FastQCFastQC Report
Fri 2 Oct 2020
HTGTLBGXG_n02_S2_R2_A04_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTGTLBGXG_n02_S2_R2_A04_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences79
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAATCGGAAACATGGCCAATCGGAGAGTCACCCAGGGGAGTGGAGGAAG11.2658227848101267No Hit
TTACAGAACTGGACTTATAAAACCGAAGAACCCACAGGAACTAAAGGACC11.2658227848101267No Hit
GGACAGCAGACTCCACCCCAGATGTGCCTTCATCTGGATCTTCAGTCAAT11.2658227848101267No Hit
GTTGAGCAGTCCTCCGGGCGCTTCGGGGCGATCAGGGGTCAGGACAAGGC11.2658227848101267No Hit
GATGTCAGTATTCTTGCCCCCACTTCATTTGGGAAAACAACTTCCATAAT11.2658227848101267No Hit
GAGAAAGGAGGAAGCAGGCTACTCGCTGAGCAGGGAGCCTGATGCGGGGC11.2658227848101267No Hit
TGAGAGAGCTGAGCGTTTGTGCCCGTGAAGCGAGGCGGAGGGGTGGGGGG11.2658227848101267No Hit
AGCAAATGCCAAGTATTTTCCAGAGGAAATTCCCTGACTTACAAAATGTC11.2658227848101267No Hit
CACTGACCCGAGACTGGAGCCACACAAATGGGAAAAATACTGTGTTCTTG11.2658227848101267No Hit
AATTACCTCATGGCTTGGAAGCAGGTGCTAGCAGAGCTACAGGACATTGA11.2658227848101267No Hit
ATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACT11.2658227848101267No Hit
GTGCTGGGAAATCAAGGGAGAGGATCAAGCATAGGATGGAACTTTCCTGA11.2658227848101267No Hit
CCCTAAGGTATATAAAACTTATTTCGAAAAGGTCGAAAGGTTGAAACATG11.2658227848101267No Hit
TGGCTGGACCATGGGCTGGCATTACCACAGCATTGTTTACAGAATCAATC11.2658227848101267No Hit
ATCATATGCAACAACACATACAAAGGCTCCAAAGAGCCTAACAAACTCAA11.2658227848101267No Hit
CCAAGGCACCATTCTGTGCCTGTTGACTATGATGTCTACTTGATTTAAGT11.2658227848101267No Hit
GGGTGGGGGAGGCAGGTGTGGGGCTCAATCCTAGGCCGGTGGGATGATGC11.2658227848101267No Hit
GAATAAGAGAGGCTATCTAATAAGAGCACTGACATTAAATACGATGACCA11.2658227848101267No Hit
NGCTTAATGCTGGGCCATATCTCTTGTCTTCTTTGCCCAAAATGAGAAAT11.2658227848101267No Hit
GGTGTATTTCTTTTGTCCAAGATTCAGTATCGAGACTCCCAAGACCGTAC11.2658227848101267No Hit
AAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCTACTTTTCCTA11.2658227848101267No Hit
GGCTATTAGTATCAGATGGAGGACCAAACTTATACAATATACGGAATCTT11.2658227848101267No Hit
TGGCTATACAGGGTCTTTTGTAGTTAAATAAAATTGTTAGACTTGTTTTA11.2658227848101267No Hit
GCCATAAGCACCACATTCCCTTATACTGGAGATCCTCCATACAGCCATGG11.2658227848101267No Hit
GTCCCCATCCACCGCACGCGGCTGTTCGTGTTGATATTCTTCCCTCTTGG11.2658227848101267No Hit
GCCTATGTAGATGGATTCGAGCCGAACGGCTGCATTGAGGGCAAGCTTTC11.2658227848101267No Hit
CTCCTGTGAGAATACCATCCCCACAATTATTGCCTCAGCAATTGACTGCT11.2658227848101267No Hit
CTACAAGGAAGAACTAGTTCTCGAGAAACCCTTCTCATTTTTACCACTCA11.2658227848101267No Hit
GCGATAAAAAAAGCTTGTGAATTCAAATGTCCCTGTCTTATTTATATAGG11.2658227848101267No Hit
ACAATATCAGGTGCAAGATCCCAATGATATTTGCTGCAATGACGAGAGGA11.2658227848101267No Hit
ATCTGTGGTCAGTACTGCCCAGGATTTATGGAAAATGGGCTGTCAGACAA11.2658227848101267No Hit
GATGGGCTCCAATCATCCGACGATTTTGCTCTCATAGTGAATGCACCAAA11.2658227848101267No Hit
TCTGTAGTCTTTGCTGAACAAGTGCAGCGCCCTCGGGGCCGTTGCCGGTC11.2658227848101267No Hit
GACTTGTCCACAGTGCTTGTGAAAGAAATCTCTCTCTCAAGAGAATTAGA11.2658227848101267No Hit
ATGTAGATGGATTCGAGCCGAACGGCTGCATTGAGGGCAAGCTTTCCCAA11.2658227848101267No Hit
CTATATGCGTCTCCACAACTTGAGGGGTTTTCGGCTGAATCTAGAAAATT11.2658227848101267No Hit
ACCTGGCATCAATCCGGGCCCTAGACACAATGGCCTCACCCAAGGTAGAA11.2658227848101267No Hit
CACCATGGACACAGTAAACAGAACACACCAATACTCAGAAAAGGGAAAGT11.2658227848101267No Hit
GCTAGGGAAAAATTTCTCGAATAGATTGCAGCACTTCTGGTACATCTGTT11.2658227848101267No Hit
GGTACGGACAAGTCAACAGGCAGGATCAAGACAACAGGAAAGGTACTAGA11.2658227848101267No Hit
GGGAGCAAGTGAGTCCGGGCGGAAGGCTGTCCCCGAGGGGGGTGCAGCTT11.2658227848101267No Hit
CTTGTGACCATTGAATTTTCACAATTTCCCAGTTCCTGATTATCCATTGA11.2658227848101267No Hit
GTATGACAGTGATGAGCCAGAGCCCAGATCTCTAGCAAGCTGGGTCCAAA11.2658227848101267No Hit
GTACATAAATACGGCCTTGCTCAATGCATCCTGTGCAGCCATGGATGACT11.2658227848101267No Hit
CTGGAGAATTGCCTCTTACCAGTATCCTCAACCCTGATCCTCTCACATTC11.2658227848101267No Hit
TGCTAGGACTGCCAGGACTATGTTGAATAACAGTAGTGAGAGTTTACACC11.2658227848101267No Hit
CCTTAGTAGTATCAAGGTCTCTAATCGGTTAAAGATTACACTGAAGTTCG11.2658227848101267No Hit
CATCTGTACCTACTTCGCGCTACCTTTCTGACATGACCCTCGAGGAAATG11.2658227848101267No Hit
TTCACGATGCGAGCAAAATCGTCGGATGATTGGAGCCCCTCCCACCCGTG11.2658227848101267No Hit
GGAACTATGCGCAAGCTTGCCGACCAAAGTCTCCCACCGAACTTCCCCAG11.2658227848101267No Hit
TTGTAGAATCTGGTGACCCGAATGCACTATTGAAGCACCGATTTGAGATA11.2658227848101267No Hit
GGTCATGGGGCTCCACCAAGTAGAGAAGTCAGCGAGGAATGCGCGGGCTC11.2658227848101267No Hit
ATATAGTCCTATTCCCTCCCCCTCGTGACTCGCGCCTTCAATCCTTATTT11.2658227848101267No Hit
GCCTTCAACATTCACTCCAGCATTCCAAAGCACTACACACTAAGCTCAAC11.2658227848101267No Hit
GGTCCGGGGGCTCAAGGCGGGGCGGGCAGCCGGCATGCGTGCAGGAGTTG11.2658227848101267No Hit
GTACCATGGAGTATGTTTCTGCTCGCTTAAGTACCCTCTACATCCAACAC11.2658227848101267No Hit
ACTCTGTTCTGGTGGGGCAGCAGCAAAGGGGAGAAGTTTTATTATTTGGA11.2658227848101267No Hit
GCATAAATAAATACACTATAGATACCCCATGTTATTTTGCATGAATAATC11.2658227848101267No Hit
CCCAATTACAGCAGACAAGAGAATAATGGACATGATTCCAGAGAGGAATG11.2658227848101267No Hit
CCTTCCTAGGACTCGGGGGACGCAGGCCCGGACCCAAGGGAAGTAGCCCA11.2658227848101267No Hit
ACCCAGGACATTCCTCTTTTACATCCAGACTATACGAAGACTGACAAGAA11.2658227848101267No Hit
GTGCAGGAGGAGCCTGCCAAGGTCACGGGACTGTCCTCCTTCCTGACCTT11.2658227848101267No Hit
ATACTGACATCAGAGTCACAGCTGGCAATAACAAAAGAGAAGAAAGAAGA11.2658227848101267No Hit
CACTAAGACCACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAG11.2658227848101267No Hit
GCCCTGCAGTGGGCCACTTCTGCTGTAAAATAGTTCCTCCTCATGCTTGC11.2658227848101267No Hit
CTTATCCCTCTCAGCGACATCTCCGTGCTTGGGGTCATGTCGGGCAGTAT11.2658227848101267No Hit
GAGCAGAAATGAAAAGTGGCGAGAGCAATTGGGACAGAAATTTGAGGAAA11.2658227848101267No Hit
CAAAAATATATAAGAGTCTAATCTCAGGGCCTTAACATATTCAGGAGTAA11.2658227848101267No Hit
CAGTGATGGAATTGACCTCAGTTAGGCAGCATAGTAAGCTTTGGTGTACT11.2658227848101267No Hit
CTTTTGTTTCTGCCTGCAAGGACCCTGTGGGCTTGGCAGCCTGTGGCCAG11.2658227848101267No Hit
GAGATATCTTTACATTGCTTGGCAGTGGGAACTGATTTCTCTACTAAATC11.2658227848101267No Hit
AAAGCAGGGGGAGAGGGAGAAGCAGGCTTCCCGCTGAGCAGGGAGCCCGA11.2658227848101267No Hit
GTGGGGGCAAGAATACTGACATCAGAGTCACAGCTGGCAATAACAAAAGA11.2658227848101267No Hit
CCATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGAGAAGCACC11.2658227848101267No Hit
TGTCCAAAATATGTAAAAAGCACAAAATTGAGACTGGCCACAGGATTGAG11.2658227848101267No Hit
GGGAAGGGCTCTCCCTGAGCTTTGACCTGAGCTTCGTGGCTGGGCTGCAT11.2658227848101267No Hit
GAAATGTGTAGTCATCTTTTCAATAACAGAATTTAATTTGTTAGTACTCT11.2658227848101267No Hit
CTTTATCACTGTTACTCCAATACTCATGTCAGCTGATTCATTTACTCCAG11.2658227848101267No Hit
CTATTAGAAGAGGCCTTATTTTCTCAATTTTCTTCTTTGTTGATTCATTG11.2658227848101267No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGTCCA50.0145.04
ACTTGTC50.0145.02
TGTCCAC50.0145.05
GCAAAAG50.0145.0145
CCACAGT50.0145.08
GTCCACA50.0145.06
TCCACAG50.0145.07
CTTGTCC50.0145.03
GACTTGT50.0145.01
CACAGTG100.072.59
Produced by FastQC (version 0.11.5)