FastQCFastQC Report
Fri 2 Oct 2020
HTGTLBGXG_n02_S2_R2_A03_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTGTLBGXG_n02_S2_R2_A03_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGAAACAAGGATGTTTTTTATCATTAAATAAGATGAAACGATAAAGCT12.7777777777777777No Hit
CAATTGAAGAGCCAGCTGGGCCGTTGGAGGTCCAAGGTGAGTGTGTATGA12.7777777777777777No Hit
GGATAATCAGGAACTGGGAAATTGTGAAAATTCAATGGTCACAAGATCCC12.7777777777777777No Hit
AGCATTTCTGCTGGTATTTGTGTTCGAATCTTCATTCTTTTACTCTCGAA12.7777777777777777No Hit
CCAATACTCATTTCAGCTGATTCATTTACTCCAGACACTCCAAAGCAGGT12.7777777777777777No Hit
CTATTAGATCTACGAACGTCGGGTCGCGATGTGGACCCTGAACAATAACC12.7777777777777777No Hit
CTACTTCATCTTTCTACACAGATGATCCCATTGTCCTTTTGAAACTGCTC12.7777777777777777No Hit
GGGGACGGGGAGGGCGGCTGTTGGATGCTGTCTGTGGATGTCGGCGTTGG12.7777777777777777No Hit
ATCTCCTGGTTGCCTTTCTGAGAATAGCTTTTGCTCTTATCCCAACCATT12.7777777777777777No Hit
AAATTAAAAAGGAACTGCCATGTGCATGAACTCTCTTGAATTGTGACTAA12.7777777777777777No Hit
GAATCTACAGGACCAGCAAGTTAGTGGGAATCAACATCACCAAAAAGAAT12.7777777777777777No Hit
TTTGAGGATATTGCACATTCTTTCATAAGCAACCCTTGTCCTTCGTCCAT12.7777777777777777No Hit
CGTTCCAGCCAAAGAATGTCTTCCAGCATTTGATTGCATCAGATACTGGT12.7777777777777777No Hit
AATATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAAGGTCGCA12.7777777777777777No Hit
CTCACTAAGCCCACTGTGGACCATATGGCCATAATCAAAAAGTACACATC12.7777777777777777No Hit
GCCCAGATCGGCATGATCTCGGAGAACTCTGCTTTCGTGATCCGTCAAGC12.7777777777777777No Hit
ACATTAGCCTTATCACCTTTTGCAAGATAGCTAAGTTCATTGATTCTTAT12.7777777777777777No Hit
GCACCTGCGGCACCAGACCTTCTGGGAAGTGTTGAACCTTGCATTAGAGA12.7777777777777777No Hit
GGAGGAGTAAGGATGGTCGACATCCTTAGACAGAATCCAACTGAGGAACA12.7777777777777777No Hit
GGCTATGGCTTTCCTTTAATAATCCAACCCAGTAATATTTGAGCAATAAT12.7777777777777777No Hit
GTGCTAGACAGAAAAATTCAGTTATCATTCCTTATTTGTGGATTCCTCGA12.7777777777777777No Hit
GTGTTAATATTGAAGTGTTTCACTTAACCCAAGGGCCGTGCGGGGAGCAG12.7777777777777777No Hit
GGGAATTTTAGGATTTGTGTTCACGCTAACCGTGCCCAGTGAGCGAGGAC12.7777777777777777No Hit
GCAGATATTTTATTAGATCGAAACGATACACAGTCGCTTTCAATCTCTGC12.7777777777777777No Hit
GTCTCAAATGTCCCAAGCACATCCAGCATTTGCTGGAACAGGGTCCGTAC12.7777777777777777No Hit
GCTAGCACCTGCTTCCAAGCAATGAGGTAATTGGGATTTATGCCTTTCTA12.7777777777777777No Hit
AACTTTGACCTCTGATGGCAAAGAGGCCCATCAGGCAATCTGAGGGGGCC12.7777777777777777No Hit
GAACTAGAAATGGCAGAATTGGGGTAGTGGACTACAATCGAAAAAAACTA12.7777777777777777No Hit
GAGTTGAACAAAGATGAATTAAGCAAAACCCATGCATCATTAAACAGGCT12.7777777777777777No Hit
CCTTTCGTCAGTCCGAAAGCGGCGAAGAGACAATTGAAGAAAAATTTGAG12.7777777777777777No Hit
GTACTCACTGGTCGGGATAGACCCATTCAAATTACTCCAACACCGCCAAG12.7777777777777777No Hit
ACCTGCATCCTTTCCAAGAACTGTAAGTCGTTTGGTTGCCTTGTTGTAAT12.7777777777777777No Hit
CGATAAACAATGGACACAGTAAACAGACCACAACAATACTCAGAAAAGGG12.7777777777777777No Hit
CCCCTCAACACTTGGCCTATCGCACACCTAAATAGCATAAATCATAATTC12.7777777777777777No Hit
TCATTACCCTAAGGTATATAAAACTTATTTCGAAAAGGTCGAAAGGTTGA12.7777777777777777No Hit
GATGTAGGACCATAATTATAAAATATACGGAATATTCACATTCATGAAGT12.7777777777777777No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)