FastQCFastQC Report
Fri 2 Oct 2020
HTGTLBGXG_n02_S2_R1_E05_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTGTLBGXG_n02_S2_R1_E05_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATTGGGTTTGATCCCACAGCTTCTTTAACTCAAATCATCTTCTCGTCTG14.761904761904762No Hit
GTCTAATGGAGCAAATGGAGTAAAAGGGTTTTCATTCAAATACGGCAATG14.761904761904762No Hit
TTGTTTATCCACATCAATATGCGCACCCGTAACGTCTTGTATAGCAGTGA14.761904761904762No Hit
GAAAGTTATTTAGTCACCCTAAATGTGAGTAGAAATTCAGAACTCTTTCA14.761904761904762No Hit
GCCTTATTGAATTCATTTTGGACCCAGCTTGCTAGAGATCTGGGCTCTGG14.761904761904762No Hit
GGGGGGATGGGCTTGCCGTCGCAAGTGGGCAGGACTTGGAATGGGAAGGG14.761904761904762No Hit
GTCCAAGATTCAGTATCGAGACTCCCAAGACCGTACTTAGCATGTTGAAC14.761904761904762No Hit
GTTCTAGCAGACGCTTTCTCCGTCTGTTGGAATGGGATGCTTTTCTCCAT14.761904761904762No Hit
CCCTCTTAACCCAACGTCCTGACTCTGTATAGAAAATGTGAAGAATCAGT14.761904761904762No Hit
CAAAATGACCCTATTAAAACTCCACAGCACTCGGATTTGACTGTGTCTAT14.761904761904762No Hit
GGATTACTGCTTGAATAAATGTTCGTTTGTAGAAACTATTAGACTCCGAG14.761904761904762No Hit
CTGTAAAACTCGACCGCGAAGGATTGCAGCTTGCGCTCGGGTCGAGGTCA14.761904761904762No Hit
GGGTACAGATAGCTTCAAATGAGAATGTGGAAAACATCGACTCCAATACC14.761904761904762No Hit
GTCCTACATTGTGGAAACACCTAGTTCAGACAATGGAACGTGTTACCCAG14.761904761904762No Hit
CCCTAAGGTATATAAAACTTATTTAGAAAAGCTCCAAAGCTTCGAACATG14.761904761904762No Hit
GTAGTCATCAGGCATGCGAGAGAAGCACACCTCAGTCACAGACAAGTGGA14.761904761904762No Hit
CCCTAAACCTCTCTGGGGATCTGACGTGTCTCCCTTCCCAATATCACCCA14.761904761904762No Hit
GGGTGACAAAAACATAATGGACTCCAACACCACGTCAAGCTTTCAGGTAG14.761904761904762No Hit
GGAAGGTTTGGATTGCAAATAGTATCATTACAGCAACAACTTAAGTGGAG14.761904761904762No Hit
ACTTCTGGTAGGCATGCAAATTTTCAAGAATGTCATATTTCAGACCAGCA14.761904761904762No Hit
GTAGAAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATCC14.761904761904762No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)