FastQCFastQC Report
Fri 2 Oct 2020
HTGTLBGXG_n02_S2_R2_A02-FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTGTLBGXG_n02_S2_R2_A02-FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1195742
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAGACATCTTAACAATATTGTTCTTCCAATCCATGAGCATGGATTGT23500.19653068973072788No Hit
ATGTAATACCTATCAAAATACCAATAACACTTCTCACAGAACTAGAACAA22070.18457158818541122No Hit
GTATTACATCAAATATGTAGATTTTGGGTAGTATAGACATCTTAACAATA19200.1605697550140415No Hit
GTTTATATGAGAAAGGAGGGGAATGAAAGAGAAAATAGAATCAAAACACC14340.11992553577611223No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA2900.087.552421
GCGAAAG4500.057.995882
CGAAAGC4900.053.2214243
TTACATC11100.034.5828174
TATATAC6950.034.39613
GTATTAC12850.033.872421
TATACAG3600.032.1902925
CGTATAC1153.1777885E-431.4957713
ACCTATC10900.031.2331038
TACATCA11600.030.5946525
TATTACA14700.030.0828972
GTGCTAG2853.2196112E-1027.9992221
GGTATAG1907.2076127E-626.6862899
TAATACC13300.026.6840574
GTAATAC14500.025.4789943
CCTATCA14100.025.1721739
CTAATCG2351.3423432E-624.6563934
TGTAATA15300.024.6387712
ACATCAA14500.024.4757216
TATAGAC14300.023.8269792
Produced by FastQC (version 0.11.5)