FastQCFastQC Report
Fri 2 Oct 2020
HTGTLBGXG_n02_S2_R1_G05-FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTGTLBGXG_n02_S2_R1_G05-FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences846922
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATACA48490.5725438706279917No Hit
TTTTTAAACAATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCG47680.562979825769079No Hit
AATATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCA39610.46769360106361624No Hit
AGTAGAAACAAGGCATTTTTTCATGAAGGACAAGTTAAATTCATTATTTT33300.393188510866408No Hit
GAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACTCGCGAGATA32560.38445098840271No Hit
GTAGAAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATCC29690.35056357019890855No Hit
CCTATATGAACTGCTAGGGAAAAATTTCTCGAATAGATTGCAGCACTTCT27760.3277751670165612No Hit
AGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACAATGCTTCAATCC27530.3250594505751415No Hit
GGTCAAATATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATG27140.3204545400875169No Hit
TTTTTGGACAGTATGGATAGCAAATAGTAGCATTGCCACAACTACTTCAG25400.2999095548350379No Hit
GGATACACCATGGACACAGTAAACAGAACACACCAATACTCAGAAAAGGG22830.2695643754678707No Hit
CACTAAGACCACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAG22570.26649443514278764No Hit
GTATGGATAGCAAATAGTAGCATTGCCACAACTACTTCAGTGCATGTGTG22090.26082685300417274No Hit
AAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATCCGAAT21830.2577569126790897No Hit
TTCTTGATCCGTCCAGACTCGAAGTCGACCCTGGCATCAATCCGGGCCCT20410.24099031551902064No Hit
ATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCC19770.23343353933420077No Hit
ATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCT19530.23059974826489335No Hit
GTTAAATTCATTATTTTTGCCGTCTGAGTTCTTCAATGGTGGAACAGATC19440.22953707661390305No Hit
ATCTAATGTCGCAGTCCCGCACTCGCGAGATACTCACTAAGACCACTGTG18910.2232791213358491No Hit
CATATAGACTGTTGAATACAGATTTTGCCAGTAAGGTCCTGCACACTTTC18390.21713924068568297No Hit
AATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAAGT18190.21477774812792677No Hit
CTTCAATGGTGGAACAGATCTTCATGATCTCAGAGAACTCTTCTTTCTTG14780.1745143000181835No Hit
CTTCTTTCTTGATCCGTCCAGACTCGAAGTCGACCCTGGCATCAATCCGG14760.17427815076240788No Hit
GTCCAGACTCGAAGTCGACCCTGGCATCAATCCGGGCCCTAGACACCATG14110.16660329994970022No Hit
AGTAGAAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATC13820.16317913574095372No Hit
ATATAGTGGTATTCCCTCCCCCTCGTGACTCGGGTCTTCAATACTTAATT12780.15089937444062146No Hit
CAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCA12660.14948247890596772No Hit
ATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATACACCATG12650.14936440427807993No Hit
AAACAAGGCATTTTTTCATGAAGGACAAGTTAAATTCATTATTTTTGCCG12530.1479475087434262No Hit
GATCTTCATGATCTCAGAGAACTCTTCTTTCTTGATCCGTCCAGACTCGA12510.14771135948765057No Hit
CCATTTGAATGGATGTCAATCCGACTCTACTTTTCCTAAAAATTCCAGCG11960.141217254953821No Hit
CCATACAGCCATGGAACAGGAACAGGATACACCATGGACACAGTAAACAG11840.1398003594191673No Hit
CATTTGGAAGTTTGTTTCATGTATTCGGATTTCCATTTCATCGACGAACG11830.13968228479127948No Hit
GTTCATTGATGCTTAATGCTGGGCCATATCTCTTGTCTTCTTTGCCCAAA11500.13578582207098175No Hit
CCACTATATGATGCAATCAAATGCATGAAGACATTCTTTGGCTGGAAAGA11250.1328339563737865No Hit
CAGTAAACAGAACACACCAATACTCAGAAAAGGGAAAGTGGACGACAAAC10530.12433258316586415No Hit
CCATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGAGAAGAACC10450.12338798614276167No Hit
ACACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAG10370.12244338911965919No Hit
CTCCTATATGAACTGCTAGGGAAAAATTTCTCGAATAGATTGCAGCACTT10350.12220723986388357No Hit
TTATAGGTGCCATGCTCAGGATGTTTCTGAACCACTCGGGTTGATTTCTG10280.12138071746866891No Hit
CTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAA10170.12008189656190298No Hit
GTTGAATACAGATTTTGCCAGTAAGGTCCTGCACACTTTCCCAATAGAGC10070.11890115028302488No Hit
TAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACTCGCGAGATACTC10050.11866500102724925No Hit
ATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACT9820.11594928458582962No Hit
GAATAAGAGAGGCTATCTAATAAGAGCACTGACATTAAATACGATGACCA9810.11583120995794181No Hit
GTCTATATGCGTCTCCACAACTTGAGGGGTTTTCGGCTGAATCTAGAAAA9640.11382394128384905No Hit
CTATTAGAAGAGGCCTTATTTTCTCAATTTTCTTCTTTGTTGATTCATTG9550.11276126963285875No Hit
CACTACGTCCCCTTGCCCAATTAGCACATTAGCCTTCTCTCCTTTTGCAA9480.11193474723764409No Hit
GCTTAATGCTGGGCCATATCTCTTGTCTTCTTTGCCCAAAATGAGAAATC9100.1074479113779073No Hit
GTATATTGTCTTGGTGTATTTCTTTTGTCCAAGATTCAGTATCGAGACTC9100.1074479113779073No Hit
GCTTCATATAGCCCCCCAAGATCGAAGGTTCCAGGTTCCAGGTTGTCCCT9100.1074479113779073No Hit
ATGGAAGACTTTGTGCGACAATGCTTCAATCCAATGATCGTCGAGCTTGC8960.10579486658747796No Hit
GGACAAGTTAAATTCATTATTTTTGCCGTCTGAGTTCTTCAATGGTGGAA8870.10473219493648765No Hit
GTCCCGCACTCGCGAGATACTCACTAAGACCACTGTGGACCATATGGCCA8820.10414182179704859No Hit
CTTCTAATAGATGGCACAGCATCACTGAGTCCTGGGATGATGATGGGCAT8780.10366952328549736No Hit
CTACGTCCCCTTGCCCAATTAGCACATTAGCCTTCTCTCCTTTTGCAAGA8740.10319722477394612No Hit
AACTTATACAATATACGGAATCTTCACATTCCTGAAGTCTGCTTAAAATG8690.10260685163450706No Hit
GTAGAAACAAGGCATTTTTTCATGAAGGACAAGTTAAATTCATTATTTTT8540.10083573221618992No Hit
GTATATGTAACACAACAGGGGTAGAGAAGCCTAAATTTCTTCCTGATTTG8510.10048150833252649No Hit
CACCAATACTCAGAAAAGGGAAAGTGGACGACAAACACAGAGACTGGTGC8500.10036343370463868No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTAGAA7900.0112.090211
CAGGTAC3850.0103.5001143
GCAGGTA4000.0103.314612
GGTACTG3900.0102.124925
GAGCGAA301.5902537E-596.777341
AGCGAAA1400.088.136511
CGAAAGC1600.086.034473
GCGAAAG1650.079.092522
AGGTACT5800.068.674264
CCGCACA554.0534505E-665.929924145
GAAAGCA2250.064.373454
GTAGAAA13800.064.095512
AAATATA7650.063.4230355
TACTCTA702.3538814E-762.067077
TATATTC10950.059.548013
GGTCAAA8800.059.3860971
ATATTCA11550.056.431274
GATCTTA1450.055.0629731
ACTGATC7450.054.4333958
CCCGTTG400.006043004354.39219145
Produced by FastQC (version 0.11.5)