FastQCFastQC Report
Fri 2 Oct 2020
HTGTLBGXG_n02_S2_R1_H01_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTGTLBGXG_n02_S2_R1_H01_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences52
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTATAATGTTGTTCTTTATCACTGTTACTCCAATACTCATGTCAGCTGAT11.9230769230769231No Hit
GACTATGTTAGGCTCTTTCCAGCCAAAGAATGTCTTCATGCATTTGATTG11.9230769230769231No Hit
GAAGAGAACGCTTCGTGGTGCGAATCCATGAATGGCTAGTTGCACCAGTA11.9230769230769231No Hit
CTCATAATTAGTGCCAAACATAAACACAGATTTAACTTTCATTCCCATTA11.9230769230769231No Hit
CGATTGGACCAGGCGATCATGGAAAAGAAAATAGTACTGAAAGCGAACTT11.9230769230769231No Hit
GTACTCTCATTATGCAAGCATGAACATTTAAATAATAATTATATAACAAA11.9230769230769231No Hit
TCCCAAAGTTTGATTATAGCTGCTAGAAAAATAGTAAGAAGAGCAGCAGT11.9230769230769231No Hit
CAATAGCCTTTGCCATAGTGCTAGCCACCAACATTAAGTTTTCATCCCTG11.9230769230769231No Hit
GTTCTATGGCAATCTTGGACTGGTTTTTCTCAAATGATAATAACAGATCC11.9230769230769231No Hit
GGAAGGCACGATCGGGTTCGTTGCCTTTTCGTCCGAGAGCTCGAAGCCTC11.9230769230769231No Hit
AATAGAGATAGAACCATACCATCTGGTGGGGAAACCAAGAGTTGAATGGC11.9230769230769231No Hit
CTAACAAGAACTTACTTACCATTATGAACCAGACAATCATCATCCAAGGA11.9230769230769231No Hit
ATCCAAACGCCATTGCCGTATTTGAATGATACCCCTTTTACTGCCTTTGC11.9230769230769231No Hit
GGTATTATCATTTCAGATACACCAGTCCACTATTGCAATACAACTTGTCA11.9230769230769231No Hit
CCACCCCAGCTGTGCCTTCATCTGGATCTTCAGTCAATGCCCCTGCATCC11.9230769230769231No Hit
ATGCAATGGGGCTACCCCTCTTAGTTTGCATAGTTTCCCGTTATGCTTGT11.9230769230769231No Hit
CCCCAAGACTCAGTGGCTGCTTTGCTTTCACTATTTCGCTCAAAGATATC11.9230769230769231No Hit
CATTAGGGTGCAATGAAAAGAATTCCAACCTGTCAAATTTTGTGAGAAAG11.9230769230769231No Hit
TTCTATAAGAGACCAAAATATAAGCATTATTTTATAACTTGAAACATGTA11.9230769230769231No Hit
CCTTTGCCCCAGGGAACGTTATTTCTCAATTGAGCTTCTTTTATAGTTTA11.9230769230769231No Hit
ATGTAGATGGATTCGAGCCGAACGGCTGCATTGAGGGCAAGCTTTCCCAA11.9230769230769231No Hit
TCTATAAGAAGCAGTTAAGTGGTGAGACTGGATAAAAGGTAGAAACTCTG11.9230769230769231No Hit
GCTAGATGCAGTAAAGACCACAGATTAAGATTCGGTTCCAAGGGAACAGA11.9230769230769231No Hit
CCATTACTTGGTCCATCGGTCATTACAGTAAAGCAAGAACCATTTACACA11.9230769230769231No Hit
CACCGGAATGGATCCCAGAATGTGCTCTGTAATGCAAGGTTCAACACTTC11.9230769230769231No Hit
CTTGTGACCATTGAATTTTCACAATTTCCCAGTTCCTGATTATCCATTGA11.9230769230769231No Hit
GGTATTATCATTTCAGATACACAAGTCCACGATTGCAATACAACTTGTCA11.9230769230769231No Hit
CAACAATAATAACCCATTACCAAATAGCACAATAGACTAATCTCCAAATG11.9230769230769231No Hit
ACAATAAAAAGAAAATTAGACCGCAGATAGAAAATCCACAAATCATGCAA11.9230769230769231No Hit
CGGATGACGACACCAAATGCAGTTATCACCTCCACAAAAGGATGGCCTGG11.9230769230769231No Hit
ACATATGCATCTGCATTCTGATAGAGACTTTGTTGGTCAGCACTAGTAGA11.9230769230769231No Hit
CATAATGGACTCCAACACCATGTCAAGCTTTCAGGTAGACTGTTTCCTTT11.9230769230769231No Hit
CATTAGAGCCCATCCAGAAAATGATTGCCCCCAGGGAGACTACCAGTACC11.9230769230769231No Hit
CTTCTTGTATAGTTTAACTTCTCTATCCATGTTGTTCGGGTCCCCATTCC11.9230769230769231No Hit
GTGCCAACGCAGTGAGGACTAATCAAAGTGGTGTGCAGAGCCAGCTTTAG11.9230769230769231No Hit
CGTATTATGACACTTCTGGTGGTATTACTATGTGTATAGCAGATAGAATC11.9230769230769231No Hit
AATGTATCCTATCTGATATTCCAGATTCTGTTAGAAAGACACCCACGCTC11.9230769230769231No Hit
CTTTGCGGTAGAGTGTGTCATTTGGGATATCCGCAAGCGAGTTGCAGCCA11.9230769230769231No Hit
CATTATGATGGACGACTAATCCAGAATAGAATAACAATAGAGAGGATGGT11.9230769230769231No Hit
AGTTCAGGCAATGGAACGTCTTCCCCAGGAGATTTCATCGATTATGAGGC11.9230769230769231No Hit
GAGATGTCCTGACCCAACAGCAGGGGAGTTAATGCAGAAAGGGCTGGGCT11.9230769230769231No Hit
GAACAAAGAGGAAGACAGGGGAAAGTGAAAAAGAAGGGCTCGAGCCCCAC11.9230769230769231No Hit
TCTTCAATTAACCACCTTATTTCCTCAAATTTCTGTCCCAATTGCTCTCG11.9230769230769231No Hit
CATTAGAGCACATCCAGAAACTGATTGCCCCCAGGGAGACTACCAGTACC11.9230769230769231No Hit
CTCTAGCTCTATGTTGACAAAATGACCATCGTCAACATCCACAGCACTCT11.9230769230769231No Hit
CCTCTGGGCAGCTGTTTGAAATTTTCATTTGAGGATATTGCAAATTCGTT11.9230769230769231No Hit
CAATACTAGTAGTTCTGCTATATACATTTGCAACCGCAAATGCAGACACA11.9230769230769231No Hit
TTTCTGCTCTGGAGGTACTGAAGGTCTGCCGTTCTAATCACAGTTTCTCC11.9230769230769231No Hit
GCACAGGAGCTTCGATGAGTGCCAATAGTTCTCATTGCATCTACCATATG11.9230769230769231No Hit
GTATAACATAGTTGGAACTTGTGACCTGTGAATATTCAATATTTCGGAGA11.9230769230769231No Hit
TCTTTCTTCTCTTTTGTTATTGCCAGCTGTGACTCTGATGTCAGTATTCT11.9230769230769231No Hit
ATGATTTAGCCTGTGATGTTACGAGAGCTGGAGCAACCAAAGGAAATGCA11.9230769230769231No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTTGTT50.0145.07
TTGTTCT50.0145.09
TATAATG50.0145.02
TAATGTT50.0145.04
TTATAAT50.0145.01
ATAATGT50.0145.03
ATGTTGT50.0145.06
CTGTCTT50.0145.0145
AATGTTG50.0145.05
GTTGTTC50.0145.08
Produced by FastQC (version 0.11.5)