FastQCFastQC Report
Fri 2 Oct 2020
HTGTLBGXG_n02_S2_R1_H10-FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTGTLBGXG_n02_S2_R1_H10-FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences475031
Sequences flagged as poor quality0
Sequence length151
%GC47

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGCAACACCGCTCATGGATTATTCATGACACTAGCCAGACTCCGAGCTC20940.4408133363927828No Hit
GTCTCATATCTATCCCTGTCTCTCTCTTTGTCTCTCACACTCTCTGTCTG19130.4027105599424038No Hit
ATTCATGACACTAGCCAGACTCCGAGCTCAACCAGAGGGCACAAACAATT15180.31955809199820645No Hit
CTGTAACACTGTGGTGGTTTCCACAGCAACGGCCCAGAGACAGAGGGTGA14860.3128216895318411No Hit
CCGTAGAGATGCTGGCGAAAGCAGTCAAGCGAAGTTGTCAAAGCTCAAGT13400.28208685327904914No Hit
ATCTATCCCTGTCTCTCTCTTTGTCTCTCACACTCTCTGTCTGTCTCACA12020.25303611764284856No Hit
GTCCAAAGAACATCGCTTTTCAGTGACGTTGTCAGGGGAGAAGGGGGAGA11260.23703716178523085No Hit
CACACACACCGTAGAGATGCTGGCGAAAGCAGTCAAGCGAAGTTGTCAAA11190.23556357374571343No Hit
GTACAGGAATGAGGAATGGATGCTGGAATATTTGTCTCCCCCTCCTCTGC10980.23114280962716116No Hit
TATCTATCCCTGTCTCTCTCTTTGTCTCTCACACTCTCTGTCTGTCTCAC10740.22609050777738718No Hit
ATAAAATATTTTCCAGTAATAAAATGCAACACCGCTCATGGATTATTCAT10410.2191435927339479No Hit
GGCTTAATTAACATATAAAATATTTTCCAGTAATAAAATGCAACACCGCT9840.2071443758407346No Hit
CTATTTGACAGATTCAGGGTAATGTCCTCTTAAGAAAATAAAATTCTTCA8720.18356696720845586No Hit
CACACACACACCGTAGAGATGCTGGCGAAAGCAGTCAAGCGAAGTTGTCA8620.1814618414377167No Hit
CTCATGGATTATTCATGACACTAGCCAGACTCCGAGCTCAACCAGAGGGC8560.1801987659752732No Hit
CACACACACACACCGTAGAGATGCTGGCGAAAGCAGTCAAGCGAAGTTGT8220.17304133835476No Hit
ACACTAGCCAGACTCCGAGCTCAACCAGAGGGCACAAACAATTATGCCTC6870.14462214044978117No Hit
GGATTATTCATGACACTAGCCAGACTCCGAGCTCAACCAGAGGGCACAAA6680.14062240148537675No Hit
GTTGGTAATGGCTTAATTAACATATAAAATATTTTCCAGTAATAAAATGC6390.13451753675023315No Hit
GAGTAGGAGTGACGTGGAAGCAGGCATCGGAGCAGGTGCAGGAGGTGGGA6350.13367548644193747No Hit
GATCAAACCATGTTGTTGGTAATGGCTTAATTAACATATAAAATATTTTC6320.13304394871071573No Hit
GGGTGAGAATGTGCTGTGGCAGTGACCTTGTACAAACACACACATACACG6260.13178087324827223No Hit
GAGTAATAGTAGCGTGTGTGTGTGTGTGTCTGTGTGTCAGTTATCAGATT6210.13072831036290264No Hit
CAATTATGCCTCACTGAGTAATAGTAGCGTGTGTGTGTGTGTGTCTGTGT6190.13030728520875481No Hit
GTGTAGGAATGGGTGTGAGCGTAGGAGGTGGGAACAGGCAGGGGAGCCGG6180.1300967726316809No Hit
GTAATAAAATGCAACACCGCTCATGGATTATTCATGACACTAGCCAGACT6080.1279916468609417No Hit
GTTGTTGGTAATGGCTTAATTAACATATAAAATATTTTCCAGTAATAAAA5960.1254654959360547No Hit
ATCTGTAACACTGTGGTGGTTTCCACAGCAACGGCCCAGAGACAGAGGGT5950.12525498335898078No Hit
CTATAGGAGACTGTAAGCCGCAGCTGAATCTGGGCTCCTCTGTGTGAGGC5940.12504447078190686No Hit
CATCAAGAGGTTCTGTGATGCTGGGCAATTCATCTAAGCCTTAATTTGCT5860.12336037016531552No Hit
CTTTATAATCTTCGCCAGGGACTTCAGAAGCCTGTGTCACGTAGTAATTT5660.11915011862383718No Hit
ATTCAGGGTAATGTCCTCTTAAGAAAATAAAATTCTTCAATCAGAACATG5630.11851858089261542No Hit
CACATACACGCACACACATACACATGCACACACACATACACACACACACA5500.1157819173906545No Hit
GTTGGGAACCATCTGTCAGATCATCCAGCAAAACACGCTGACATCCTTCA5490.11557140481358058No Hit
TTCCATACCCGGGTGAAGAGGGATGTAGTGGGCGCTTGAGCAGAGGAGAG5490.11557140481358058No Hit
ACCATGTTGTTGGTAATGGCTTAATTAACATATAAAATATTTTCCAGTAA5260.1107296155408805No Hit
ATATTTTCCAGTAATAAAATGCAACACCGCTCATGGATTATTCATGACAC5260.1107296155408805No Hit
CCAAAGAACATCGCTTTTCAGTGACGTTGTCAGGGGAGAAGGGGGAGAAG5190.10925602750136305No Hit
GTACACAGAAAAGCAAATTAGAGAAACAGGATGTAAAGCACAATTCTATT5120.10778243946184564No Hit
ATGTTATTTCTACCCTCATTTGACAGATTGGGACTTAGAGGTTTAGAAAT5120.10778243946184564No Hit
GTAGAGATGCTGGCGAAAGCAGTCAAGCGAAGTTGTCAAAGCTCAAGTTG5010.10546680111403256No Hit
CATAAGCACACACACTCACACTTAAGATGTCAGAAATGGCTGTGGATAGA5000.10525628853695863No Hit
CATACACGCACACACATACACATGCACACACACATACACACACACACACA5000.10525628853695863No Hit
GCCTTTACCTGGACTTCTTTGTGGCTCTTGCCATCTGTAACACTGTGGTG4850.10209859988084989No Hit
TTATTTACCCAAGTGTGTAGGTCTGCTTTTAGATGGGAAGGGAGATACTG4820.10146706214962814No Hit
GTTAATGAGTTTTTAAAAAGCCTTCCAAAGTGATTATAAGATCCTTTTTT4800.1010460369954803No Hit
CACTTAAGATGTCAGAAATGGCTGTGGATAGATTTTGCACGGCATAGTGT4770.10041449926425855No Hit
GGTTTAGACTCTTAAGTCAGAGGGCCTCTGTGTGTGTCGGGCACCCTTGC4760.10020398668718464No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATTTGC100.0070817056145.0547145
AGCGAAA3700.0100.117871
GTCTCAT4400.072.634521
TGCAATG300.001941933672.435745
TCATATC4850.067.215494
AACGTTC606.8359604E-660.3631177
CTCATAT5550.058.7564163
CATATCT5550.058.731685
TCTCATA5600.058.305492
TAACCCC1251.8189894E-1257.948595
AGTAACC1301.8189894E-1255.7432673
GAAACGT651.0985208E-555.71985
AGTATTG553.2313954E-452.6805345
AAACGTT701.7035418E-551.739816
CCTGGCC901.31866E-648.351566145
GGAAACG803.748936E-545.27714
ACGTTCT803.751268E-545.2723358
ATATCTA7300.044.6521646
CTATTTG2600.041.9045331
GACAAGT700.001046489541.5054441
Produced by FastQC (version 0.11.5)