FastQCFastQC Report
Fri 2 Oct 2020
HTGTLBGXG_n02_S2_R1_H04_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTGTLBGXG_n02_S2_R1_H04_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences990084
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATATAAAGAGCTTAAGGGAAATATGGTAGAAGGAATTGGTAAAAAGAAG20450.20654813126967006No Hit
GTATATATAGCTACAGTACCTCGATAAGAAGCAGTTAAGTGGTGAGACTG12700.12827194460268018No Hit
GTCATTGATTAGTCCCAGTTTTTATTTGATTGGTGGCAGCATTTTTCTTT9990.10090052965202952No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA4150.064.6818851
ACGTGAA400.006044516354.38943145
GCGAAAG5400.049.6665042
GCATAAC450.0096463348.324172
CCTATAC2200.046.10433
GAGCGAA1053.8065027E-641.456341
CGAGCGA1601.0077201E-940.792076145
GTCATAT907.452885E-540.3047751
GATATAA9050.039.2804531
TGCCGAT1406.664013E-736.2120069
TATAAAG11300.034.6219443
ATACGGA1051.864607E-434.4893656
AACTTGC4600.033.0614668
ACCGTAT1102.4519025E-432.9183438
ATACTGC3600.032.1900756
TAAAGAG13850.031.3781935
ATAGGAT1204.0745735E-430.1873463
TTATATC6250.030.1390463
CCGCAAA1000.00606655429.007696145
TATACTG5000.028.972535
Produced by FastQC (version 0.11.5)