FastQCFastQC Report
Fri 2 Oct 2020
HTGTLBGXG_n02_S2_R1_H05_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTGTLBGXG_n02_S2_R1_H05_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTCCAAACACTGAAAATAAGAGAACATGAAGGGTATGAAGCATTCACAA15.0No Hit
TGATTAGTATATGTGAAGACTTGGGACGCAAATGACGACCTGGGGAAGTC15.0No Hit
GTGTCCTTACGAATCCACTGTACCGGCTTCTGGTTGCCTTAGGGACAAGA15.0No Hit
CATGTACTGATCCAAAATGGAAGACTTTGTGCGACAATGCTTCAATCCAA15.0No Hit
GGAGGGAGCAATAGTTGGAGAAATTTCACAATTACCTTCTCTTCCAGGCC15.0No Hit
CTATTCTCTGTCGCTTTCAATCTGTGCCGCATTTCTTCAATTAACCACCT15.0No Hit
CCACAAACCTGCTCCTCTCACATTCACAGACAAAGCGCAAAATTGCATCC15.0No Hit
CAGCAGAAGTGTCCCACTGCAGGGCTACTGAATACATAATGAAGGGAGTG15.0No Hit
ATATCATGAAATCAAATGCATGAAGAGATACTTTGGCTGCATGGAGCCTA15.0No Hit
TCATGGGACAGCCCAAAAGCAATCGTTAGATCAAATGGCCTACCAGCTAA15.0No Hit
GTTACATATACTGTTCACCACTGTCCAGGCCATGATTCGGTCTCTTCCTT15.0No Hit
GAACTTTGTCAATAGGGCAAACCAGCGACTGAACCCCATGCACCAACTCT15.0No Hit
GCTAATGAGTCAGGAAGGCTAATAGATTTCTTAAAGGATGGAATGGAATC15.0No Hit
ATGGGGGGCCGGGTATTCTCAGTGGAGCATTGCTTGATGAGGGATGTTAG15.0No Hit
CATCATCCATTAGCTCCCCTTTTAAGCAGACTTCAGGAATGTGAAGATTC15.0No Hit
GTTTCAAGGCATGAATTCTCAAATATTCCTGGGTGGGATTCTTCAAGGAA15.0No Hit
ACTTAGTGTTGTCCCCAGTGATTGTGAAAGAAATCTCTGTGTCTTGTGAA15.0No Hit
CGGTTAAAGATTACACTGAAGTTCGCTTTCAGTACTATGTTCTTTTCCAT15.0No Hit
CATTTGAGTTAAAGAAGCTGTGGGATCAAACCCAATCAAAGGTAGGGCTA15.0No Hit
CACATATAGGGACATGGGCCTCGACACTTGGCCTATCGCAGTCCTCAAGC15.0No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)