FastQCFastQC Report
Fri 2 Oct 2020
HTGTLBGXG_n02_S2_R2_A03-FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTGTLBGXG_n02_S2_R2_A03-FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1339989
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA3400.046.960511
CAATTCG2750.039.512689
GTACGTC750.001483350438.667812
GTTACGC1656.051596E-835.130253
ATTCGAC2950.031.9823511
GCGAAAG4800.031.719692
CGAAAGC5050.031.5650543
GTAGAAA14300.031.466231
TAGAAAC15850.027.9030342
CAGCTCG7500.026.0783739
TTACGCA2259.1745096E-725.7573784
GAGTTAT8500.025.567037
ATAGACC8200.024.7411213
CCTTATA5300.024.6233712
AGTAGAA16850.023.689281
GTATTAC7800.023.2613261
TACGCAG2502.2966688E-623.1807755
GTATTAA4700.023.162341
CCGTGCG1600.002186040622.6374749
TATACTT13800.022.571865
Produced by FastQC (version 0.11.5)