FastQCFastQC Report
Fri 2 Oct 2020
HTGTLBGXG_n02_S2_R1_G05_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTGTLBGXG_n02_S2_R1_G05_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAAACTATATGAGAACATCGGAGCAAGTGTCTGGTTTGTGGTTCTAAGTT15.555555555555555No Hit
GGGCTATCGCAACACCTGGGATGCAGATTAGAGGTTTCGTATACTTTGTT15.555555555555555No Hit
CGCCAGGAAAGAGAAAATGGAGATAACAACCCATGAACAAACAACAAGAC15.555555555555555No Hit
GAGTAGGATGCAATTTTCCTCATTGACTGTGAATGTGAGAGGATCAGGGT15.555555555555555No Hit
CAACAAGACACAAGCTTGATCAAAGATTTGAAGAAAGTGGAGCCCTGGGG15.555555555555555No Hit
CTTTAAAGGCATAATTTCTTTAAATGAGTCCATGAGGGTGTGCCTTAATA15.555555555555555No Hit
AATCAAACCACCGTGGTGAACGCATGGGCGAAGGACCTGCAGGGTGGGGG15.555555555555555No Hit
GAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACTCGCGAGA15.555555555555555No Hit
CAAATTCCCATCAAATTCGTCTACACTCACAGAATAGCATTTTGCCATTT15.555555555555555No Hit
GACTACCAGTACCAATGAACTGGCGACAGTTGAATAGATCGCCAAAATCT15.555555555555555No Hit
GAGCAAAACAAACGATGCTGGATCAGACCGAGTGATGGTATCACCTCTGG15.555555555555555No Hit
ATTGTATCCAGAAGCTCGAAAAGAAGATTTCGTAGACACCTGCTGTCAGT15.555555555555555No Hit
CTCTTGGAAATGTGCCACAGCACACAGATTGGAGGAGTAAGGATGGTGGA15.555555555555555No Hit
GTAAAGAACGAACGTTGGTGCAAATGGTGAGTTTGGAACCATTTCTTCGC15.555555555555555No Hit
TGTCTGGCGCTGCGGCATGGGCGATTGTCGCCGTCCTGTTCACTCTTCGG15.555555555555555No Hit
GTTTGAGATATTCCCCAAGACAAGTTCATGGCCCAATCATGACTCGAACA15.555555555555555No Hit
GGAGAATTCAGAACTACCGGAGGATGAAGATGAAGAGGAGAATGATGAAG15.555555555555555No Hit
CTATAAGACGCTGCCCCTGCGCTGTGTGCGCCCCGAGCGCGAGAGCGCGC15.555555555555555No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)