FastQCFastQC Report
Fri 2 Oct 2020
HTGTLBGXG_n02_S2_R1_F12-FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTGTLBGXG_n02_S2_R1_F12-FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences281265
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTATGTACAGTGCACATGTATCTCTCATAAACTCACTATATAACTCACT19800.7039624553357154No Hit
GAATTAATACAATCATGTTACATAGAGATGCTATGATGCTAAAAATGGGA13360.474996889054806No Hit
CATTTACAAACTGTACTTGTGAGAGCCTTCATTGACTGTTAAGTTGTTAC13210.4696638401507475No Hit
GTACTTGTGAGAGCCTTCATTGACTGTTAAGTTGTTACACTAATTACATT10980.39037917977707853No Hit
AGTCTATAGTGGCTATATGTAATGGTAGAATGAGGATAGTGAAGTATAGA10730.38149076493698114No Hit
ACTGTAGACTATCTGCAGAAGAGTAATTACAGGACAAGGTAACATTCAGT10140.36051410591435123No Hit
TTTACAAACTGTACTTGTGAGAGCCTTCATTGACTGTTAAGTTGTTACAC10010.3558921301975006No Hit
CCTCTTAGTTTTACTGTAATGTTGGCTAGGAACTGCTTCCTGCTGGAGCA8890.31607203171386417No Hit
GCTCTATACTGTACTCTAGTAGCCACCTCCACAATGTTGCTGTTTCAACT8070.2869180310383446No Hit
GATCTGGGATACTGAATGTACCACTGTAGACTATCTGCAGAAGAGTAATT7700.2737631770750005No Hit
CACACACACACACACACACACACACACACACACACACACACACACACACA7540.2680745915773381No Hit
GTGTGAGACTAAGAGGGGTAGTAACACACACCTCTTAGTTTTACTGTAAT7160.25456420102039No Hit
TTACAGGACAAGGTAACATTCAGTCCTTCCTCTGTAAAGACTTCAGTTCT7120.25314205464597445No Hit
AATTACATTAATGTCTCACTTGTGTGCAAGTTTAGACTGAGAGCATGTGT7100.25243098145876663No Hit
ACATAGAGATGCTATGATGCTAAAAATGGGACAGGAAACTTAAGCACAGT7020.24958668870993547No Hit
CTACTATAGTCTATAGTGGCTATATGTAATGGTAGAATGAGGATAGTGAA6880.24460917639948093No Hit
TACAAAGACACACCACAAACATCTCTTACTATGTACAGTGCACATGTATC6850.24354256661866924No Hit
ATGTACCACTGTAGACTATCTGCAGAAGAGTAATTACAGGACAAGGTAAC6710.23856505430821465No Hit
TAGTAGGACAGCACAGAGTAACGTAGGATAGCATAGAGTATAGTAGGATA6700.23820951771461074No Hit
GTATGGATTATTCATCACGGTGGCTCCTGTTCTTTACGTCCAGTAGCCCA6560.23323200540415623No Hit
GCATAGAGTATAGTAGGACAGCACAGAGTAACGTAGGATAGCATAGAGTA6540.23252093221694842No Hit
CACACACACGGAGAGAGATTAGGTCATAAAAGTAACAGAACCAAAGGGTT6340.2254102003448705No Hit
ACTCTGATCTGAGCAAAATGATCACTCGAGTCAGTCCTGTAAATCTTTTT6240.22185483440883155No Hit
GAATGTACCACTGTAGACTATCTGCAGAAGAGTAATTACAGGACAAGGTA6030.2143885659431497No Hit
GTTAAGTTGTTACACTAATTACATTAATGTCTCACTTGTGTGCAAGTTTA5820.20692229747746788No Hit
GTTACATAGAGATGCTATGATGCTAAAAATGGGACAGGAAACTTAAGCAC5780.20550015110305228No Hit
GTATGTAGCTGCCTGATTACTCAAATCCTCTGTGCAACCCCTATCACACA5760.2047890779158445No Hit
CTACCACACCCAATCAAAAACAAACACAGAAACAAATACATACAAAGACA5680.2019447851670133No Hit
ATACTGTACTCTAGTAGCCACCTCCACAATGTTGCTGTTTCAACTCATCT5360.19056761417168863No Hit
CTATATAACTCACTAGGGACACATGCTCTCAGTCTAAACTTGCACACAAG5340.18985654098448082No Hit
CACGGAGAGAGATTAGGTCATAAAAGTAACAGAACCAAAGGGTTGCATAA5320.18914546779727304No Hit
CTGTTAAGTTGTTACACTAATTACATTAATGTCTCACTTGTGTGCAAGTT5290.18807885801646135No Hit
CACACACACACACGGAGAGAGATTAGGTCATAAAAGTAACAGAACCAAAG5250.18665671164204575No Hit
CTATACTGTACTCTAGTAGCCACCTCCACAATGTTGCTGTTTCAACTCAT5020.17847936998915614No Hit
CACACACACACACACGGAGAGAGATTAGGTCATAAAAGTAACAGAACCAA4980.17705722361474055No Hit
CACACACACACACCCCTTCCCATCTACCACACCCAATCAAAAACAAACAC4930.17527954064672108No Hit
GTGCACATGTATCTCTCATAAACTCACTATATAACTCACTAGGGACACAT4910.17456846745951327No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGT4890.17385739427230548No Hit
GTACAGTGCACATGTATCTCTCATAAACTCACTATATAACTCACTAGGGA4830.1717241747106821No Hit
GTATAGTAGGACAGCACAGAGTAACGTAGGATAGCATAGAGTATAGTAGG4820.1713686381170782No Hit
GTATAGACCATAAAGCACATTTTAGCATGTACGTTATTTTTTACAAGGAG4710.16745773558743535No Hit
ATCTGCAGAAGAGTAATTACAGGACAAGGTAACATTCAGTCCTTCCTCTG4680.16639112580662366No Hit
CATATATACATGCATACATACACACACACACACACACACACACACACACA4670.16603558921301975No Hit
GGTAAGAGATATCATTCATCAGGTACATTCAATTTGTGTTGTTGATGAAC4660.16568005261941585No Hit
GTGTGTGTGTGTGTGTATTGGTTTCACTGAGTGTGAGACTAAGAGGGGTA4630.16461344283860416No Hit
GAGTATAGTAGGACAGCACAGAGTAACGTAGGATAGCATAGAGTATAGTA4610.16390236965139637No Hit
CACACACACACGGAGAGAGATTAGGTCATAAAAGTAACAGAACCAAAGGG4590.1631912964641886No Hit
GTGTGTGTGTGTGTGTGTATTGGTTTCACTGAGTGTGAGACTAAGAGGGG4530.16105807690256518No Hit
ACTATAGTCTATAGTGGCTATATGTAATGGTAGAATGAGGATAGTGAAGT4530.16105807690256518No Hit
CACACACACACACACACGGAGAGAGATTAGGTCATAAAAGTAACAGAACC4480.1592803939345457No Hit
CTGTACTTGTGAGAGCCTTCATTGACTGTTAAGTTGTTACACTAATTACA4460.15856932074733793No Hit
GTACAGTAAGCACTCCTTGTAAAAAATAACGTACATGCTAAAATGTGCTT4420.15714717437292233No Hit
TTCATTGACTGTTAAGTTGTTACACTAATTACATTAATGTCTCACTTGTG4420.15714717437292233No Hit
GGGATACTGAATGTACCACTGTAGACTATCTGCAGAAGAGTAATTACAGG4400.15643610118571455No Hit
GGATAGCATAGAGTATAGTAGGACAGCACAGAGTAACGTAGGATAGCATA4350.15465841821769505No Hit
CATAGATACTAATTATTCAGTAAAACACCATTCTCTTCTTCAAATCGTTT4330.15394734503048726No Hit
GTCTACAAGGCACAGGGCTGCACTCTGCTGTGATATACTGCTGCTCTATG4300.15288073524967558No Hit
GTGATATACTGCTGCTCTATGCTATCCTACTGTACTCTATGCTATCCTGC4280.1521696620624678No Hit
CACACACCCCTTCCCATCTACCACACCCAATCAAAAACAAACACAGAAAC4270.1518141254688639No Hit
GAGAGAGATTAGGTCATAAAAGTAACAGAACCAAAGGGTTGCATAAATGC4240.1507475156880522No Hit
CACACGGAGAGAGATTAGGTCATAAAAGTAACAGAACCAAAGGGTTGCAT4240.1507475156880522No Hit
GTGTGTGTGTGTGTGTGTGTATTGGTTTCACTGAGTGTGAGACTAAGAGG4220.15003644250084439No Hit
GTGTATTGGTTTCACTGAGTGTGAGACTAAGAGGGGTAGTAACACACACC4210.14968090590724048No Hit
CACACACACACACACACACGGAGAGAGATTAGGTCATAAAAGTAACAGAA4190.14896983272003272No Hit
GTGTGTGTGTGTGTATTGGTTTCACTGAGTGTGAGACTAAGAGGGGTAGT4100.14577000337759763No Hit
GTCTTAATACTACTATAGTCTATAGTGGCTATATGTAATGGTAGAATGAG4090.14541446678399375No Hit
TTGTTACACTAATTACATTAATGTCTCACTTGTGTGCAAGTTTAGACTGA4080.14505893019038985No Hit
GTAAAACACCATTCTCTTCTTCAAATCGTTTCCATTTCTCTGGTTTAAAG4070.14470339359678597No Hit
CTCATAAACTCACTATATAACTCACTAGGGACACATGCTCTCAGTCTAAA4070.14470339359678597No Hit
ACATTAATGTCTCACTTGTGTGCAAGTTTAGACTGAGAGCATGTGTCCCT4050.14399232040957816No Hit
CTGTAGACTATCTGCAGAAGAGTAATTACAGGACAAGGTAACATTCAGTC3950.14043695447353918No Hit
AGTTTAGACTGAGAGCATGTGTCCCTAGTGAGTTATATAGTGAGTTTATG3950.14043695447353918No Hit
GGCTATATGTAATGGTAGAATGAGGATAGTGAAGTATAGACCATAAAGCA3940.1400814178799353No Hit
CACACACGGAGAGAGATTAGGTCATAAAAGTAACAGAACCAAAGGGTTGC3920.1393703446927275No Hit
CTCTTACTATGTACAGTGCACATGTATCTCTCATAAACTCACTATATAAC3910.1390148080991236No Hit
GAACTGGGGAGCTGATCTGGGATACTGAATGTACCACTGTAGACTATCTG3880.13794819831831193No Hit
CTCATACACTGAAGCATGGACGTATAAGAAGATGCGTCTGTCACTCAACT3870.13759266172470802No Hit
AGGTAACATTCAGTCCTTCCTCTGTAAAGACTTCAGTTCTGTCTGGAGTG3810.13545944216308464No Hit
GGGCTGCACTCTGCTGTGATATACTGCTGCTCTATGCTATCCTACTGTAC3790.13474836897587683No Hit
GTGTTAGAGAGAGCATGTGTCGGAGATAATTAAAATATGTGCTCGTTAGA3760.13368175919506517No Hit
ATCAAAAACAAACACAGAAACAAATACATACAAAGACACACCACAAACAT3740.13297068600785736No Hit
CACACACACACACACACACACGGAGAGAGATTAGGTCATAAAAGTAACAG3710.13190407622704567No Hit
GTTATATAGTGAGTTTATGAGAGATACATGTGCACTGTACATAGTAAGAG3690.13119300303983786No Hit
GTACTCTAGTAGCCACCTCCACAATGTTGCTGTTTCAACTCATCTCATGT3640.12941532007181838No Hit
GTATATATGTCTGCATGTATGTATGTGTGTGTGTGTGTGTGTATGTATGT3640.12941532007181838No Hit
CCTTTAAACCAGAGAAATGGAAACGATTTGAAGAAGAGAATGGTGTTTTA3620.1287042468846106No Hit
GCTCTATGCTATCCTACTGTACTCTATGCTATCCTGCTGTACTCTATGCT3620.1287042468846106No Hit
ATATAACTCACTAGGGACACATGCTCTCAGTCTAAACTTGCACACAAGTG3590.1276376371037989No Hit
ATGTAATGGTAGAATGAGGATAGTGAAGTATAGACCATAAAGCACATTTT3580.127282100510195No Hit
CACTAATGGAGGTAAAGACACAGACACAATCAGGCCCTTTGTGGATGGAC3570.1269265639165911No Hit
GGTACATTCAATTTGTGTTGTTGATGAACATAGATACTAATTATTCAGTA3570.1269265639165911No Hit
CATACATACATGCAGACATATATACATGCATACATACACACACACACACA3500.12443780776136383No Hit
GTGTGTGTGTGTGTGTGTGTGTATTGGTTTCACTGAGTGTGAGACTAAGA3440.12230458819974047No Hit
AAGTAGGAGTGACTCACAGTGAGCTCTATACTGTACTCTAGTAGCCACCT3440.12230458819974047No Hit
GTGAAGTATAGACCATAAAGCACATTTTAGCATGTACGTTATTTTTTACA3410.12123797841892878No Hit
GACTAAGAGGGGTAGTAACACACACCTCTTAGTTTTACTGTAATGTTGGC3390.12052690523172097No Hit
CCCCTAAGGCTCCCAATCTGCTCCAGCAGGAAGCAGTTCCTAGCCAACAT3360.11946029545090928No Hit
ATGCTATCCTACTGTACTCTATGCTATCCTGCTGTACTCTATGCTATCCT3340.1187492222637015No Hit
CTACAGAGGGAGGGAGAGAAAGAGACAAAGAGAAAGAGAGAGAGTGAGAG3330.1183936856700976No Hit
GTAGTAACACACACCTCTTAGTTTTACTGTAATGTTGGCTAGGAACTGCT3320.11803814907649371No Hit
GTTGTTACACTAATTACATTAATGTCTCACTTGTGTGCAAGTTTAGACTG3310.1176826124828898No Hit
ATGTTACATAGAGATGCTATGATGCTAAAAATGGGACAGGAAACTTAAGC3310.1176826124828898No Hit
AACAAATACATACAAAGACACACCACAAACATCTCTTACTATGTACAGTG3280.11661600270207811No Hit
CTCTAGTGCCATCCAAAACACTCATCAACTCTCCTTCTCTGCTCTCAACT3230.11483831973405864No Hit
CAGTATGGATTATTCATCACGGTGGCTCCTGTTCTTTACGTCCAGTAGCC3230.11483831973405864No Hit
GTTCTAAACTGCACCCTTGTGTCCTTTCAGAGACTACGCCTGTCTGTGGG3200.11377170995324694No Hit
CATCACTGACAGTGTTTGGTATGTACTTTTTATACATCCACTGAATTAAT3180.11306063676603914No Hit
CAATTAAGTAGCGGAAATTAAGCTCCTTTAATTAAGTCGTCTTTCCCCTA3150.11199402698522745No Hit
GACACAATCAGGCCCTTTGTGGATGGACATAACTCTGATCTGAGCAAAAT3120.11092741720441578No Hit
CTTTTTATACATCCACTGAATTAATACAATCATGTTACATAGAGATGCTA3120.11092741720441578No Hit
CCACAAACATCTCTTACTATGTACAGTGCACATGTATCTCTCATAAACTC3110.11057188061081187No Hit
GTGTTTGGTATGTACTTTTTATACATCCACTGAATTAATACAATCATGTT3080.10950527083000018No Hit
ATCCTACTGTACTCTATGCTATCCTGCTGTACTCTATGCTATCCTACGTT3070.10914973423639628No Hit
ATTTAGAAACTGACCAATTCTTTAAAATGTATGTAGCTGCCTGATTACTC3070.10914973423639628No Hit
GAGGGAGAGAAGTCAAATCATGCAAACATTTACAAACTGTACTTGTGAGA3060.10879419764279238No Hit
TCTCACTTGTGTGCAAGTTTAGACTGAGAGCATGTGTCCCTAGTGAGTTA3060.10879419764279238No Hit
GTGCAACCCCTATCACACACAGACACAAGTAAACTCATACACTGAAGCAT3040.10808312445558459No Hit
GTAACACACACCTCTTAGTTTTACTGTAATGTTGGCTAGGAACTGCTTCC3020.1073720512683768No Hit
GTCCTTCACTAGCCGTTACCACCAGACTGGTATTTGCATGGAAGGCTTTG3020.1073720512683768No Hit
CACACACACACACACACACACACGGAGAGAGATTAGGTCATAAAAGTAAC3010.10701651467477291No Hit
CCTTCATTGACTGTTAAGTTGTTACACTAATTACATTAATGTCTCACTTG2970.10559436830035732No Hit
ATACTGAATGTACCACTGTAGACTATCTGCAGAAGAGTAATTACAGGACA2970.10559436830035732No Hit
CTAGTAGCCACCTCCACAATGTTGCTGTTTCAACTCATCTCATGTTGGGC2940.10452775851954561No Hit
CATCTCTTACTATGTACAGTGCACATGTATCTCTCATAAACTCACTATAT2930.10417222192594172No Hit
CACACACACACCCCTTCCCATCTACCACACCCAATCAAAAACAAACACAG2900.10310561214513005No Hit
ATATACTGCTGCTCTATGCTATCCTACTGTACTCTATGCTATCCTGCTGT2900.10310561214513005No Hit
CACATACATACATGCAGACATATATACATGCATACATACACACACACACA2890.10275007555152614No Hit
GTATGGGTTCACACATCCCCTCTCCTCCACTCTGGAAGGAGCTGTTACGT2890.10275007555152614No Hit
GTGTGTGTGTGTGTGTGTGTGTGTATTGGTTTCACTGAGTGTGAGACTAA2880.10239453895792225No Hit
ATCACACACAGACACAAGTAAACTCATACACTGAAGCATGGACGTATAAG2880.10239453895792225No Hit
GGATTATTCATCACGGTGGCTCCTGTTCTTTACGTCCAGTAGCCCACAGC2880.10239453895792225No Hit
GTGTATTACCTTTCACCTTCACCTTTGACCTCACACACACACACACATGC2880.10239453895792225No Hit
CACACACACACACACCCCTTCCCATCTACCACACCCAATCAAAAACAAAC2880.10239453895792225No Hit
ATATGTAATGGTAGAATGAGGATAGTGAAGTATAGACCATAAAGCACATT2870.10203900236431834No Hit
GTGAAGAAGATTAAAGTAGGAGTGACTCACAGTGAGCTCTATACTGTACT2860.10168346577071445No Hit
CCCCTTCCCATCTACCACACCCAATCAAAAACAAACACAGAAACAAATAC2830.10061685598990275No Hit
CACACACACACACACACACACACACGGAGAGAGATTAGGTCATAAAAGTA2820.10026131939629886No Hit
GCATAGAGTATAGTAGGATAGCATAGAGTATAGTAGGACAGCACAGAGTA2820.10026131939629886No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATTTT100.0070845284145.02492145
AGCGAAA2650.0117.8294751
GCAGGCC406.138598E-790.5438848
TGACGGT353.448254E-582.782986
GCGAAAG3800.082.082792
CGAAACC651.5625119E-978.048673
CGAAAGC4200.075.92493
GACGTAC406.602411E-572.615841
CTACAAG903.6379788E-1272.473763
ACGGTGT406.684457E-572.4351048
TCTTAAT1100.065.943892
CTGGGAT2750.065.861824
TCTACAA1009.094947E-1265.284452
GATCTGG2900.065.103861
TGGGATA2800.064.674215
AATGACG451.19661396E-464.398214
GACGGTG451.1976676E-464.3867657
ATGACGG451.1976676E-464.3867655
ATCGAAA807.95626E-963.538861
TCGAAAC808.025381E-963.4709932
Produced by FastQC (version 0.11.5)