FastQCFastQC Report
Fri 2 Oct 2020
HTGTLBGXG_n02_S2_R1_G02-FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTGTLBGXG_n02_S2_R1_G02-FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences855766
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGATGTACTGTGCACGCGGGGAGCGGTGGCGATCAGTTACGGGGAACA17400.20332661031169735No Hit
CTTTATATACTGACAGGCCCTCTGTAGATGTACTGTGCACGCGGGGAGCG13390.15646800644101308No Hit
CCTATATGAACTGCTAGGGAAAAATTTCTCGAATAGATTGCAGCACTTCT11410.13333084044002683No Hit
CTATTAGAAGAGGCCTTATTTTCTCAATTTTCTTCTTTGTTGATTCATTG10490.12258023805573018No Hit
GTTCACAGCAGTAGGTAAAGAGTTCAACCACCTGGAAAAAAGAATAGAGA9880.11545212125744653No Hit
AAATAAGACAGGGACATTTGAATTCACAAGCTTTTTTTATCGCTATGGAT9800.1145172862675077No Hit
GCTAATAGAGTGAGCTTCACAGGGCATTCCATCCCCTTGTTGAGTCTCAA9530.11136221817646412No Hit
GTATAAGTTTGGTCCTCCATCTGATACTAATAGCCCTACCTTTGATTGGG9470.11066109193401No Hit
CTGTAGATGTACTGTGCACGCGGGGAGCGGTGGCGATCAGTTACGGGGAA9080.10610377135805817No Hit
TCCTTAGACAGAATCCAACTGAGGAACAAGCCGTAGACATATGCAAGGCA8980.10493522762063462No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA1400.0103.6126861
CAATTCG2350.064.722149
AGGTACT1150.062.991184
TTATACT2150.057.2780274
CCTTATA2350.055.528042
AATCGGC400.00604665654.38393145
TCGTAAT604.9349735E-448.352591
GATAGCA1751.8189894E-1245.5256426
TGCCGGA1151.4234683E-744.0860989
GTGGTAT2600.041.7848976
CCCTCGG907.47429E-540.28439145
GGTACTG2005.456968E-1239.8395925
TAGTAAC951.0263556E-438.1730961
GTATTAA3050.038.0479351
GAGTGCA2109.094947E-1237.9380349
ATAGTGG3100.037.3948673
CCACTAT3800.036.264441
GCAGGTA2401.8189894E-1236.247472
GGAGGGC1458.765128E-734.9648328
GGACTCG1051.8644739E-434.489126
Produced by FastQC (version 0.11.5)