FastQCFastQC Report
Fri 2 Oct 2020
HTGTLBGXG_n02_S2_R1_H11-FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTGTLBGXG_n02_S2_R1_H11-FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1001702
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATATAAAGAGCTTAAGGGAAATATGGTAGAAGGAATTGGTAAAAAGAAG20580.20545032354931905No Hit
GTATATATAGCTACAGTACCTCGATAAGAAGCAGTTAAGTGGTGAGACTG12780.12758285398252175No Hit
GTCATTGATTAGTCCCAGTTTTTATTTGATTGGTGGCAGCATTTTTCTTT10060.1004290697233309No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA3900.085.5345151
GCGAAAG5500.060.624482
CATCGTG502.0077257E-458.0147171
TAGGATA651.1007622E-555.70824
CCGTTAG1201.9897197E-742.2474949
ATAGGAT1601.020453E-940.742723
ATACGGA1604.6360583E-836.2085236
GATATAA9650.036.0713231
CGAAAGC9850.035.2965663
TAAGGTG2501.8189894E-1234.7619175
CGAGCGA1551.4632496E-632.746967145
TATAAAG12450.032.579633
GTCATAT950.004721433430.5340631
TGCCGAT950.004751524430.494439
GTATAGA4050.030.439821
ATATAAA12650.029.223522
GGATTAG1753.767169E-629.0073591
TATTTAG3500.028.9943142
TACTCGG1255.1869807E-428.9682647
ACCGTAT1504.4316846E-528.9682628
Produced by FastQC (version 0.11.5)