FastQCFastQC Report
Fri 2 Oct 2020
HTGTLBGXG_n02_S2_R1_F05_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTGTLBGXG_n02_S2_R1_F05_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CATATAGACAGATGAATACAGAATTTGCCAGTAAGGTCCTGCACACTTTC14.0No Hit
GGCCCATCAGGCAATCTGACGGGGCGTGGTGTCGTCCTCAAGAATGGTTC14.0No Hit
ACCAAGAAGATGGTCACGCAAAGAACAATAGGGAAGAAAAAACAAAGACT14.0No Hit
ATAAGGAGTGTGGGCCATTGATAATGCTTTAGTTATCCCGCAAGTACAAG14.0No Hit
GTCAGGTGGTTAGTAATCAGTCTTGGTGTATGGTCAATCTGTGCTGTATG14.0No Hit
CAGCGACATCTCCGTGCTTGGGGTCATGTCGGGCAGTATTCCGATCATTC14.0No Hit
CACCATAGAAGTCTTTAGATCGAATGGCCTAACAGCTAATGAGTCAGGAA14.0No Hit
GAAAAATAAAATAACCCAACAATTAAAAAGAAAAAGAATAGAAAAAGAAA14.0No Hit
TATGAGTGGACATTAAACAGAAATCAACCGGCAGCAACTGCATTGGCAAA14.0No Hit
CCCCCTCGTGACTAGGGTCTTCAATACTTAATTTCAGAGCATCAATCAGC14.0No Hit
CTTTAGCGCGAATGGTCTAACAGCTAATGAGTCAGGAAGGCTAATAGATT14.0No Hit
CCCCTGGGTGACTCTCCGATTGGCCATGTTTCCGATTTGTTTTCAAAGAA14.0No Hit
GAATGAAAAGATGTGAATGTGTGTCTTCTCAGATTTTATTTTGTTGGCTT14.0No Hit
GTAAAATAGTTCCTCCTCATGCTTGCGATATGTTCAATCGGGGCAACATC14.0No Hit
GAGAAGAACACCGCACTCAGAATGAAGTGGATGAAGGCAATGAGATACCC14.0No Hit
CACAATCGAGCCTCCAAAGCCCGTTATTAGGATGTGCACCATCCGCATCA14.0No Hit
GCACCAAACCATGAGGGAATACAAGCAGGAGTGGACAGATTCTACAGGAC14.0No Hit
CACTTCTCCTACTGGATTGTACATCTCCTCCCAGCACGTCCCTTGGGTTA14.0No Hit
ACTATGAACATATGGATTTCTCGCTCACTGACACGAGCCCGGAGCCACAC14.0No Hit
GGGGTGTGCTGCTCAATGCTGGGGCCGATGTGTGCTGGTGTTTGGGGAAG14.0No Hit
GCAAGGAACTGAGAAGTTGACAATAACTTATTCGTCATCAATGATGTGGG14.0No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG14.0No Hit
ACACAACAGGGGTAGAGAAGCCTAAATTTCTTCCTGATTTGTATATGACC14.0No Hit
CCTCCAATCTGTGTGCTGTGGCACATTTCCAAGAGAGATGCTAATGGGTC14.0No Hit
GGCAAGAGAAGAACCGCGCACTCAGAATGAAGTGGATGATGGCAATGAGA14.0No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)