FastQCFastQC Report
Fri 2 Oct 2020
HTGTLBGXG_n02_S2_R2_A01_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTGTLBGXG_n02_S2_R2_A01_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCATCAAGATACAGCAAGAAGTTCAAGCCGGAAATAGCAATAAGACCCA212.5No Hit
TTTGCCGTCTGAGTTCTTCAATGGTGGAACAGATCTTCATGATCTCAGAG16.25No Hit
GTGCATGCTAGCGAGGGGCAGGGGGCGGCCGTGGGTGGATCTGGGATGAT16.25No Hit
GATGTCAATACGACTATACTGGTACGAACCAGTACCGCTCCCCCTTCCAT16.25No Hit
CCGAACTTCCCCAGCCTTGAAAACTTTAGAGCCTATGTAGATGGATTCGA16.25No Hit
GTGAGTGACAACTCAATACTTACAAAGTTCACAACATCAGTATAATTTCT16.25No Hit
GTAGAAACAAGGCATTTTTTCATGAAGGACAAGTTAAATTCATTATTTTT16.25No Hit
GTTTTAAACATTTCGACACTAATTGATGGCAATCCGAATTCTTTTGGTCG16.25No Hit
CTGCAAACATACTAACACAACAATAATAATTAACACCAAAATAACTAAGA16.25No Hit
GAACCTTGCCTCTCACTAAATGACATCTCTCTTACATACTTCAATCTTCT16.25No Hit
GGACTGTGTGGTCTGCCTGGAACCCTGATGTGCAGGGACAGTCCGAGGTG16.25No Hit
AAATTAATGAAAGAGACATCAAAAATGAAAGTGTAACCAGGATGAATATG16.25No Hit
ATGAATATCTGTTACACAAATGCATAGCTATGAATGGTGCCATGATCTCT16.25No Hit
GATTTCCCTTTCATCGACGAACGGGGTGAATCAATAAGTGTAGAAGCTTG16.25No Hit
GGGTTGGAGGATGATGTCATTAGAGGCTTTCGCATGTTGTGCAAAGAAGC16.25No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)