FastQCFastQC Report
Fri 2 Oct 2020
HTGTLBGXG_n02_S2_R1_G09_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTGTLBGXG_n02_S2_R1_G09_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences91
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTCAAATATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATG11.098901098901099No Hit
AGGTAGGGCTATTAGTATCAGATGGAGGACCAAACTTATACAAAATACGG11.098901098901099No Hit
CTACTAGTGTCACCCTAAGACGTCACAGACAACCAAGGAACTGAGAAGAT11.098901098901099No Hit
NNCCTATATTAACTGCTATGGAAAAATTTAGAGAATATATTGCAGCACTT11.098901098901099No Hit
GGAGAGGAGATGGCCACCAAAGCGGACTACACCCTTGACGAAGAGAGCAG11.098901098901099No Hit
TTCTTTTACTCTCGAACATGTACCCTTTCCCTAGTCTTGCCATTTTGTTT11.098901098901099No Hit
TGTCAATCCGACTCTACTTTTCCTAAAAATTCCAGCGCAAAATGCCATAC11.098901098901099No Hit
GCCTTGTTGAAATTGAATACTGGAGAATTGCCTCTTACCAGTATACTCAA11.098901098901099No Hit
TATTTAAGGTCAGTGCTCTTATTAGATAGCCTCTCTTATTCATTCTTTGT11.098901098901099No Hit
ATTCACATCTTTTCATTAACTGGAGAGGAGATGTCCACCAAAGCGGACTA11.098901098901099No Hit
TCTATGGTGTTGGCCATTGCAGTTGCTGCGGGTTGAGTTGTGTTTTATGT11.098901098901099No Hit
GCTAGAAACATAGTAAGAAGAGCAGCAGTGTCAGCAGACCCATTAGCATC11.098901098901099No Hit
AGTCAACACTTATCAATGGATAATCAGGAACTGGGAAATTGTGAAAATTC11.098901098901099No Hit
GTATAACATTGTGGGATCTTGTGACCATTGAATTTTCACAATTTCCCAGT11.098901098901099No Hit
GGTTTATAAAACTTATTTCGAAAAGGTCGAAAGGATGAAACATGCTACCT11.098901098901099No Hit
TCCCTCCACCTCGTGACTCGGGTCTTCAATACTTAATTTCAGAGCATCCA11.098901098901099No Hit
ACTGTGTAAGGTCTCCAACATCTTTGCAGCCATCTACGGCTACTTTTTCT11.098901098901099No Hit
ATCAGGAACTGGGAAATTGTGAAAATTCAATGGTCACAAGATCCCACAAT11.098901098901099No Hit
TCTTTTGCCTAGGCATGAGCATGACCCAGTCTCGTGACATTTCCTCGAGG11.098901098901099No Hit
ATCATATTCCATGCTATTGGCTGGAAAATCGGCTGGCAATACCACATCAA11.098901098901099No Hit
CAATCCGACTCTACATTTCCTAAAAATTCCAGCGCAAAATGCCATAAGCA11.098901098901099No Hit
GATTCTACAGGACCTGCAAGTTAGTGGGAATCAACATGAGCAAAAAGAAG11.098901098901099No Hit
CAAACAGACTGTGTTCTAGAGGCTATGGCTTTCCTTGAAGAATCCCACCC11.098901098901099No Hit
GTACAGGTCATTCCCCGAAGGGAGAGAAAACGTATTTCGAAAAGGGCGAG11.098901098901099No Hit
ACCAGCGACTGAACCCCATGCACCAACTCTTGAGGCATTTCCAAAAAGAT11.098901098901099No Hit
ATTCATATCATGCTCCCCCTTGGAATGCAGAACCTTCTGCTTGACTCAAG11.098901098901099No Hit
AATTGATGGCCATCAGAATTCTTTAGGTCGCTGACAGGCTGTCAGTAAGT11.098901098901099No Hit
GATCAAAGAGGGAACGTCCTATTGTGTCCCGAAGCAGTCAGTGAAACGCA11.098901098901099No Hit
AGGGACAAGAGACTGAAATGGTTCAAATTCCATTTTGTTGTATAACATTG11.098901098901099No Hit
CCTGCATCCTTTCCAAGCACTGTATGTCGTTTGGTTCCCTTGTTGTAATT11.098901098901099No Hit
CACCAAAGAGGACTACACCCTTGACGAAGAGAGCAGGGCAAGAATCAAAC11.098901098901099No Hit
CAGGCAATCTGAGGGGGAGTGGTGTCGTCCTAAAGAATGGTTCAATTATG11.098901098901099No Hit
AGGGAATGGAATCAAGGAACAATGAGGATTGTGAGATAAGCGCCCACTTT11.098901098901099No Hit
ATCCTGATCCTGTTCCATGGCTGTATGGAGGATCTCCAGTATAAGGGAAT11.098901098901099No Hit
AGGCTATGCTGCTCCCGCTAGTCCCGATTGTGTTCTCTTTGGGTCGCCCT11.098901098901099No Hit
ATATAGGACTTCTTTTTGCTCATGTTGATTCCCACTAACTTGCAGGTCCT11.098901098901099No Hit
CTCCAATACTCATGTCAGCTGATTCATTTACTCCAGACACTCCAAAGCTG11.098901098901099No Hit
CTTCTAATAGATGGCACAGCATCACTGAGACCTGGGATGATGATGGGCAT11.098901098901099No Hit
TTCCAAATGTGTGCATATTGCAGCAAACTTGTTAGTTTCGATTTTCGGAT11.098901098901099No Hit
GATGCAGAGATAGGCAAGTAAAAAAGAAGGGATATCGAAACCCCTGGGAT11.098901098901099No Hit
ATTATGTATTCAGTAGCCCTGCAGTGGGACACTTCTGCTGTAAAATAGTT11.098901098901099No Hit
ACAATATACTGGTGGGATTGGCTCCAATCATCCGACGATTTTGATCTCAT11.098901098901099No Hit
CGACAGGCCCCCTCAAAGACGAGATCCCTCAGATACTGCAAAGTGTCTTT11.098901098901099No Hit
GGGGAGATGGTCGAGTGGTTCAGGGGTATGATGCGCGCGCGTGGGCAAGG11.098901098901099No Hit
ATACAGAATTTGCCACAAAGGGCCTGCACCCTATCCCAATAGATCCTACC11.098901098901099No Hit
GGCTCTTCCGTCAATGAACCGGCCTCCTTTCCAAGAACTGTTTACATTAT11.098901098901099No Hit
GTCCATCTGGTCAGCCTCAAGCAGTCCCCTTTGGGTTGTGTTGAGAATAG11.098901098901099No Hit
ATCTCCGTGCTTGGGGTCATGTCGGGCAGTATTCCGAACATTACCATCAC11.098901098901099No Hit
CCCTGGGCTCCAAGACGCGGGAACTGAGATGCAGATAGTGGGCCATAAGG11.098901098901099No Hit
TTTTTGGACAGTATGGATAGCAAATAGTAGCATTGCCACAACTACTTCAG11.098901098901099No Hit
GCCTTTTCGTCCGAGAGCTCGAAGACTCCCCGCCCCTGGAAGGACAAATC11.098901098901099No Hit
GGTCAAATATATTCAATATGGAGAGAATAAAAGATCTGAGAGATCTAATG11.098901098901099No Hit
GGGGTGGAGTCTGCTGTCCTGAGAGGATTTATCATTTTTGGCAAAGAAGA11.098901098901099No Hit
GTAGAAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAACGAGAAAGCT11.098901098901099No Hit
CAGCAGAACAGAGTAGGATGCAAGTTTCCTCATTGACTGTGAAGGTGGGA11.098901098901099No Hit
GTTATGGGAATGATATGTATAATGCACGAAATGACCACAAGCCCTGAGAT11.098901098901099No Hit
GCTCTTCAGGATCTCAGAGAACTCTTCGTTCTTGATCCGTCCAGACTCGA11.098901098901099No Hit
GGTCTAGTGTGTGCCACTTGTGAACAGATTGCTGATTCACAGCATCGGTC11.098901098901099No Hit
GGGAGGGAATACCACTATATGATGCAATCAAATGCATGAAGACATTCTTT11.098901098901099No Hit
GAGCAAGGCCGTATTTATGTACACTCCCTTCATTATGTATTCAGTAGCCC11.098901098901099No Hit
TTCATCTACTCCCATTTTGCTGACTCTTATCCCTCTCAGCGACATCTCCG11.098901098901099No Hit
GCTAATGGGTCTGCTGACACTGCTGCTCTTCTTACTATGTTTCTAGCAGC11.098901098901099No Hit
CATCCCGCATTTGCTGGAACAGTGTCCTTACGAATCCACTGTACCGGCTT11.098901098901099No Hit
GCAGATCTCAGTGCCAAGGAGGCACAGGATGTGATTATGGAAGTTGTTTT11.098901098901099No Hit
CATGTACCCTTTCCCGAGTCTTGCCATTTTGTTTGAGCACATTTTAGGTG11.098901098901099No Hit
CAGTAAGTATGCTAGACTCCCGTTTTGGTTTCCTTAACAAAACAACGTCC11.098901098901099No Hit
GAACAATGAGAAGCAATTTTCTAGATTCAGCAGAAAACCCCTCAACTTGT11.098901098901099No Hit
AAGCAAAACGCAGGGATCATTATTCAGGCACTCCTCGAGTGCTTCATATA11.098901098901099No Hit
CCAGTATATTGTCTTGGTGTATTTCTTTTGTCCAAGATTCAGTATCGAGA11.098901098901099No Hit
TGATACTACTAAGGGCTTTCACTGAGGAGGGAGCAATAGTTGGAGAAATT11.098901098901099No Hit
ATATGTAACACAACAGTTGTAGATAAGCCTAAATTTCTTCCTGATTTGTA11.098901098901099No Hit
GTTTTGCACTTAAACCAAGGGAACAGCTGTCAGCAGATGTCCACTACACT11.098901098901099No Hit
CTACAGATACACATATAGGTGCCATAGGGGAGACACACAAATTCAGACGA11.098901098901099No Hit
TTTATGTACACTCCCTTCATTATGTATTCAGTAGCCCTGCAGTGGGACAC11.098901098901099No Hit
GAAAGATTCAATTTTGGCGATCACTTCTTTTGACATTTGCGAAAGCTTGA11.098901098901099No Hit
GAGAAGAACCCCGCACTCAGAATGAAGTGGATGATGGCAATGAGATACCC11.098901098901099No Hit
CCTATATGTGTATCTGTAGTCTTTGATGAACAATTGAAGAGCCATCTGGG11.098901098901099No Hit
CCTCCCCCATGATAGAAATTCCAACCGGTCTAGTATATGAACTGATAGCG11.098901098901099No Hit
ATTTCGGTCAGTGATTCCACGTTTGATCATTCTGATTAACTCCATTGCTA11.098901098901099No Hit
GGTTGATTTCTGGTGATATATGTAATCATCGCCAGGAACATTCGAGGATT11.098901098901099No Hit
AGTCAACACTTATCAATGGATAATCAGTAACTGGGAAATTGTGAAAATTC11.098901098901099No Hit
ATGCGGGATGTGCTTGGGACATTTGACACTGTCCAAATAATAAAACTTCT11.098901098901099No Hit
CTTCTACACAACTTGAGGGGTTTTCGGCTGAATCTAGAAAATTGCTTCTC11.098901098901099No Hit
TTTGTAAACAATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCG11.098901098901099No Hit
GTTGAACCCAGATGCATTACGCAAAACCCAGGGATCATTAATCAGGAACT11.098901098901099No Hit
AGAAGTTACTTATCAAAAATGGAAGACTTTGTGCGACAATGCTTCAATCC11.098901098901099No Hit
GCACACTTTCCCAATAGATCCTTCCTCCACTCCCCTGGGTGACTCTCCGA11.098901098901099No Hit
GTGTCAGCAGACCCATTAGCATCTCTCTAGGAAATGTGCCACAGCACACA11.098901098901099No Hit
AAACAAGGTCGTTTTTAAACAATTCGCCACTAATTGATGGCCATCCGAAT11.098901098901099No Hit
GTAACACACTCTGTAAACGTTCTAGAAGACAAGCATAACGGGAAACTATG11.098901098901099No Hit
TTCCATGTTCATTCCTCTATGGAATCATGTCAATTATTCTCTTGTCTGGT11.098901098901099No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACACTC50.0145.05
AGCTCAA50.0145.0145
ACACTCT50.0145.06
ACTCTGT50.0145.08
ACACACT50.0145.04
CACTCTG50.0145.07
TAACACA50.0145.02
GTAACAC50.0145.01
AACACAC50.0145.03
CTCTGTA50.0145.09
Produced by FastQC (version 0.11.5)