FastQCFastQC Report
Fri 2 Oct 2020
HTGTLBGXG_n02_S2_R1_E05-FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTGTLBGXG_n02_S2_R1_E05-FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences994706
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA2750.065.970961
TATTACG502.008421E-458.010612
GCGAAAG4750.038.1648752
GTATAGG2903.9108272E-1027.5258141
CCTATAT2756.697519E-926.3883841
CAATCCG1100.00966293726.368458145
CGAAAGC6900.026.2544213
TATATAC13750.025.8228933
ATATACT8200.025.6191444
TATACAG7450.025.2773785
GTATATA12500.023.802321
TATACTT7850.023.0666925
GTGCGTT1600.002172829122.660393145
CAGCTCG8350.021.6854539
TATACTG5700.021.6017555
CCCTTAC5002.3646862E-1120.3190541
GTATTAT6150.020.0594621
GTCCTAT2908.182775E-620.0187741
ATATATG8700.019.9895733
CTATAAG2556.907408E-519.9206431
Produced by FastQC (version 0.11.5)