FastQCFastQC Report
Fri 2 Oct 2020
HTGTLBGXG_n02_S2_R1_G09-FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTGTLBGXG_n02_S2_R1_G09-FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences775159
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAGACATCTTAACAATATTGTTCTTCCAATCCATGAGCATGGATTGT22980.29645530787877067No Hit
GTATTACATCAAATATGTAGATTTTGGGTAGTATAGACATCTTAACAATA17840.23014633126881065No Hit
ATGTAATACCTATCAAAATACCAATAACACTTCTCACAGAACTAGAACAA15180.195830790844201No Hit
GTTTATATGAGAAAGGAGGGGAATGAAAGAGAAAATAGAATCAAAACACC14260.18396225806576455No Hit
TCTCTATATTCCCCCATATCACAAGCCAAGGATGTTACTCCACCTGTCCC12540.16177326200173126No Hit
CCTTAGTACAACTTTGTAGTATAACTTGAAGTCTGGAACTTGATACCTCC9870.12732871578605165No Hit
GTCTCAGACTCTGTGGTTTGAGAAAGGCCAGCCTCTGGTGTGGAAAAGGA9320.12023339727720377No Hit
TGTCTATACTACCCAAAATCTACATATTTGATGTAATACCTATCAAAATA9120.11765328145580455No Hit
GGCTGGAGCTGACCACGTGGTGGAAAGAGCCAGTTTGGGAACTAGGTGTC8590.11081597452909661No Hit
CCTTGGAGAAGGACCTGCATGGTGGGGGTGATACTGGGAAGGGAGACACG8450.10900989345411716No Hit
CCCCTAGTGGTCTCTAACGACTGGACGCAGAGCAGCCGGAGCAGAGCTGG7960.10268860969168905No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGGTTA259.422516E-486.914879
GCCGACC400.006044446554.388466145
GACCGCG1152.5047484E-950.385447
CTGTCGA1351.9281288E-1048.2860456
ACTGTCG1505.4205884E-1043.4546365
AGTGCGT855.3713076E-542.605338
GGACAGT3450.039.888476
TACATAC800.002046140436.214536
ACATCGA800.002046140436.214538
TTATATG6700.035.6855283
TACCCTA2252.0008883E-1135.4097677
AGCGAAA4950.035.1670381
TATCTAC2302.7284841E-1134.6511653
GTTCTAA1051.848528E-434.5390551
GTCGAGG1707.8864105E-834.0842678
CGAAAGC5500.032.9329263
TCAATAC2206.2755134E-1032.9329263
CCGAACA900.00364401232.1927724
CAATAGT900.003645163532.1906938
AAAGGGT5200.032.0338675
Produced by FastQC (version 0.11.5)