FastQCFastQC Report
Fri 2 Oct 2020
HTGTLBGXG_n02_S2_R1_E01_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTGTLBGXG_n02_S2_R1_E01_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences57
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCTAGAGACCCGTTTTAGATATATAAGAAACCCAAAGAACCTATCACA11.7543859649122806No Hit
CACTTCGTTAGGGAAAACAACTTCCATGATTACATCCTGTGCCTCCTTGG11.7543859649122806No Hit
CTCCTCATGCTTGCGATATGTTCAATCGGGGCAACATCTTCTCCTATTTC11.7543859649122806No Hit
CTTCATATCCCTCATGCACTCTTATCTTCAATGTTTGAAGATTGCCCGTA11.7543859649122806No Hit
GGAGCACTGCATTCTACTTTGCTTTGGTGGCGCGGCTGCGAAGGGAAGAA11.7543859649122806No Hit
GTACACACTGCTTGTTCCACCAGCCACTGGGAGGAATCTCGTGTTGCGGC11.7543859649122806No Hit
ACAGTGGGTTTGTAAGAACTCTGTTCCAACAAATGAGGGATGTGCTTGGG11.7543859649122806No Hit
CTACATGACGAGAACGCAGAAATGATGAAGTTTACAAAATTAATAATATC11.7543859649122806No Hit
GGCTGCAATGGGCCTGAGAATTAGCTCATCCTTCACTTTTGGTGGATTCA11.7543859649122806No Hit
GAAGTCCGCGCTCTAATGAATAAGTCTTGAACGCGCGGCTTAATCCCTAA11.7543859649122806No Hit
CAAAGACAGTTCATTATCCACAAATCTACAAAACTGATTTTGAACGAGTC11.7543859649122806No Hit
ATCCCAATCATTCCAATACCCTGGTCGATAGGTTCCAATACCCAATNNNN11.7543859649122806No Hit
GTTCAACTCCCCAATTTTGAAAAAGCACTTTCGCATCCGTATGAAAATGT11.7543859649122806No Hit
ATACATCTGTTCCCAGCATGTTCCTTCACTCAAAAGCAACACAACAAAGT11.7543859649122806No Hit
ACCCGATTGAAGGACCACTACCTGAGAATAATTCACCAAGTGTGTATGCA11.7543859649122806No Hit
TTCCAGAACATCGTGATTAAATACTACTCTAACAGGATTTGATAACTCTA11.7543859649122806No Hit
GAATTGACAAATAACAAATACAGTTCATTATCCAAAAATCTACAAAACTT11.7543859649122806No Hit
ACACCACGTCTCCTAGCCCAATTAGCCCAATAGCCTTCTCTCCTTTCGCA11.7543859649122806No Hit
CCTCAGGCGTGCGATATGTTCAAGCGGGGCCACATCTTCTCCTATTTCAT11.7543859649122806No Hit
GTCTCTTGGTGGCATTGTTAGAGTTGAATCCAGGACAATTGCCCCTTACC11.7543859649122806No Hit
GTTCACAATGGTTGGGTGAAGAGGAACAGCCATAGTCAGAAAAGCAAGCA11.7543859649122806No Hit
ATCCTATGCATAAACTTTTAAGACATTTTCAGAAGGATGCGAAAGTGCTT11.7543859649122806No Hit
AATGTATGCAACCATCAAAGGAGAAATTTTTCAATCCTGGAGTTCTTCTT11.7543859649122806No Hit
GAACCGAGCAACGGCCACTAACTGACTCAGCGTCAATTAGTTGTGATATT11.7543859649122806No Hit
ATTCAATATGGAAAGAATAAAAGAACTAAGAAATCTAATGTCGCAGTCTC11.7543859649122806No Hit
GTCCAAGAGGGATAAGAACATTTAACAATTTGTTCTTGAAGATAAACAGC11.7543859649122806No Hit
CACAAGAATTAACCTATGCCTTATTTTGATAAAATAACGATTCAACAAGG11.7543859649122806No Hit
GTTGTTCATTTCCGCGTTGGGGCGTGGTTCTCGAAACGGGGACACTCCGC11.7543859649122806No Hit
ATTATAAACAAAGTAGTTTGAAAATCTAGAAATATAGTGACAAAGTGACG11.7543859649122806No Hit
GAGTATGGATCTTGCACTTCTCCCAACCATTGTGAAAACTTCATATCCCT11.7543859649122806No Hit
ATCCTTGAGTTCTTCTTTCTTCTATTTGGTTATCGTTATTTGCGATTCCG11.7543859649122806No Hit
AACCATTTCAGTCTTTAGTACCTAAGCCCATTAGAGGCCAATACAGTGGG11.7543859649122806No Hit
CCGTGGACCATATGGCCATAATCAAGAAGGTCCCATCAGGAAGCCAGGGG11.7543859649122806No Hit
ATTCCTCCCAGTGGATGGTGGAACAAGCAGTGTGTACATTGAAGTGTTGC11.7543859649122806No Hit
GTCGATAGTTTCAACGACCCAATTTTGAAAAAGCACTTTCGCATACTTAT11.7543859649122806No Hit
CACATTCACAGTAAATGAGGAGAACTGCATTCTACTTTGCTTTGGTGGAG11.7543859649122806No Hit
GATGTGGATATTGACCCTACGCTTTTACTCAATTAATAATACCCAATATT11.7543859649122806No Hit
GCTAATTCTCAGTCCCATTGCAGCCTTGCATATATCCACGGCTTGCTCTT11.7543859649122806No Hit
TATTTAAACAATAAGTCACAAATACCACACCTTCCTCGAAATAGTATTCT11.7543859649122806No Hit
TCTGAGGGGATTCCTCATTCTGGGCAAAGAAGACAAGAGATATGGGCCAG11.7543859649122806No Hit
CTACAGCACGGAGAGGGTAGTGGTGAGCATTGACCTTTTTTTGAGAATCC11.7543859649122806No Hit
ACTGTTAACCATATGGCCATAATCAAAAACTACACAACACCAAGGCAACA11.7543859649122806No Hit
GGCATATGAAGAGTTCACAGTGCTTGCGAGAAGAGCAAAAGCCAAAATCA11.7543859649122806No Hit
GTCAAAGGATGTAATCAAGGAAGTTCTTTTCACTAACGAAGTGGGATCCA11.7543859649122806No Hit
GTATATTAAAAAGAGGATTGTATGAAAAAAGCAGTCAGAGGTGATATGAA11.7543859649122806No Hit
ATCCCAATGCGCAGACACTGTATTACACACGACTTCATCATTAGACAAAA11.7543859649122806No Hit
GTCACAAGCACATCCCTCATTTGTTTGAACAGAGTTCTTACAAAAACACT11.7543859649122806No Hit
GATTCGGTTCATGGAATTTGGAGTAACAGGGAGGTGTCTCGACATATATT11.7543859649122806No Hit
GTGAATGTGAGGGGATCAGGAATGAGAATACTTGTAAGGGGCAATTCTCC11.7543859649122806No Hit
CATGTAGGAGAACACGCGGCTCATCAGTCAAGAGAGCGGAATATGTGCGT11.7543859649122806No Hit
CGACTGTTCGTATCTCCCACTCACTATCCGCTGCATCAATCTCCTGGTTG11.7543859649122806No Hit
AACCGGGGTCGTTTCCAAGAAGGTTCAGTCGTTTGGTTGACTGGGTGGAA11.7543859649122806No Hit
ATTCCGAAGTTAGTATCCAGGCTCCCACTTCGTTAGGGAAAACAACTTCC11.7543859649122806No Hit
CATTACAAGTATTCTCATTCCTGATGCCCTCACATTCACAGTAAATGTGG11.7543859649122806No Hit
AATTCAACCATTTAACAGCAAGAGGTGATCCCATCACTCTGTCTGATACT11.7543859649122806No Hit
GAATTGCCCCTTACAACTATTCTCATTCCTGATCCCCTCACATTCACAGT11.7543859649122806No Hit
GAGTAAGTTATTGTCAGTTTCTCTGTTCCCTGTCTTTCACTGACCTCCTC11.7543859649122806No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAAGAAT50.0145.03
CACAAGA50.0145.01
AATTAAC50.0145.07
AGAATTA50.0145.05
ATTAACC50.0145.08
AAGAATT50.0145.04
GAATTAA50.0145.06
ACAAGAA50.0145.02
TTAACCT50.0145.09
AAAATAA150.048.333332145
Produced by FastQC (version 0.11.5)