCTGG13.0303030303030303No Hit
AGTATATTGTCTTGGTGTATTTCTTTTGTCCAAGATTCAGTATCGAGACT13.0303030303030303No Hit
ACTTGGAGGTCATGTAGAATCTGTCCACACCTGAATGGATTCCCTGATGG13.0303030303030303No Hit
CTGTCCCTCTGCACGCTCCTTACTCTGGCGCACAGGAACTCCCCCAGTCC13.0303030303030303No Hit
ATTATATATATAAAATATTAGAATTCCAACAACTACTTAAGTCAATCTGT13.0303030303030303No Hit
AAAAAGCCCCTGTTACCCTCGGATTCCTGGTCCCCTTCAACGTAATGTAG13.0303030303030303No Hit
CAACGTACTTATTTGCACAGTAAGTATAGCAAAAATTAGCATACCAACAA13.0303030303030303No Hit
TCCGTGGGTAAGGCGTCTGGGCAAGAAGTGCGCGTGCTCTGGGGCTTTCC13.0303030303030303No Hit
GGTATGGAGCCCAGGCCCAGGAAATCCGTCTCTAGAAGGAGGCCCTCGAA13.0303030303030303No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)