FastQCFastQC Report
Fri 2 Oct 2020
HTGTLBGXG_n02_S2_R1_F06-FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTGTLBGXG_n02_S2_R1_F06-FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences697906
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTAAACAATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCG68940.9878121122328795No Hit
GAATGAAGTGGATGATGGCAATGAGATACCCAATTACAGCAGACAAGAGA59660.8548429158081461No Hit
AATATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCA56590.810854183801257No Hit
CCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATACA56340.8072720395010216No Hit
GAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACTCGCGAGATA47950.6870552767851258No Hit
AGTAGAAACAAGGCATTTTTTCATGAAGGACAAGTTAAATTCATTATTTT43610.6248692517330414No Hit
CACTAAGACCACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAG41460.5940628107510181No Hit
GTAGAAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATCC37610.5388977885273949No Hit
GGTCAAATATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATG35990.5156854934618702No Hit
GCTTAATGCTGGGCCTCTTGTCTTCTTTGCCCAAAATGAGAAATCCTCTC35530.5090943479494373No Hit
GGATACACCATGGACACAGTAAACAGAACACACCAATACTCAGAAAAGGG31840.45622189807796465No Hit
ATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCT30360.4350156038205718No Hit
AAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATCCGAAT29820.42727817213206365No Hit
GTTCATTGATGCTTAATGCTGGGCCTCTTGTCTTCTTTGCCCAAAATGAG28970.4150988815112637No Hit
AATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAAGT28570.40936745063088725No Hit
ATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCC28520.40865102177084023No Hit
ATCTAATGTCGCAGTCCCGCACTCGCGAGATACTCACTAAGACCACTGTG28060.4020598762584072No Hit
GAATAATGGACATGATTCCAGAGACAACAAGGCAACCAAACGACTTACAG27730.3973314457820967No Hit
CCATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGAGAAGAACC25660.36767129097614865No Hit
TTCTTGATCCGTCCAGACTCGAAGTCGACCCTGGCATCAATCCGGGCCCT24900.3567815723034334No Hit
GTTAAATTCATTATTTTTGCCGTCTGAGTTCTTCAATGGTGGAACAGATC24760.35477557149530164No Hit
CACTACGTCCCCTTGCCCAATTAGCACATTAGCCTTCTCTCCTTTTGCAA22130.31709141345682657No Hit
CTACGTCCCCTTGCCCAATTAGCACATTAGCCTTCTCTCCTTTTGCAAGA21720.3112166968044407No Hit
CCTATATGAACTGCTAGGGAAAAATTTCTCGAATAGATTGCAGCACTTCT21070.301903121623829No Hit
AGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACAATGCTTCAATCC20990.3007568354477537No Hit
CTTCAATGGTGGAACAGATCTTCATGATCTCAGAGAACTCTTCTTTCTTG20230.28986711677503846No Hit
ACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGA20090.2878611159669067No Hit
TTTTTGGACAGTATGGATAGCAAATAGTAGCATTGCCACAACTACTTCAG18270.26178310546119393No Hit
CAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCA17810.255191959948761No Hit
AGTAGAAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATC17800.2550486741767516No Hit
CTGTGGACCATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGAG17180.24616495631216812No Hit
GAATGAATGAGATACCCAATTACAGCAGACAAGAGAATAATGGACATGAT16940.24272609778394225No Hit
CCATTTGAATGGATGTCAATCCGACTCTACTTTTCCTAAAAATTCCAGCG16900.24215295469590462No Hit
GAATGAATGAGATACCCAATTACAGCAGACAAGAGAATAAGGCAACCAAA16720.23957381079973522No Hit
AATTAGCACATTAGCCTTCTCTCCTTTTGCAAGATTGCTCAGTTCATTGA16650.23857081039566932No Hit
AAACAAGGCATTTTTTCATGAAGGACAAGTTAAATTCATTATTTTTGCCG16360.23441552300739643No Hit
ACACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAG16070.23026023561912348No Hit
CTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAA15770.22596166245884117No Hit
TAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACTCGCGAGATACTC15510.22223623238659648No Hit
CCATACAGCCATGGAACAGGAACAGGATACACCATGGACACAGTAAACAG15440.2212332319825306No Hit
CATTAGCCTTCTCTCCTTTTGCAAGATTGCTCAGTTCATTGATGCTTAAT15350.2199436600344459No Hit
CATCAGGAAGGCAAGAGAAGAACCCCGCACTCAGAATGAAGTGGATGATG15330.21965708849042706No Hit
GTCCAGACTCGAAGTCGACCCTGGCATCAATCCGGGCCCTAGACACCATG15280.21894065963038004No Hit
ATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATACACCATG14980.2146420864700977No Hit
TTACCAACACTACGTCCCCTTGCCCAATTAGCACATTAGCCTTCTCTCCT14970.2144988006980883No Hit
CTTCTTTCTTGATCCGTCCAGACTCGAAGTCGACCCTGGCATCAATCCGG14730.21105994216986243No Hit
GTATGGATAGCAAATAGTAGCATTGCCACAACTACTTCAGTGCATGTGTG14600.20919722713374006No Hit
GTCCCGCACTCGCGAGATACTCACTAAGACCACTGTGGACCATATGGCCA14200.2034657962533636No Hit
ATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACT14160.202892653165326No Hit
CTAAGACCACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAGGA14140.20260608162130717No Hit
TCTCAGGACAGCAGACTCCACCCCAGATGTGCCTTCATCTGGATCTTCAG13800.19773436537298716No Hit
CCCCAGATGTGCCTTCATCTGGATCTTCAGTCAATGCACCTGCATCCTTT13250.1898536479124696No Hit
TCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAAGTATGCTAGAGTCC13050.18698793247228135No Hit
GATCTAATGTCGCAGTCCCGCACTCGCGAGATACTCACTAAGACCACTGT12740.1825460735399896No Hit
ATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAAGTA12640.18111321581989553No Hit
GACCACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAGGAAGGC12470.17867735769573553No Hit
ATCAAAAAGTACACATCAGGAAGGCAAGAGAAGAACCCCGCACTCAGAAT12430.1781042146076979No Hit
AATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCAC12190.17466535607947203No Hit
GTACACATCAGGAAGGCAAGAGAAGAACCCCGCACTCAGAATGAAGTGGA12180.1745220703074626No Hit
ATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTC12130.17380564144741556No Hit
ATACTCACTAAGACCACTGTGGACCATATGGCCATAATCAAAAAGTACAC12080.17308921258736848No Hit
GGATCTTCAGTCAATGCACCTGCATCCTTTCCAAGAACTGTAAGTCGTTT11940.17108321177923674No Hit
CTTATCTCTTGTCTTCTTTGCCCAAAATGAGAAATCCTCTCAGGACAGCA11850.16979363983115203No Hit
GGACAAGTTAAATTCATTATTTTTGCCGTCTGAGTTCTTCAATGGTGGAA11820.16936378251512382No Hit
CATATAGACTGTTGAATACAGATTTTGCCAGTAAGGTCCTGCACACTTTC11800.16907721097110498No Hit
GATCTTCATGATCTCAGAGAACTCTTCTTTCTTGATCCGTCCAGACTCGA11710.1677876390230203No Hit
GTAGAAACAAGGCATTTTTTCATGAAGGACAAGTTAAATTCATTATTTTT11550.16549506667086972No Hit
CCCTTGCCCAATTAGCACATTAGCCTTCTCTCCTTTTGCAAGATTGCTCA11260.1613397792825968No Hit
TGCTTAATGCTGGGCCTCTTGTCTTCTTTGCCCAAAATGAGAAATCCTCT10670.15288591873404156No Hit
CTTAATGCTGGGCCTCTTGTCTTCTTTGCCCAAAATGAGAAATCCTCTCA10080.1444320581854863No Hit
AGTTAAATTCATTATTTTTGCCGTCTGAGTTCTTCAATGGTGGAACAGAT10030.14371562932543924No Hit
GAGAAGAACCCCGCACTCAGAATGAAGTGGATGATGGCAATGAGATACCC10010.14342905778142043No Hit
GAACTGAGAGATCTAATGTCGCAGTCCCGCACTCGCGAGATACTCACTAA9780.14013348502520398No Hit
TACCAACACTACGTCCCCTTGCCCAATTAGCACATTAGCCTTCTCTCCTT9610.13769762690104398No Hit
CCTCCATACAGCCATGGAACAGGAACAGGATACACCATGGACACAGTAAA9590.13741105535702514No Hit
CAATTAGCACATTAGCCTTCTCTCCTTTTGCAAGATTGCTCAGTTCATTG9440.13526176877688398No Hit
ATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGAGAAGAACCCC9090.1302467667565546No Hit
GAATAAGGCAACCAAACGACTTACAGTTCTTGGAAAGGATGCAGGTGCAT9010.12910048058047932No Hit
GTGGACCATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGAGAA8880.12723776554435698No Hit
AAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCTACTTTTCCTA8860.12695119400033816No Hit
GCTTATCTCTTGTCTTCTTTGCCCAAAATGAGAAATCCTCTCAGGACAGC8640.12379890701613111No Hit
GGCAAGAGAAGAACCCCGCACTCAGAATGAAGTGGATGATGGCAATGAGA8620.1235123354721123No Hit
CCCAATTACAGCAGACAAGAGAATAATGGACATGATTCCAGAGACAACAA8470.12136304889197112No Hit
CAGTAAACAGAACACACCAATACTCAGAAAAGGGAAAGTAAGAGGAATCG8440.12093319157594289No Hit
GGCCTCCACCATGCTAGAAATTCCAACCGGTCTCCTATATGAACTGCTAG8430.12078990580393348No Hit
CTCCTATATGAACTGCTAGGGAAAAATTTCTCGAATAGATTGCAGCACTT8240.11806747613575468No Hit
CTTCTGGTACATCTGTTCATCCTCAAGAATTCCCCTTTGGCTTGTGTTGA8170.1170644757316888No Hit
GTGGAACAGATCTTCATGATCTCAGAGAACTCTTCTTTCTTGATCCGTCC8150.11677790418766996No Hit
GTTCATTGATGCTTATCTCTTGTCTTCTTTGCCCAAAATGAGAAATCCTC8120.11634804687164174No Hit
CCACATTCCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGAACA8100.11606147532762291No Hit
CTTGTCTTCTTTGCCCAAAATGAGAAATCCTCTCAGGACAGCAGACTCCA8010.11477190337953823No Hit
CTTTTGGTCGCTGTCTGGCTGTCAGTAAGTATGCTAGAGTCCCGTTTTCG7890.1130524741154253No Hit
ATCTCAGAGAACTCTTCTTTCTTGATCCGTCCAGACTCGAAGTCGACCCT7790.11161961639533118No Hit
GGACAGCAGACTCCACCCCAGATGTGCCTTCATCTGGATCTTCAGTCAAT7770.11133304485131236No Hit
CGCGAGATACTCACTAAGACCACTGTGGACCATATGGCCATAATCAAAAA7570.10846732941112414No Hit
ATGGAAGACTTTGTGCGACAATGCTTCAATCCAATGATCGTCGAGCTTGC7550.1081807578671053No Hit
ACACTACGTCCCCTTGCCCAATTAGCACATTAGCCTTCTCTCCTTTTGCA7440.1066046143750018No Hit
GTATATGTAACACAACAGGGGTAGAGAAGCCTAAATTTCTTCCTGATTTG7390.10588818551495473No Hit
GTATGCTAGAGTCCCGTTTTCGTTTCATTACCAACACTACGTCCCCTTGC7370.10560161397093593No Hit
GCCATAATCAAAAAGTACACATCAGGAAGGCAAGAGAAGAACCCCGCACT7360.1054583281989265No Hit
GAACAGATCTTCATGATCTCAGAGAACTCTTCTTTCTTGATCCGTCCAGA7320.10488518511088886No Hit
GTCTATATGCGTCTCCACAACTTGAGGGGTTTTCGGCTGAATCTAGAAAA7240.10373889893481357No Hit
GCGGAAAAGGCAATGAAAGAATATGGGGAAGATCCGAAAATCGAAACTAA7230.10359561316280416No Hit
TTGCAAGATTGCTCAGTTCATTGATGCTTAATGCTGGGCCTCTTGTCTTC7220.10345232739079474No Hit
GGACCATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGAGAAGA7210.10330904161878535No Hit
CAATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGG7200.10316575584677593No Hit
TGGTGGAACAGATCTTCATGATCTCAGAGAACTCTTCTTTCTTGATCCGT7100.1017328981266818No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGCCCG100.007084595145.03976145
TACGGAG100.0071119876144.852683
AGTAGAA8450.0116.693261
AGCGAAA1500.0111.173211
AGAGTTA203.8613463E-4108.77982145
GCAGGTA3600.096.6099852
CAGGTAC3650.095.24563
GCGAAAG1800.092.5845642
CACATCG554.1472958E-1092.29802145
CGAAAGC1750.091.050253
GGTACTG3950.087.999145
AGGGTGA259.4288966E-486.899156
GGTCAAA7650.080.5602951
GCTATAC406.6954104E-572.426343
CTAACCG300.001943317472.426333
CCGAGGT300.001944422572.4159557
AAATATA8100.071.5219355
GTAGAAA13800.071.407382
GAAAGCA2350.067.7985154
GACAGTA5400.067.051817
Produced by FastQC (version 0.11.5)